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Quencher-free molecular beacons as probes for oligonucleotides containing CAG repeat sequences [PDF]
Chemical Communications, 2014 Quencher-free molecular beacons allow the sensitive probing of DNA and RNA CAG repeat oligonucleotides with significant increases in fluorescence intensity of 16- and 43.5-fold, respectively.
Rakesh N Veedu +2 more
exaly +6 more sources
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Facile conversion of ATP-binding RNA aptamer to quencher-free molecular aptamer beacon
Bioorganic and Medicinal Chemistry Letters, 2018We have developed RNA-based quencher-free molecular aptamer beacons (RNA-based QF-MABs) for the detection of ATP, taking advantage of the conformational changes associated with ATP binding to the ATP-binding RNA aptamer. The RNA aptamer, with its well-defined structure, was readily converted to the fluorescence sensors by incorporating a fluorophore ...
Tirayut Vilaivan +2 more
exaly +4 more sources
Intermolecular G-quadruplex-based universal quencher free molecular beacon
Chemical Communications, 2012A simple and universal quencher-free molecular beacon (MB) with low background fluorescence is developed based on an intermolecular G-quadruplex signaling probe. Unlike previous fluorescent MB strategies, it can function without any fluorophore and quencher modifications on its hairpin sequence.
Hui Zhou, Ji-Shan Li, Zai-Sheng Wu
exaly +3 more sources
A Highly Discriminating Quencher-Free Molecular Beacon for Probing DNA
Journal of the American Chemical Society, 2004We inserted a fluorene-labeled deoxyuridine derivative, synthesized using Sonogashira coupling, efficiently into the loop region of a DNA hairpin using phosphoramidite chemistry. This molecular beacon, which features no additional fluorescence quencher, discriminates between perfect and one-base-mismatched base pairing by changes in its fluorescence ...
Hwang, GT, Seo, YJ, Kim, BH
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A quencher-free DNAzyme beacon for fluorescently sensing uranyl ions via embedding 2-aminopurine
Biosensors and Bioelectronics, 2019DNAzyme-based fluorescent probes have provided valuable protocols for detecting uranium, one of the most common radioactive contaminants in the environment, with ultra-high selectivity and sensitivity. Designing novel DNAzyme beacons to update the mode of fluorescence reporting and/or quenching will continuously enhance "turn-on" sensing performance as
Wei Tang, , Jun Fu
exaly +3 more sources
Triad Base Pairs Containing Fluorene Unit for Quencher‐Free SNP Typing.
ChemInform, 2007AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Jin Ho Ryu +4 more
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Quencher-Free, End-Stacking Oligonucleotides for Probing Single-Base Mismatches in DNA
Organic Letters, 2005[reaction: see text] Fluorescence can be quenched through PET from the fluorophore to neighboring C, T, and G bases, but not to the A moiety. The hairpin stem stability arising from pi-stacking and the PET between the pyrene-labeled 2'-deoxynucleotide units and their neighboring bases are the two main factors that affect the operation of these novel ...
Seo, YJ, Ryu, JH, Kim, BH
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Quencher-free molecular beacon systems with two pyrene units in the stem region
Tetrahedron Letters, 2006Abstract We appended pyrene units covalently onto adenosine and uridine nucleosides (forming A P and U P units, respectively) and then incorporated them into oligonucleotides such that they were positioned in complementary locations in opposite strands in the middle positions of hairpin stems. Systems 1 ( A P U P ) and 3 ( A P
Young Jun Seo +2 more
exaly +3 more sources
A Quencher‐Free Linear Probe from Serinol Nucleic Acid with a Fluorescent Uracil Analogue
ChemBioChem, 2019AbstractWith the goal of developing a quencher‐free probe composed of an artificial nucleic acid, the fluorescent nucleobase analogue 5‐(perylenylethynyl)uracil (PeU), which was incorporated into totally artificial serinol nucleic acid (SNA) as a substitute for thymine, has been synthesized.
Keiji Murayama, Hiroyuki Asanuma
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Analytical Chemistry, 2016
We describe a facile quencher-free fluorescence strategy for rapid detection of microRNAs (miRNAs) by using a novel double-strand displacement sensor. The sensor is designed with an outstanding 2-aminopurine (2-AP) fluorophore as a probe and a predesigned cDNA, which can completely complement the target miRNA and partly complement the 2-AP probe.
Rong, Liao +4 more
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We describe a facile quencher-free fluorescence strategy for rapid detection of microRNAs (miRNAs) by using a novel double-strand displacement sensor. The sensor is designed with an outstanding 2-aminopurine (2-AP) fluorophore as a probe and a predesigned cDNA, which can completely complement the target miRNA and partly complement the 2-AP probe.
Rong, Liao +4 more
openaire +2 more sources