Results 161 to 170 of about 1,607,122 (265)

Mining Thermophile Photosynthesis Genes: A Synthetic Operon Expressing <i>Chloroflexota</i> Species Reaction Center Genes in <i>Rhodobacter sphaeroides</i>. [PDF]

Biomolecules
Rehman Y, Kim Y, Tong M, Blaby IK, Blaby-Haas CE, Beatty JT.   +5 more
europepmc   +1 more source

X-ray structure of the Rhodobacter sphaeroides reaction center with an M197 Phe→His substitution clarifies the properties of the mutant complex. [PDF]

IUCrJ, 2022
Selikhanov G, Fufina T, Guenther S, Meents A, Gabdulkhakov A, Vasilieva L.   +5 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Tunneling Mechanisms of Quinones in Photosynthetic Reaction Center-Light Harvesting 1 Supercomplexes. [PDF]

Small Sci
Mao R, Guo J, Bie L, Liu LN, Gao J.
europepmc   +1 more source

Electronic Structure Effects Related to the Origin of the Remarkable Near-Infrared Absorption of Blastochloris viridis' Light Harvesting 1-Reaction Center Complex. [PDF]

J Chem Theory Comput, 2022
Mondragón-Solórzano G, Sandoval-Lira J, Nochebuena J, Nochebuena J, Cisneros GA, Barroso-Flores J.   +5 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

The Quenching of Long-Wavelength Fluorescence by the Closed Reaction Center in Photosystem I in Thermostichus vulcanus at 77 K. [PDF]

Int J Mol Sci
Akhtar P, van Stokkum IHM, Lambrev PH.
europepmc   +1 more source

Excitonic structure and charge separation in the heliobacterial reaction center probed by multispectral multidimensional spectroscopy. [PDF]

Nat Commun, 2021
Song Y, Sechrist R, Nguyen HH, Johnson W, Abramavicius D, Redding KE, Ogilvie JP.   +6 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Simulations predict preferred Mg²⁺ coordination in a nonenzymatic primer-extension reaction center. [PDF]

Biophys J
Mittal S, Nisler C, Szostak JW.
europepmc   +1 more source