Results 1 to 10 of about 1,529,317 (170)
Simulation of between repeat variability in real time PCR reactions. [PDF]
PLoS ONE, 2012 While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process.
Antoon Lievens +3 more
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BioTechniques, 2008 AbstractReal‐time PCR is a recent modification to the polymerase chain reaction that allows precise quantification of specific nucleic acids in a complex mixture by fluorescent detection of labeled PCR products. Detection can be accomplished using specific, as well as nonspecific fluorescent probes.
Lynne Lederman
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Real-time PCR for mRNA quantitation
BioTechniques, 2005 Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to ...
Marisa L. Wong, Juan F. Medrano
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Frontiers in Microbiology, 2021 The objectives of this study were to evaluate the prevalence of extended spectrum β-lactamase (ESBL) genes, AmpC-type β-lactamase (ACBL) genes, and plasmid mediated quinolone resistance (PMQR) genes in Salmonella isolated at a Veterinary Medical Teaching
Carl Basbas +6 more
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Frontiers in Molecular Biosciences, 2023 Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Although many therapeutic options are available, several factors, including the presence of p53 mutations, impact tumor development and therapeutic resistance ...
Mariana M. Paz +10 more
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Scientific Reports, 2022 In the heart tissue of acutely Trypanosoma cruzi-infected mice miR-145-5p and miR-146b-5p are, respectively, downregulated and upregulated. Here, we used the H9C2 rat cardiomyoblast cell line infected with the Colombian T. cruzi strain to investigate the
Priscila Silva Grijó Farani +4 more
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Monitoring for freshwater mussel presence in rivers using environmental DNA
Environmental DNA, 2021 In 2013, the U.S. Environmental Protection Agency (USEPA) updated its national recommended water quality criteria for the protection of aquatic life from the toxic effects of ammonia in freshwaters.
Ellen P. Preece +4 more
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Frontiers in Cellular and Infection Microbiology, 2021 Chronic Chagas cardiomyopathy (CCC) is the most frequent and severe form of Chagas disease, a neglected tropical illness caused by the protozoan Trypanosoma cruzi, and the main cause of morbimortality from cardiovascular problems in endemic areas ...
Priscila Silva Grijó Farani +8 more
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Development of a Two Triplex Real-Time Polymerase Chain Reaction for Rapid Detection of Six Carbapenemase Genes in Enterobacteriaceae [PDF]
Osong Public Health and Research Perspectives, 2020 Objectives Carbapenem resistance is a serious clinical and public health threat. Carbapenemase can confer carbapenem resistance, and most carbapenemase genes are plasmid encoded so resistance can easily spread.
Ji Ae Choi +3 more
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Life, 2023 In the absence of validated biomarkers to control the cure of Chagas disease, PCR-based diagnosis is being used as the main tool for an early indication of therapeutic failure.
Otacilio C. Moreira +12 more
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