Coronavirus SARS-CoV-2: Analysis of subgenomic mRNA transcription, 3CLpro and PL2pro protease cleavage sites and protein synthesis [PDF]
arXiv, 2020 Coronaviruses have recently caused world-wide severe outbreaks: SARS (Severe Acute Respiratory Syndrome) in 2002 and MERS (Middle-East Respiratory Syndrome) in 2012. At the end of 2019, a new coronavirus outbreak appeared in Wuhan (China) seafood market as first focus of infection, becoming a pandemics in 2020, spreading mainly into Europe and Asia ...
arxiv
Systemic Expression of a Viral RdRP Protects against Retrovirus Infection and Disease
Journal of Virology, 2020 Immune control of retroviruses is notoriously difficult, a fundamental problem that has been most clinically consequential with the HIV-1 pandemic. As humans expand further into previously uninhabited areas, the likelihood of new zoonotic retroviral exposures increases.
James H. Morrison +6 more
openaire +4 more sources
iMetaOmics, EarlyView.This study focuses on porcine epidemic diarrhea virus (PEDV), a highly virulent and rapidly evolving coronavirus threatening global swine production. We established a novel genotype system of PEDV targeting site‐specific polymorphisms in the spike (S) protein.
Mingkai Lei +10 more
wiley +1 more source
Searching inhibitors for three important proteins of COVID-19 through molecular docking studies [PDF]
arXiv, 2020 The lack of recommended drugs or vaccines to deal with the COVID-19 is the main concern of this pandemic. The approved drugs for similar health problems, drugs under clinical trials, and molecules from medicinal plants extracts are investigated randomly to deal with the COVID-19 infection.
arxiv
Viroinformatics-based investigation of SARS-CoV-2 core proteins for potential therapeutic targets [PDF]
arXiv, 2020 Due to SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) being a novel virus, there are currently no known effective antiviral drugs capable of slowing its progress. To accelerate the discovery of potential drug candidates, bioinformatics based in silico drug discovery can be applied as a very robust tool.
arxiv
Structural Dynamics of Picornaviral RdRP Complexes. Implications for the Design of Antivirals
, 2011 Genome replication in picornavirus is catalyzed by a virally encoded RNA dependent RNA polymerase, termed 3D. These viruses also use a small protein primer, named VPg to initiate RNA replication. Polymerase 3D also catalyzes the covalent linkage of UMP to a N-terminal tyrosine on VPg.
Verdaguer, Núria +2 more
openaire +3 more sources
Molecular docking and binding mode analysis of selected FDA approved drugs against COVID-19 selected key protein targets: An effort towards drug repurposing to identify the combination therapy to combat COVID-19 [PDF]
arXiv, 2020 The emergence of COVID-19 has severely compromised the arsenal of antiviral and antibiotic drugs. Drug discovery is a multistep process with a high failure rate, high cost and it takes approximately 10-12 years for the development of new molecules into the clinical candidate. On the other side, drug repurposing also called old drugs for new uses, is an
arxiv
Ebola Virus L Polymerase RdRp Sequence and Phylogenetic Analysis [PDF]
Egyptian Journal of Biomedical Engineering and Biophysics, 2019 Ebola Virus (EBOV) infection affects humans beings in the last four decades with the deadliest outbreak at 2015 in West Africa, leaving more than 10,000 deaths. The virus harms the liver of the patient when direct contact with contaminated body fluids or blood is occurred.
Amr Ali Desouky +3 more
openaire +2 more sources
Molecular Evolutionary Analyses of the RNA-Dependent RNA Polymerase Region in Norovirus Genogroup II
Frontiers in Microbiology, 2018 Noroviruses are the leading cause of viral gastroenteritis in humans across the world. RNA-dependent RNA polymerase (RdRp) plays a critical role in the replication of the viral genome.
Keita Ozaki +9 more
doaj +1 more source
Crystallisation of Zika NS5 RdRp v1
The main aim of this work was to identify small molecules that bind Zika NS5 RdRp (catalytic RNA-dependent RNA polymerase domain) through X-ray fragment-based screening. The Zika NS5 RDRP domain was cloned, expressed, purified, and crystallised. Suitable crystals for fragment screening were produced and optimised allowing an extensive fragment ...
Anu V. Chandran +2 more
openaire +1 more source