Results 31 to 40 of about 1,529,317 (170)

Digital PCR as a tool to measure HIV persistence [PDF]

open access: yes, 2018
Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped.
Bosman, Kobus   +4 more
core   +1 more source

Statistical analysis of real-time PCR data

open access: yesBMC Bioinformatics, 2006
Background Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR are still lacking; specifically in the realm of appropriate statistical treatment.
Reed Ann   +3 more
doaj   +1 more source

Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays [PDF]

open access: yes, 2018
Background Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infection. The development of automated DNA extraction methodologies and PCR systems is an important step toward the standardization of protocols ...
Abras, Alba   +12 more
core   +3 more sources

Effect of adjunctive amoxicillin/metronidazole treatment on subgingival Tannerella forsythia, Prevotella intermedia and Fusobacterium nucleatum recolonization in periodontitis patients

open access: yesSelcuk Dental Journal, 2019
Background: The use of antibiotics adjunctive to non-surgical periodontal treatment is an accepted approach in the treatment of periodontitis. However, bacterial recolonization is a major drawback which complicates the maintenance of therapeutic ...
Emre Yaprak   +2 more
doaj   +1 more source

Multiple internal controls enhance reliability for PCR and real time PCR detection of Rathayibacter toxicus

open access: yesScientific Reports, 2021
Rathayibacter toxicus is a toxigenic bacterial plant pathogen indigenous to Australia and South Africa. A threat to livestock industries globally, the bacterium was designated a U.S. Select Agent.
Mohammad Arif   +4 more
doaj   +1 more source

Use of real-time polymerase chain reaction to identify Entamoeba histolytica in schoolchildren from northwest Mexico

open access: yesJournal of Infection in Developing Countries, 2017
Introduction: Entamoeba histolytica, E. dispar, and E. moshkovskii are morphologically identical, but intestinal amebiasis is caused only by E. histolytica.
Sandra Aguayo-Patrón   +3 more
doaj   +1 more source

Syndromic surveillance and pathogen detection using multiplex assays for respiratory infections in small ruminants [PDF]

open access: yes, 2018
Background Several bacterial and viruses can infect the respiratory tract of small ruminants causing similar clinical signs. The differential diagnosis of respiratory diseases in small ruminants can be achieved using multi-plex assays for an accurate ...
Cattoli, Giovanni   +5 more
core  

Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus [PDF]

open access: yes, 2007
The pinewood nematode (PWN), Bursaphelenchus xylophilus , is a major pathogen of conifers, which impacts on forest health, natural ecosystem stability and international trade. As a consequence, it has been listed as a quarantine organism in Europe.
BOONHAM, NEIL   +9 more
core   +1 more source

Quantitative analysis of the dystrophin gene by real-time PCR [PDF]

open access: yes, 2012
Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine ...
Anđelkovic Ana   +12 more
core   +2 more sources

Virus Culture and Real-time RT-PCR in Identifying Influenza Viruses From Influenza-like Illness Cases in Indonesia 2007-2008 [PDF]

open access: yes, 2011
Introduction: From the influenza-like illness (ILI) surveillance in Indonesia, we learned that there was disagreement between virus culture and reverse trancriptase polymerase chain reaction (RT-PCR).
Indalao, I. L. (Irene)   +3 more
core  

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