Results 221 to 230 of about 22,884 (241)
Some of the next articles are maybe not open access.
Analytical Chemistry, 2014
Sensitive and accurate analysis of microRNA (miRNA) expression is imperative for understanding the biological functions of miRNAs and the early diagnosis of human cancers. Here, we develop a quencher-free fluorescent method for homogeneously sensitive detection of let-7a miRNA using the target-triggered recycling signal amplification in combination ...
Guichi, Zhu, Li, Liang, Chun-yang, Zhang
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Sensitive and accurate analysis of microRNA (miRNA) expression is imperative for understanding the biological functions of miRNAs and the early diagnosis of human cancers. Here, we develop a quencher-free fluorescent method for homogeneously sensitive detection of let-7a miRNA using the target-triggered recycling signal amplification in combination ...
Guichi, Zhu, Li, Liang, Chun-yang, Zhang
openaire +2 more sources
Analytical Chemistry, 2020
Circulating miRNAs are a newly emerging class of noninvasive biomarkers, and the accurate quantification of their expression is essential to the biological research and early clinic diagnosis. Herein, we demonstrate the construction of a quencher-free cascade amplification system for highly sensitive detection of serum circulating miRNAs.
Li-juan Wang +5 more
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Circulating miRNAs are a newly emerging class of noninvasive biomarkers, and the accurate quantification of their expression is essential to the biological research and early clinic diagnosis. Herein, we demonstrate the construction of a quencher-free cascade amplification system for highly sensitive detection of serum circulating miRNAs.
Li-juan Wang +5 more
openaire +2 more sources
Japanese Journal of Applied Physics, 2003
In the experiment of antiphotofading of DVD-R optical recording disc, a 3000 W xenon light (λ=420 nm) was adopted for testing the parameters of a novel cyanine-TCNQ (tetracyanoquinodimethane) charge transfer complex as a recording layer. Its performance without addition of a singlet oxygen quencher under illumination of 1200 min still satisfies with ...
Wen-Yih Liao +3 more
openaire +1 more source
In the experiment of antiphotofading of DVD-R optical recording disc, a 3000 W xenon light (λ=420 nm) was adopted for testing the parameters of a novel cyanine-TCNQ (tetracyanoquinodimethane) charge transfer complex as a recording layer. Its performance without addition of a singlet oxygen quencher under illumination of 1200 min still satisfies with ...
Wen-Yih Liao +3 more
openaire +1 more source
Bioorganic and Medicinal Chemistry, 2013
A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.
Jørgen Kjems, Jesper Wengel
exaly +5 more sources
A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of RNA in living cells was demonstrated.
Jørgen Kjems, Jesper Wengel
exaly +5 more sources
Bioorganic & Medicinal Chemistry, 2012
In this study, we synthesized a simple but efficient quencher-free molecular beacon tethering 7-hydroxycoumarin on D-threoninol based on its pK(a) change. The pK(a) of 7-hydroxycoumarin in a single strand was determined as 8.8, whereas that intercalated in the duplex was over 10.
Hiromu, Kashida +3 more
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In this study, we synthesized a simple but efficient quencher-free molecular beacon tethering 7-hydroxycoumarin on D-threoninol based on its pK(a) change. The pK(a) of 7-hydroxycoumarin in a single strand was determined as 8.8, whereas that intercalated in the duplex was over 10.
Hiromu, Kashida +3 more
openaire +2 more sources
The Analyst, 2016
We report an analytical method that exploits the interaction between G-quadruplexes and thioflavin T (ThT), for detecting mutant DNA species containing single-base changes.
Il Joon, Lee, Nam-In, Goo, Dong-Eun, Kim
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We report an analytical method that exploits the interaction between G-quadruplexes and thioflavin T (ThT), for detecting mutant DNA species containing single-base changes.
Il Joon, Lee, Nam-In, Goo, Dong-Eun, Kim
openaire +2 more sources
Journal of Pharmaceutical and Biomedical Analysis, 2018
We fabricated a quencher-free and enzyme-free fluorescence detection system by employing the DNA logic circuits as signal amplifier and 2-aminopurine as signal indicator, and applied it to detect DNA and adenosine triphosphate (ATP). The assay system consisted of three hairpin probes with a sequestered 2-aminopurine molecule in each stem domain which ...
Shijia Ding
exaly +3 more sources
We fabricated a quencher-free and enzyme-free fluorescence detection system by employing the DNA logic circuits as signal amplifier and 2-aminopurine as signal indicator, and applied it to detect DNA and adenosine triphosphate (ATP). The assay system consisted of three hairpin probes with a sequestered 2-aminopurine molecule in each stem domain which ...
Shijia Ding
exaly +3 more sources
Quencher-free linear beacon systems containing 2-ethynylfluorenone-labeled 2′-deoxyuridine units
Tetrahedron, 2012Abstract In this study we synthesized the 2-ethynylfluorenone-containing deoxyuridine derivative U FO and incorporated it at the central positions of oligodeoxynucleotides (ODNs) in an attempt to develop a new type of quencher-free linear beacon probe and to understand the effect of functionalization of the fluorene scaffold on the photophysical ...
Ryu, JH +4 more
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Organic & Biomolecular Chemistry, 2017
pH-Responsive oligodeoxynucleotides only exhibited dramatic increases in fluorescence upon duplex formation with their fully matched target DNAs.
Ji Won Lee +4 more
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pH-Responsive oligodeoxynucleotides only exhibited dramatic increases in fluorescence upon duplex formation with their fully matched target DNAs.
Ji Won Lee +4 more
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Analytical and Bioanalytical Chemistry
MicroRNAs (miRNAs) have emerged as crucial post-transcriptional regulators of gene expression and promising biomarkers for disease diagnosis and prognosis. Their aberrant expression levels are closely associated with the pathogenesis and progression of various human diseases.
Dan Wang +4 more
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MicroRNAs (miRNAs) have emerged as crucial post-transcriptional regulators of gene expression and promising biomarkers for disease diagnosis and prognosis. Their aberrant expression levels are closely associated with the pathogenesis and progression of various human diseases.
Dan Wang +4 more
openaire +2 more sources