Results 81 to 90 of about 82,493 (288)

Binding of PI3KC2β-RBD to Ras.

, 2013
(A) Nucleotide loaded Ras does not directly interact with the RBD of PI3KC2β. Ras-GDP or Ras-GTPγS were incubated with GST-Raf-RBD, GST-PI3KC2β-RBD or GST alone as a negative control. Bound proteins were then analyzed by Western blot with a Ras antibody.
Katy A. Wong (168298), John P. O'Bryan (168331), Yun-Ju Chen (216237), Arnon Lavie (307968), Angela Russo (168307), Xuerong Wang (168318)   +5 more
core   +1 more source

A Generative Neuro‐Symbolic AI for Protein Sequence Design

Advanced Science, EarlyView.
We introduce EffieDes, a neuro‐symbolic framework coupling deep learning‐based fitness landscape parameterization with exact automated reasoning. Unlike greedy sampling, EffieDes identifies sequences that globally optimize fitness while satisfying intricate design constraints.
Marianne Defresne, Delphine Dessaux, Samuel Buchet, Lucie Barthe, Liza Ammar‐Khodja, Bessam Azizi, Valentin Durante, Gianluca Cioci, Simon de Givry, Alain Roussel, Luis F. Garcia‐Alles, Thomas Schiex, Sophie Barbe   +12 more
wiley   +1 more source

dsRADAR: Imaging and Detecting Cellular dsRNA by Repurposing RNA‐Binding Proteins

Angewandte Chemie, EarlyView.
Double‐stranded RNA (dsRNA) plays an important role in the innate immune system, but can be misregulated in disease, leading to inflammation. Despite the importance of this motif, methods for detecting dsRNA remain limited. This work shows that ADAR3 can be repurposed to provide improved detection of dsRNA, which is demonstrated in the context of viral
Weina Cheng, Tyson D. Todd, Alexandria L. Quillin, Harshad Ingle, Angela M. Halstead, Megan T. Baldridge, José B. Sáenz, Jennifer M. Heemstra   +7 more
wiley   +2 more sources

Impact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian Cells

, 2022
SARS-CoV-2 receptor-binding domain (RBD) is a major target for the development of diagnostics, vaccines and therapeutics directed against COVID-19. Important efforts have been dedicated to the rapid and efficient production of recombinant RBD proteins ...
Jerome Martinez, Dimino, Johanna, Maxime Combe, Soizic Daniel, Combe, Maxime, Sandra Bouanich, Johanna Dimino, Steinbrugger, Christophe, Christophe Steinbrugger, Daniel, Soizic, Corine Piegay, Martinez, Jerome, Gerez, Guillaume, Berthier, Franck, Guillaume Gerez, Piegay, Corine, Bouanich, Sandra, Franck Berthier   +17 more
core   +1 more source

Somatic Evolution of a Germline Antibody Expands its Breadth to Neutralize Early SARS‐CoV‐2 Omicron Variants

Advanced Science, EarlyView.
Engineering of the 148‐germline (148‐GL) antibody reveals a roadmap for overcoming SARS‐CoV‐2 viral escape. By introducing four specific somatic hypermutations, the resulting 148‐M4 variant restores potent neutralization against Omicron BA.1 and BA.4/5.
Huibin Lv, Ziqi Feng, Qi Wen Teo, Chunke Chen, Akshita B. Gopal, Danbi Choi, Timothy J. C. Tan, Yun Sang Tang, Lewis Siu, Armita Nourmohammad, Roberto Bruzzone, Ian A. Wilson, Meng Yuan, Nicholas C. Wu, Chris K. P. Mok   +14 more
wiley   +1 more source

Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies.

PLoS ONE, 2021
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is responsible for the ongoing coronavirus disease (COVID-19) pandemic which is characterized by respiratory illness and severe pneumonia, and currently accounts for > 2.5 million deaths ...
Kaewta Rattanapisit, Christine Joy I Bulaon, Narach Khorattanakulchai, Balamurugan Shanmugaraj, Kittikhun Wangkanont, Waranyoo Phoolcharoen   +5 more
doaj   +1 more source

Iodine value analysis on RBD palm olein and RBD palm stearin

Journal of Midwifery and Nursing
Studies and analyses were made to determine the degree of uncertainty in the iodine value estimation of refined, bleached, and deodorized (RBD) palm oil and after fractination (RBD palm olein and RBD palm stearin). At refining process and produce Refined Bleached Deodorized Palm Oil (RBDPO), enter the fractionation process will produce RBD palm olein ...
Lestari, Puji, Yusuf, Faisal, Furqan, Mainal   +2 more
openaire   +2 more sources

Self-Assembled Particles Combining SARS-CoV-2 RBD Protein and RBD DNA Vaccine Induce Synergistic Enhancement of the Humoral Response in Mice

, 2022
Despite the fact that a range of vaccines against COVID-19 have already been created and are used for mass vaccination, the development of effective, safe, technological, and affordable vaccines continues.
Ekaterina A. Volosnikova, Iuliia A. Merkuleva, Anna V. Zaykovskaya, Lyubov A. Orlova, Oleg V. Pyankov, Boris N. Zaitsev, Alexey M. Zadorozhny, Anastasiya A. Isaeva, Andrey P. Rudometov, Ekaterina V. Starostina, Dmitry N. Shcherbakov, Valentina S. Nesmeyanova, Elena D. Danilenko, Natalya V. Volkova, Alexander V. Taranin, Mariya B. Borgoyakova, Sergei I. Bazhan, Daniil V. Shanshin, Konstantin O. Baranov, Alexander A. Ilyichev, Larisa I. Karpenko   +20 more
core   +1 more source

Covalently Linked Peptides and Membrane Potential Enable CyaA Segment Translocation

Advanced Science, EarlyView.
Using a newly developed Droplet Interface Bilayer‐Pipette assay, the authors show that two segments of the CyaA toxin cross membranes by distinct mechanisms. One segment translocates spontaneously, whereas the translocation of the other depends on membrane potential.
Gaia Scilironi, Nicolas Carvalho, Jacinthe Frangieh, Corentin Léger, Dorothée Raoux‐Barbot, J. Iñaki Guijarro, Daniel Ladant, Sophie Cribier, Nicolas Rodriguez, Alexandre Chenal   +9 more
wiley   +1 more source

Drone Monitoring of Breeding Waterbird Populations: The Case of the Glossy Ibis

Drones, 2018
Waterbird communities are potential indicators of ecological changes in threatened wetland ecosystems and consequently, a potential object of ecological monitoring programs.
Isabel Afán, Manuel Máñez, Ricardo Díaz-Delgado   +2 more
doaj   +1 more source