Results 41 to 50 of about 1,108,560 (151)

Agreement between two real-time commercial PCR kits and an in-house real-time PCR for diagnosis of mucormycosis

Microbiology Spectrum
Mucormycosis is a severe and emerging invasive fungal infection associated with high mortality rates. Early diagnosis is crucial for initiating specific antifungal treatment, with molecular tools currently representing the most efficient diagnostic ...
Lovanirina Clémencia Rafanomezantsoa, Estelle Sabourin, Nadia Guennouni Sebbouh, Emilie Sitterlé, Nada Ben Halima, Yannick Sonjah Raveloarisaona, Gilles Quesne, Eric Dannaoui, Marie-Elisabeth Bougnoux   +8 more
doaj   +1 more source

Real-time PCR applications for diagnosis of leishmaniasis

Parasites & Vectors, 2018
Leishmaniasis is a vector-borne disease caused by many Leishmania species, which can infect both humans and other mammals. Leishmaniasis is a complex disease, with heterogeneous clinical manifestations ranging from asymptomatic infections to lesions at ...
Luca Galluzzi, Marcello Ceccarelli, Aurora Diotallevi, Michele Menotta, Mauro Magnani   +4 more
doaj   +1 more source

Malaria real-time PCR: correlation with clinical presentation

New Microbes and New Infections, 2015
Among 112 patients infected only by Plasmodium falciparum, WHO criteria of severity were compared with parasite load assessed by microscopy and quantitative PCR.
L. Dormond, K. Jaton, S. de Vallière, B. Genton, G. Greub   +4 more
doaj   +1 more source

Optimization of Human Cytomegalovirus LightCycler Real-Time PCR

Journal of Infection in Developing Countries, 2008
Background: Real-time PCR has been widely considered as a powerful tool for the evaluation of Human Cytomegalovirus (CMV) DNA kinetics. Successful PCR relies on optimization, which is an extremely demanding procedure.
Wafa Habbal, Fawza Monem, Barbara Christine Gärtner   +2 more
doaj   +1 more source

Troubleshooting Real-Time PCR Screening for Leishmania infantum

Journal of Arthropod-Borne Diseases, 2018
Troubleshooting Real-Time PCR Screening for Leishmania ...
Philippe Gil de MENDONCA
doaj   +1 more source

Transcriptional Changes in Response to Arbuscular Mycorrhiza Development in the Model Plant Medicago truncatula

Molecular Plant-Microbe Interactions, 2003
Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf, Katja Manthey, Jasmin Doll, Andreas M. Perlick, Burkhard Linke, Thomas Bekel, Folker Meyer, Philipp Franken, Helge Küster, Franziska Krajinski   +9 more
doaj   +1 more source

Real-time PCR: Concept and Application in Livestock

International Journal of Bio-Resource and Stress Management, 2023
Real-time PCR, also called quantitative PCR or RT-qPCR, is a technique which provide a simple and elegant method for determining the amount of a target sequence or gene that is present in a sample. It is a gold standard method for accurate and sensitive
Mahesh S. Dige, L. S. Kokate, B. Inamdar, Ananta Kumar Das   +3 more
doaj  

Real-Time PCR Assay for Quantitative Mismatch Detection

BioTechniques, 2003
We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively, L. Chang, J.M. LeBon, Q. Liu, A.D. Riggs, J. Singer-Sam   +5 more
doaj   +1 more source

Molecular Analysis of Host Immune Responses to the Resident Colonic Microflora [PDF]

Iranian Journal of Public Health, 2005
Ulcerative colitis (UC) is an idiopathic inflammatory bowel disease that affects the colonic mucosa. UC results from loss of tolerance towards the normal gut microbiota, leading to chronic mucosal inflammation.
B Bahrami, GT Macfarlane
doaj  

Real-time PCR v1

, 2017

  +4 more sources