Malaria real-time PCR: correlation with clinical presentation
New Microbes and New Infections, 2015 Among 112 patients infected only by Plasmodium falciparum, WHO criteria of severity were compared with parasite load assessed by microscopy and quantitative PCR.
L. Dormond +4 more
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Investigations into the utility of real-time PCR for the detection, quantitation and characterisation of clinically relevant viruses [PDF]
, 2003 The use of PCR as a tool for the diagnostic virology and viral research laboratories has greatly increased in recent years, however the use of conventional PCR and amplicon detection systems can be a complex and relatively slow process that increases the
Mackay, Ian M.
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Optimization of Human Cytomegalovirus LightCycler Real-Time PCR
Journal of Infection in Developing Countries, 2008 Background: Real-time PCR has been widely considered as a powerful tool for the evaluation of Human Cytomegalovirus (CMV) DNA kinetics. Successful PCR relies on optimization, which is an extremely demanding procedure.
Wafa Habbal +2 more
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Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples [PDF]
, 2005 Aims: To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. Methods and results: The specificity of a developed 5’nuclease rt-PCR for
Al Rashid S.T. +10 more
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Methods of allergen detection based on DNA analysis [PDF]
, 2011 Many allergens, such as hazelnut, peanut, charlock, celery, sesame, lupine, walnut, almond, macadamia nut, hickory, pistachio, wheat gliadins, may be present in food products, however, undeclared or as unintentional additives.
Majak, Iwona, Słowianek, Marta
core
Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize [PDF]
, 2012 Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a
Atoui, Ali +3 more
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A novel and universal method for microRNA RT-qPCR data normalization [PDF]
, 2009 Gene expression analysis of microRNA molecules is becoming increasingly important. In this study we assess the use of the mean expression value of all expressed microRNAs in a given sample as a normalization factor for microRNA real-time quantitative PCR
De Weer, An +6 more
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Troubleshooting Real-Time PCR Screening for Leishmania infantum
Journal of Arthropod-Borne Diseases, 2018 Troubleshooting Real-Time PCR Screening for Leishmania ...
Philippe Gil de MENDONCA
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Real-time PCR: Concept and Application in Livestock
International Journal of Bio-Resource and Stress Management, 2023 Real-time PCR, also called quantitative PCR or RT-qPCR, is a technique which provide a simple and elegant method for determining the amount of a target sequence or gene that is present in a sample. It is a gold standard method for accurate and sensitive
Mahesh S. Dige +3 more
doaj
Molecular Plant-Microbe Interactions, 2003 Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf +9 more
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