Results 81 to 90 of about 226,892 (266)

MIRATE: MIps RATional dEsign Science Gateway

Journal of Integrative Bioinformatics, 2018
Molecularly imprinted polymers (MIPs) are high affinity robust synthetic receptors, which can be optimally synthesized and manufactured more economically than their biological equivalents (i.e. antibody).
Busato Mirko, Distefano Rosario, Bates Ferdia, Karim Kal, Bossi Alessandra Maria, López Vilariño José Manuel, Piletsky Sergey, Bombieri Nicola, Giorgetti Alejandro   +8 more
doaj   +1 more source

Rational design of allosteric ribozymes

Chemistry & Biology, 1997
Efficient operation of cellular processes relies on the strict control that each cell exerts over its metabolic pathways. Some protein enzymes are subject to allosteric regulation, in which binding sites located apart from the enzyme's active site can specifically recognize effector molecules and alter the catalytic rate of the enzyme via ...
Tang, Jin, Breaker, Ronald R.
openaire   +2 more sources

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Engineering a Thermostable Reverse Transcriptase for RT-PCR Through Rational Design of Pyrococcus furiosus DNA Polymerase

Biomolecules
Engineering of a bifunctional enzyme that combines DNA-dependent DNA polymerase and reverse transcriptase (RT) activities is a highly promising biotechnological goal, as it would enable one-enzyme RT-PCR.
Aleksandra A. Kuznetsova, Irina A. Grishina, Elena S. Mikushina, Nikita A. Kuznetsov   +3 more
doaj   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source

Carbon-based Nanozymes: How Structure Affects Performance

Nano Biomedicine and Engineering
In the past decade, nanozymes - a unique class of nanomaterials with inherent enzyme-mimetic properties - have fascinated researchers, revealing unexpected enzyme-like activity of nanomaterials previously considered biologically inert.
Jiuyang He, Yinyin Hou, Zixia Zhang, Junying Zhang, Xiyun Yan, Kelong Fan, Minmin Liang   +6 more
doaj   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source

Rational Design to Improve D-Psicose-3-epimerase Thermal Stability of Blautia wexlerae

Shipin gongye ke-ji
To develop a novel D-psicose-3-epimerase possessing higher thermal stability, an exploration was carried out on the genome of the intestinal probiotic Blautia wexlerae.
Kaixuan RAN, Mingqiang XUN, Kaixin LIN, Yu FU, Lili WANG, Chunxiao ZHANG, Hong YI   +6 more
doaj   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

RATIONAL DESIGN OF NONLINEAR-DEFORMABLE STRUCTURALLYHETEROGENEOUS ELEMENTS OF STRUCTURES

International Journal for Computational Civil and Structural Engineering
The article deals with the problem of rational design of a nonlinearly deformable heterogeneous composite Timoshenko rod under force impact. The rod has a structure that is symmetrical relative to the force plane, formed by the connection of quasi ...
Andrey Mishchenko, Maxim Veshkin, Vladimir Mondrus, Leonid Stupishin   +3 more
doaj   +1 more source