Results 51 to 60 of about 637,530 (302)

Data representing applicability of developed growth hormone 1 (GH1) gene detection method for detecting Atlantic salmon (Salmo salar) at high specificity to processed salmon commodities

Data in Brief, 2019
This article is referred to the research article entitled “Development of a novel method for specific detection of genetically modified Atlantic salmon, AquAdvantage, using real-time polymerase chain reaction” by Soga et al.
Keisuke Soga, Kosuke Nakamura, Takumi Ishigaki, Shinya Kimata, Kiyomi Ohmori, Masahiro Kishine, Junichi Mano, Reona Takabatake, Kazumi Kitta, Hiroyuki Nagoya, Kazunari Kondo   +10 more
doaj   +1 more source

Novel Approaches to the Identification of Streptococcus pneumoniae as the Cause of Community-Acquired Pneumonia [PDF]

, 2017
Current diagnostic tests lack sensitivity for the identification of the bacterial etiology of pneumonia. Attempts during the past 2 decades to improve sensitivity of detection of bacterial constituents in blood by use of antibody-antigen complexes and ...
Albrich, Werner C., Klugman, Keith P., Madhi, Shabir A.   +2 more
core  

Molecular characterisation of human penile carcinoma and generation of paired epithelial primary cell lines

Molecular Oncology, EarlyView.
Generation of two normal and tumour (cancerous) paired human cell lines using an established tissue culture technique and their characterisation is described. Cell lines were characterised at cellular, protein, chromosome and gene expression levels and for HPV status.
Simon Broad, Mahmood Hachim, Tom Bertin, Karolina Penderecka, Rifat Hamoudi, Saif Khan, Rui Henrique, Natalie Bergmoser, Manit Arya, Kalle Sipila, Matteo Vietri Rudan, Asif Muneer, Aamir Ahmed   +12 more
wiley   +1 more source

REAL TIME POLYMERASE CHAIN REACTION IN TULAREMIA LABORATORY DIAGNOSTICS

Журнал микробиологии, эпидемиологии и иммунобиологии, 2015
Aim. Enhancement of tularemia laboratory diagnostics by F. tularensis DNA determination in blood sera of patients using real time polymerase chain reaction (RT-PCR). Materials and methods.
M. I Kormilitsyna, I. S Mescheryakova, T. V Mikhailova, A. A Dobrovolsky   +3 more
doaj  

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Emerging Infectious Diseases, 2004
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV).
Shannon L. Emery, Dean D. Erdman, Michael D. Bowen, Bruce R. Newton, Jonas M. Winchell, Richard F. Meyer, Suxiang Tong, Byron T. Cook, Brian P. Holloway, Karen A. McCaustland, Paul A. Rota, Bettina Bankamp, Luis E. Lowe, Tom G. Ksiazek, William J. Bellini, Larry J. Anderson   +15 more
doaj   +1 more source

ATF4‐mediated stress response as a therapeutic vulnerability in chordoma

Molecular Oncology, EarlyView.
We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone, James Dunford, Eleanor Calcutt, Vicki Gamble, Filiz Senbabaoglu Aksu, Lorena Ligammari, Georgina Wherry, Giorgia Gaeta, John C. Christianson, Adrienne M. Flanagan, Udo Oppermann, Adam P. Cribbs   +11 more
wiley   +1 more source

Infrequent Replication of Parvovirus B19 and Erythrovirus Genotypes 2 and 3 among HIV-Infected Patients with Chronic Anemia [PDF]

, 2017
We investigated the role that erythroviruses (parvovirus B19 and erythrovirus genotypes 2 and 3) play in the lives of immunosuppressed HIV-infected patients with chronic anemia.
Bernasconi, Enos, Calmy, Alexandra, Dang, Thanh, Delhumeau, Cécile, Elzi, Luigia, Ferry, Tristan, Hirschel, Bernard, Meylan, Pascal, Rauch, Andri, Schmid, Patrick, Weber, Rainer   +10 more
core  

Transcriptional regulation of neuropeptide receptors underlies context‐dependent adaptation in Drosophila melanogaster

FEBS Open Bio, EarlyView.
Under environmental changes, the expression level of neuropeptide (NP) and neuropeptide receptor (NPR) genes changes to confer context‐dependent adaptation to the model organism Drosophila melanogaster. Through finding more regulatory elements in the NPR genes in comparison with their ligands (NPs), we found that NPR‐biased transcriptional regulation ...
SeungHeui Ryu, Yanan Wei, Zekun Wu, Tianmu Zhang, DoHoon Lee, Hadi Najafi, Woo Jae Kim   +6 more
wiley   +1 more source

Establishment and optimization of the two‐step induction system for generating primordial germ cell‐like cells from chicken embryonic stem cells

FEBS Open Bio, EarlyView.
This study optimizes a two‐step induction system to generate PGCLCs from chicken ESCs. The induced cells express germline markers and migrate to gonads in ovo, offering a novel approach for avian genetic resource conservation. Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs.
Zeyu Li, XianShuai Xu, GuangZheng Liu, XiaoQian Lv, JiuZhou Song, HongYan Sun, YingJie Niu, QiSheng Zuo, Wei Han, BiChun Li, Kai Jin   +10 more
wiley   +1 more source