Results 11 to 20 of about 822,055 (188)

Fully Automated Radioligand Binding Filtration Assay for Membrane-Bound Receptors

BioTechniques, 2002
Here we describe a fully automated, hands-free radioligand filtration binding assay for dopamine D3 receptors. Three separate instruments were linked in tandem to perform the following operations: The Genmate and Genesis were linked to perform liquid ...
A. Shrikhande, C. Courtney, D. Smith, M. Melch, M. McConkey, J. Bergeron, Stephen K-F. Wong   +6 more
doaj   +2 more sources

Synthesis of Sulochrin-125I and Its Binding Affinity as α-Glucosidase Inhibitor using Radioligand Binding Assay (RBA) Method [PDF]

Atom Indonesia, 2014
Most of diabetics patients have type 2 diabetes mellitus or non insulin dependent diabetes mellitus. Treatment type 2 diabetes mellitus can be done by inhibiting α-glucosidase enzyme which converts carbohydrates into glucose.
W. Lestari, V.Y. Susilo, S. Setiyowati, Triningsih, A. Ariyanto, P. Widayati, L.B.S. Kardono, A. Yanuar3   +7 more
doaj   +6 more sources

Paltusotine versus octreotide: different effects on radioligand uptake in neuroendocrine tumours [PDF]

Endocrine Oncology
Objective: Somatostatin receptor analogues are well-established in the treatment of metastatic gastro-enteropancreatic neuroendocrine tumours (GEP-NETs), especially for symptom control in patients with the carcinoid syndrome, and to control tumour growth.
Katharina Wang, Christoph J Auernhammer, Simon Lindner, Mathias Zacherl, Rudolf A Werner, Julian Maurer, Lea Peischer, Julia Hamati, Maximilian P Hungbauer, Thomas Knösel, Astrid Reul, Elena Kuzmenko, Diana Vetter, José Oberholzer, Umberto Maccio, Felix Beuschlein, Martin Reincke, Constanze Hantel, Karel Pacak, Ashley B Grossman, Kathrin Zitzmann, Svenja Nölting   +21 more
doaj   +2 more sources

Towards the Development of a Targeted Albumin-Binding Radioligand for Theranostic Applications: Synthesis, Radiolabelling and Preliminary In Vivo Studies [PDF]

, 2020
GluCAB is a newly synthesised macrocyclic radioligand designed to bind in vivo to albumin (HSA) for the purpose of development as a dual-targeting oncological theranostic agent.
Iqbal, Parker, Zoltan, Szucs, Thomas, Ebenhan, Jan Rijn, Zeevaart, Roger, Hunter, Cathryn H.S., Driver   +5 more
core   +2 more sources

A partially automated radioligand binding assay system for use in clinical and pharmaceutical research [PDF]

, 1993
Using a Tecan robotic sample processor and IBM compatible PCs we have developed a flexible, partially automated radioligand binding assay system. It handles pipetting parameters of up to 16 saturation or competition experiments at a time with up to 24 ...
Liebl, B., Anhäupl, T., Haen, Ekkehard, Georgieff, M., Günster, B.   +4 more
core   +1 more source

[3H]-Spiperone Competition Binding to Dopamine D2, D3 and D4 Receptors

Bio-Protocol, 2013
This protocol is intended for use in 96 well plates (1,200 μl wells) but it can similarly be applied to standard test tubes (Levant, 2007). D2, D3, and D4 dopamine receptors are members of the D2-like class of dopamine receptors.
Jan-Peter Wieringen, Martin Michel
doaj   +1 more source

FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter Resolution

Bio-Protocol, 2021
Secondary active transporters reside in cell membranes transporting polar solutes like amino acids against steep concentration gradients, using electrochemical gradients of ions as energy sources.
Didar Ciftci, Gerard Huysmans, Xiaoyu Wang, Changhao He, Daniel Terry, Zhou Zhou, Gabriel Fitzgerald, Scott Blanchard, Olga Boudker   +8 more
doaj   +1 more source

Application of the Gyrolab microfluidic platform to measure picomolar affinity of a PD-L1-binding Adnectin™ radioligand for positron emission tomography

BioTechniques, 2020
Advances in in vitro display and protein engineering yield therapeutics with affinities in the picomolar range. The Gyrolab® microfluidics platform uses the kinetic exclusion assay principle to measure subnanomolar solution affinities.
Zhou Dai, Juhi Juneja, Lumelle Schneeweis, Daniel Cohen, Frank Marsilio, Paul Morin, Ruchira DasGupta   +6 more
doaj   +1 more source

Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis [PDF]

, 2001
An assay device for the rapid detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked ...
Smits, H. L., Kuriakose, M., Abdoel, Theresia H, Gasem, M. Hussein, Sugathan, Sheela, Smits, Henk L., Pujianto, B., Sasaki, David, Sasaki, D., Vestering, Marc, Abdoel, T. H., Kuriakose, Mariamma, Gussenhoven, G. C., Eapen, C. K., Gasem, M. H., Pujianto, Bambang, Yersin, C., Gussenhoven, George C, Sugathan, S., Vestering, M., Yersin, Claude   +20 more
core   +2 more sources

Marine Natural Products Acting on the Acetylcholine-Binding Protein and Nicotinic Receptors: From Computer Modeling to Binding Studies and Electrophysiology

Marine Drugs, 2014
For a small library of natural products from marine sponges and ascidians, in silico docking to the Lymnaea stagnalis acetylcholine-binding protein (AChBP), a model for the ligand-binding domains of nicotinic acetylcholine receptors (nAChRs), was ...
Denis Kudryavtsev, Tatyana Makarieva, Natalia Utkina, Elena Santalova, Elena Kryukova, Christoph Methfessel, Victor Tsetlin, Valentin Stonik, Igor Kasheverov   +8 more
doaj   +1 more source