Results 71 to 80 of about 734,685 (383)

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Emerging Infectious Diseases, 2004
A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV).
Shannon L. Emery, Dean D. Erdman, Michael D. Bowen, Bruce R. Newton, Jonas M. Winchell, Richard F. Meyer, Suxiang Tong, Byron T. Cook, Brian P. Holloway, Karen A. McCaustland, Paul A. Rota, Bettina Bankamp, Luis E. Lowe, Tom G. Ksiazek, William J. Bellini, Larry J. Anderson   +15 more
doaj   +1 more source

TaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola, the fungus associated with snake fungal disease

BMC Veterinary Research, 2015
BackgroundFungal skin infections associated with Ophidiomyces ophiodiicola, a member of the Chrysosporium anamorph of Nannizziopsis vriesii (CANV) complex, have been linked to an increasing number of cases of snake fungal disease (SFD) in captive snakes ...
Elizabeth A. Bohuski, J. Lorch, Kathryn M Griffin, David S. Blehert   +3 more
semanticscholar   +1 more source

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]

, 2019
Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A., Caruso A. G., Davino S., Panno S., Ruiz-Ruiz S., San Ambrosio M. I. F.   +5 more
core   +1 more source

Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells

Molecular Oncology, EarlyView.
Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, Gökhan Karakülah, Serif Senturk   +11 more
wiley   +1 more source

Multiplexed Real-Time Polymerase Chain Reaction on a Digital Microfluidic Platform [PDF]

Analytical Chemistry, 2010
This paper details the development of a digital microfluidic platform for multiplexed real-time polymerase chain reactions (PCR). Liquid samples in discrete droplet format are programmably manipulated upon an electrode array by the use of electrowetting.
Allen E. Eckhardt, Zhishan Hua, Michael G. Pollack, Jonathan L. Benton, Thomas G. Mitchell, Wiley A. Schell, Vijay Srinivasan, Jeremy Rouse, Vamsee K. Pamula   +8 more
openaire   +3 more sources

Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool.

American Journal of Tropical Medicine and Hygiene, 2017
Fasciola hepatica is the most widely distributed trematode infection in the world. Control efforts may be hindered by the lack of diagnostic capacity especially in remote endemic areas. Polymerase chain reaction (PCR)-based methods offer high sensitivity
M. Cabada, M. Cabada, J. Malaga, J. Malaga, A. Castellanos-Gonzalez, Kelli A. Bagwell, Patrick A Naeger, Hayley K. Rogers, Safa Maharsi, M. Mbaka, A. White   +10 more
semanticscholar   +1 more source

Targeting the AKT/mTOR pathway attenuates the metastatic potential of colorectal carcinoma circulating tumor cells in a murine xenotransplantation model

Molecular Oncology, EarlyView.
Dual targeting of AKT and mTOR using MK2206 and RAD001 reduces tumor burden in an intracardiac colon cancer circulating tumor cell xenotransplantation model. Analysis of AKT isoform‐specific knockdowns in CTC‐MCC‐41 reveals differentially regulated proteins and phospho‐proteins by liquid chromatography coupled mass spectrometry. Circulating tumor cells
Daniel J. Smit, Thais Pereira‐Veiga, Helena Brauer, Michael Horn, Paula Nissen, Thomas Mair, Bente Siebels, Hannah Voß, Ruimeng Zhuang, Marie‐Therese Haider, Desiree Loreth, Margarita Iskhakova, Bele Lindemann, Julian Kött, Laure Cayrefourcq, Jasmin Wellbrock, Hartmut Schlüter, Klaus Pantel, Catherine Alix‐Panabières, Manfred Jücker   +19 more
wiley   +1 more source

Real-Time Polymerase chain reaction trends in COVID-19 patients

Pakistan Journal of Medical Sciences, 2020
Objective: To assess trends of real-time Polymerase Chain Reaction test in Coronavirus infected Patients. Methods: This cross-sectional analytical study was conducted at Tertiary Care Institute, Rawalpindi from March 2020 to June 2020. All patients confirmed COVID positive by real-time Polymerase Chain Reaction (PCR) with recent travel history ...
Sana Abbas, Beenish Abbas, Rashid Iqbal, Aisha Rafique   +3 more
openaire   +4 more sources

A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations.

American Journal of Tropical Medicine and Hygiene, 2013
Diagnosis of gastrointestinal parasites has traditionally relied on stool microscopy, which has low diagnostic sensitivity and specificity. We have developed a novel, rapid, high-throughput quantitative multi-parallel real-time polymerase chain reaction (
Rojelio Mejia, Yosselin Vicuña, Nely Broncano, C. Sandoval, M. Vaca, M. Chico, P. Cooper, T. Nutman   +7 more
semanticscholar   +1 more source

ERM®-AD623: Certified Reference Material for the Quantification of BCR-ABL1 Transcripts [PDF]

, 2015
The JRC’s Institute for Reference Materials and Measurements (IRMM) has released the certified reference material (CRM) ERM-AD623 for the calibration of quantitative real time polymerase chain reaction (qPCR) assays measuring the BCR-ABL1 transcript ...
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