Results 41 to 50 of about 1,529,317 (170)

Development of rapid, automated diagnostics for infectious disease: advances and challenges [PDF]

open access: yes, 2009
The last 2 years has seen an exponential rise in the amount of research funding made available for the development of rapid diagnostic devices for infectious agents of medical importance. This review reports on several such projects.
Ince, J, McNally, A
core   +1 more source

Rapid Identification of Pseudomonas aeruginosa by Using Real Time PCR

open access: yesKufa Journal for Veterinary Medical Sciences, 2016
Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens.To characterizes P.
Marrib N. Rasheed   +2 more
doaj   +1 more source

On joint maximum-likelihood estimation of PCR efficiency and initial amount of target [PDF]

open access: yes, 2007
We consider the problem of estimating unknown parameters of the real-time polymerase chain reaction (RTPCR) from noisy observations. The joint ML estimator of the RT-PCR efficiency and the initial number of DNA target molecules is derived.
Hassibi, A., Hassibi, B., Vikalo, H.
core   +1 more source

The application of PCR based methods in diagnostics of some viral infections of swine [PDF]

open access: yesBiotechnology in Animal Husbandry, 2016
Viral infections of swine cause significant economic losses in swine husbandry. They manifest in death of infected animals of different ages or in decreased productivity during the manufacturing process.
Nišavić Jakov   +4 more
doaj  

Multicolor combinatorial probe coding for real-time PCR. [PDF]

open access: yesPLoS ONE, 2011
The target volume of multiplex real-time PCR assays is limited by the number of fluorescent dyes available and the number of fluorescence acquisition channels present in the PCR instrument.
Qiuying Huang   +8 more
doaj   +1 more source

Strangles: The Molecular Identification and Epidemiology of Streptococcus equi subsp. equi [PDF]

open access: yes, 2012
A conventional PCR diagnostic test was established to confirm the microbiological isolation of Streptococcus equi subsp. equi (S. equi), the causative agent of strangles.
Patty, Olivia Anne
core   +1 more source

A cost effective real-time PCR for the detection of adenovirus from viral swabs [PDF]

open access: yes, 2013
Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories.
Jason J LeBlanc   +7 more
core   +1 more source

A simple and cost-effective real-time PCR method using diluted and heat-treated whole blood lysate

open access: yesScientific Reports
Although DNA extraction is a crucial step before polymerase chain reaction (PCR), attempts to perform PCR without DNA isolation have been ongoing since the 1990s.
Gökçe Güllü Amuran   +3 more
doaj   +1 more source

Development and verification of PCR based assay to dectect and quantify garden pea lec gene

open access: yesCzech Journal of Food Sciences, 2012
Genetically modified organisms (GMOs) entering the food chain have became its part, which is necessary to monitor. GMO analyses are used as a control mechanism according to valid acquis communautaire for traceability and labeling of GMOs.
Aleš VRÁBLÍK   +4 more
doaj   +1 more source

Detection of Panax notoginseng and its products using a duplex quantitative polymerase chain reaction assay

open access: yesJournal of Agriculture and Food Research, 2023
Panax notoginseng is a traditional Chinese medicinal herb. With its wide applications in medicine, food products, health care, cosmetics and other industries, identification and detection of P. notoginseng have become critical.
Chao Ji   +9 more
doaj   +1 more source

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