RAS-inhibiting biologics identify and probe druggable pockets including an SII-α3 allosteric site
Nature Communications, 2021 Oncogenic RAS mutants remain difficult to target with small molecules. Here, the authors show that RAS-binding Affimer proteins inhibit RAS signaling while binding diverse regions on the RAS surface, suggesting the potential to use Affimers as tools to ...
Katarzyna Z. Haza +20 more
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Applying antibodies inside cells: Principles and recent advances in neurobiology, virology and oncology [PDF]
, 2020 To interfere with cell function, many scientists rely on methods that target DNA or RNA due to the ease with which they can be applied. Proteins are usually the final executors of function but are targeted only indirectly by these methods.
Marschall, Andrea +4 more
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Deconstruction of the Ras switching cycle through saturation mutagenesis
eLife, 2017 Ras proteins are highly conserved signaling molecules that exhibit regulated, nucleotide-dependent switching between active and inactive states. The high conservation of Ras requires mechanistic explanation, especially given the general mutational ...
Pradeep Bandaru +10 more
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Molecules linked to Ras signaling as therapeutic targets in cardiac pathologies
Biological Research, 2021 Highlights The Ras (Rat Sarcoma) gene family is a group of small G proteins Ras is regulated by growth factors and neurohormones affecting cardiomyocyte growth and hypertrophy Ras directly affects cardiomyocyte physiological and pathological hypertrophy ...
Manuel Ramos-Kuri +5 more
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Signal integration by lipid-mediated spatial cross talk between Ras nanoclusters [PDF]
, 2013 Lipid-anchored Ras GTPases form transient, spatially segregated nanoclusters on the plasma membrane that are essential for high-fidelity signal transmission. The lipid composition of Ras nanoclusters however has not previously been investigated.
Abankwa +65 more
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Gi- and Gs-coupled GPCRs show different modes of G-protein binding. [PDF]
, 2018 More than two decades ago, the activation mechanism for the membrane-bound photoreceptor and prototypical G protein-coupled receptor (GPCR) rhodopsin was uncovered.
Altenbach, Christian +7 more
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Ras-GRF Activates Ha-Ras, but Not N-Ras or K-Ras 4B, Proteinin Vivo [PDF]
Journal of Biological Chemistry, 1998 Human cells contain four homologous Ras proteins, but it is unknown whether these homologues have different biological functions. As a first step in determining if Ras homologues might participate in distinct signaling cascades, we assessed whether a given Ras guanine nucleotide exchange factor could selectively activate a single Ras homologue in vivo.
M K, Jones, J H, Jackson
openaire +2 more sources
A Comparative Analysis of Individual RAS Mutations in Cancer Biology
Frontiers in Oncology, 2019 In human cells, three closely related RAS genes, termed HRAS, KRAS, and NRAS, encode four highly homologous proteins. RAS proteins are small GTPases involved in a broad spectrum of key molecular and cellular activities, including proliferation and ...
Carmen Muñoz-Maldonado +5 more
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TGFβ-induced changes in membrane curvature influence Ras oncoprotein membrane localization
Scientific Reports, 2022 In the course of cancer progression tumor cells undergo morphological changes that lead to increased motility and invasiveness thus promoting formation of metastases.
Alexandros Damalas +3 more
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Purification and characterization of human H-RAS proteins expressed in Escherichia coli [PDF]
, 1985 The full-length normal and T24 mutant human H-ras proteins and two truncated derivatives of the T24 mutant were expressed efficiently in Escherichia coli. The proteins accumulated to 1 to 5% of total cellular protein, and each was specifically recognized
Gross, M. +7 more
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