Results 181 to 190 of about 16,571 (204)

Trypanosoma cruzi: Cloning and Characterization of a RAB7 gene

Experimental Parasitology, 2000
The small monomeric GTP-binding proteins of the RAB subfamily are key regulatory elements of the machinery that controls membrane traffic in eukaryotic cells. These proteins have been localized to many different intracellular organelles, on both endocytic and exocytic compartments, suggesting that each step of vesicular traffic can involve a specific ...
S T, Leal, J R, Araripe, T P, Urményi, G A, Cross, E, Rondinelli   +4 more
openaire   +2 more sources

Rab7 regulates phagosome maturation in Dictyostelium

Journal of Cell Science, 2001
A Dictyostelium Rab7 homolog has been demonstrated to regulate fluid-phase influx, efflux, retention of lysosomal hydrolases and phagocytosis. Since Rab7 function appeared to be required for efficient phagocytosis, we sought to further characterize the role of Rab7 in phagosomal maturation.
A, Rupper, B, Grove, J, Cardelli
openaire   +2 more sources

Dissection of Rab7 localization on Mycobacterium tuberculosis phagosome

Biochemical and Biophysical Research Communications, 2009
The late endosomal marker Rab7 has been long believed to be absent from the phagosome containing Mycobacterium tuberculosis (M.tb) in macrophage, but the detail kinetics remains elusive. Here, we found that Rab7 is transiently recruited to and subsequently released from M.tb phagosomes.
Shintaro, Seto, Sohkichi, Matsumoto, Isamu, Ohta, Kunio, Tsujimura, Yukio, Koide   +4 more
openaire   +2 more sources

Structural mechanism for regulation of Rab7 by site-specific monoubiquitination

International Journal of Biological Macromolecules, 2022
Site-specific ubiquitination can regulate the functions of Rab proteins in membrane trafficking. Previously we showed that site-specific monoubiquitination on Rab5 downregulates its function. Rab7 acts in the downstream of Rab5. Although site-specific ubiquitination of Rab7 can affect its function, it remains elusive how the ubiquitination is involved ...
Jung, J., Baek, J., Tae, K., Shin, D., Han, S., Yang,Wonjin, Yu, Wookyung, Jung, S.M., Park, S.H., Choi, C.Y., Lee, S.   +10 more
openaire   +3 more sources

Human VPS34 and p150 are Rab7 Interacting Partners

Traffic, 2003
Regulation of membrane trafficking requires the concerted actions of rab proteins, their effectors and several phosphatidylinositol 3′‐kinases. Rab7 is required for late endosomal transport and here we establish that the phosphatidylinositol 3′‐kinase hVPS34 and its adaptor protein p150 are rab7 interacting partners. The hVPS34/p150 complex colocalized
Mary-Pat, Stein, Yan, Feng, Karen L, Cooper, Angela M, Welford, Angela, Wandinger-Ness   +4 more
openaire   +2 more sources

Cloning and expression analysis of the murine Rab7 cDNA

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1995
A cDNA clone coding for the Rab7 protein was isolated from an NIH3T3 cell line (mouse fibroblasts) cDNA library. Sequence analysis shows high homology to the rat and dog cDNAs. Northern blot analysis showed the presence of two messenger RNA that differ at the 3' untranslated region.
VITELLI R, CHIARIELLO M, BRUNI CB, BUCCI, Cecilia   +3 more
openaire   +5 more sources

Intein-Mediated Synthesis of Geranylgeranylated Rab7 Protein in Vitro

Journal of the American Chemical Society, 2002
Production of recombinant proteins is an important prerequisite for biotechnology and life sciences in general. However, there is a paucity of methods for production of posttranslationally modified recombinant proteins or proteins with non-native functional groups, such as fluorophores, spin labels, and so forth. In this work we have used a combination
Alexandrov, K., Heinemann, I., Durek, T., Sidorovitch, V., Goody, R. S., Waldmann, H.   +5 more
openaire   +6 more sources

Synthesis of Fluorescently Labeled Mono- and Diprenylated Rab7 GTPase

Journal of the American Chemical Society, 2004
Modification of proteins with isoprenoid lipids is a widespread phenomenon in eukaryotic organisms that has received much attention due to its involvement in the progression of several diseases including cancer. Progress in studies of prenylated proteins has been hampered by difficulties associated with isolation of these proteins from native or ...
Durek, T., Alexandrov, K., Goody, R. S., Hildebrand, A., Heinemann, I., Waldmann, H.   +5 more
openaire   +6 more sources

The rab7 gtpase resides on a vesicular compartment connected to lysosomes

Journal of Cell Science, 1995
ABSTRACT Rab GTPases belong to the Ras GTPase superfamily and are key regulators of membrane traffic. Among them, rab7 has been localized on late endosomes of NRK cells but its function remains unknown. In order to investigate its role, we generated stable HeLa cell lines that express either wild type or a GTPase-defective mutant of rab7
S, Méresse, J P, Gorvel, P, Chavrier
openaire   +2 more sources

Inductive Process Modeling of Rab5-Rab7 Conversion in Endocytosis

2013
Inductive process modeling (IPM) is an approach to equation discovery that can be used to induce comprehensible models of dynamical systems from observed data and domain knowledge. We apply IPM to the task of modeling the conversion of Rab5 domain proteins to Rab7 domain proteins, a key process in endocytosis.
Jovan Tanevski, Ljupco Todorovski, Yannis Kalaidzidis, Saso Dzeroski   +3 more
openaire   +1 more source