A real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes [PDF]
, 2006 We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting Wuchereria bancrofti DNA in human blood and in mosquitoes. An assay based on detection of the W.
Atkinson, Laura J +9 more
core +2 more sources
Effects of Flight on Gene Expression and Aging in the Honey Bee Brain and Flight Muscle [PDF]
, 2012 Honey bees move through a series of in-hive tasks (e.g., “nursing”) to outside tasks (e.g., “foraging”) that are coincident with physiological changes and higher levels of metabolic activity.
Ammons, Andrew +5 more
core +5 more sources
Evaluation of VERSANT® CT/GC DNA 1.0 Assay in conjunction with VERSANT® kPCR Molecular system
Microbiologia Medica, 2011 A total of 463 first void urine specimens were obtained from patients attending the Sexually Transmitted Diseases (STD) Outpatients Clinic of the St. Orsola University Hospital in Bologna, Italy. Samples were tested by the new test VERSANT® CT/GC DNA 1.0
Antonella Marangoni +9 more
doaj +1 more source
A prospective observational study of mycophenolate mofetil treatment in progressive diffuse cutaneous systemic sclerosis of recent onset. [PDF]
, 2012 OBJECTIVE: A prospective observational study of mycophenolate mofetil (MMF) treatment in patients with diffuse progressive cutaneous systemic sclerosis (SSc) of recent onset.
Jimenez, Sergio A. +3 more
core +3 more sources
Detection of Leishmania infantum in Lutzomyia longipalpis captured in Campo Grande, MS
Revista Brasileira de Parasitologia VeterináriaLeishmaniasis is a zoonotic disease caused by protozoa of the genus Leishmania (Ross, 1903) and is the focus of considerable attention in human and veterinary medicine.
Rodrigo Casquero Cunha +3 more
doaj +1 more source
Alexandria Journal of Medicine, 2016 Onychomycosis is the most common nail disorder, accounting for up to 50% of all nail problems and about 30% of cutaneous fungal infections. Treatment of onychomycosis is expensive.
Sherin M. Emam, Osama H. Abd El-salam
doaj +1 more source
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions [PDF]
, 2003 BACKGROUND: Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development of a ...
Bell, S.M. +13 more
core +3 more sources
Ciência Rural, 2012 O objetivo deste trabalho foi realizar a validação de uma reação em cadeia da polimerase em tempo real com o sistema Plexor® (qPCR) para o diagnóstico da Leucose Enzoótica Bovina (LEB), por meio da comparação com testes de diagnóstico recomendados pela ...
Natanael Lamas Dias +3 more
doaj
Effects of Chiral Gold Nanoflowers on Polymerase Chain Reaction
Nano Biomedicine and Engineering, 2011 In this paper, we reported that chiral gold nanoflowers with the concentration of 0.08 mM can enhance the specificity of polymerase chain reaction (PCR) and eliminate unspecific products, and the efficiency of PCR declines in some extent and the melt ...
Xiao Zhi +3 more
doaj +1 more source
Fetal Sex and RHD Genotyping with Digital PCR Demonstrates Greater Sensitivity than Real-time PCR. [PDF]
, 2015 BACKGROUND: Noninvasive genotyping of fetal RHD (Rh blood group, D antigen) can prevent the unnecessary administration of prophylactic anti-D to women carrying RHD-negative fetuses. We evaluated laboratory methods for such genotyping.
Avent, ND +7 more
core +2 more sources