Results 251 to 260 of about 22,071,005 (304)

Programmable Paracrine‐Mimetic Microneedle System for Temporal Regulation of Cardiac Repair

Advanced Science, EarlyView.
Temporal signaling is encoded into a programmable paracrine‐mimetic microneedle patch via tris(2,2'‐bipyridyl)dichlororuthenium(II) hexahydrate/sodium persulfate‐tuned hydrogel microparticles that deliver TGF‐β, IGF‐1, and VEGF over phase‐matched 7‐, 14‐, and 28‐day windows.
Yichen Dai, Pengchong Du, Zhaoyang Wang, Surilige, Rangga Fokta, Renjian Tan, Xiqing Zhao, Shuoyi Zhang, Yuecheng Wang, Yixin Zhang, Ziliang Fu, Rui Gong, Cheng Jiang, Yisheng He, Qiguang Wang, Renke Li, Khoon Lim, Junnan Tang, Xiaolin Cui   +18 more
wiley   +1 more source

Toward Material‐Specific Peptides From in Silico Guided Peptide Engineering to Target PET in the Presence of PS

Advanced Science, EarlyView.
This work presents a computational–experimental strategy to engineer material‐binding peptides toward polymer specificity. Molecular dynamics simulations identified residues driving polystyrene binding, enabling targeted substitutions that greatly reduced polystyrene affinity while preserving PET binding.
Julian Luka, Marian Bienstein, Jonas Berger, Ulrich Schwaneberg   +3 more
wiley   +1 more source

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Basic principles of real-time quantitative PCR

Expert Review of Molecular Diagnostics, 2005
Real-time quantitative PCR allows the sensitive, specific and reproducible quantitation of nucleic acids. Since its introduction, real-time quantitative PCR has revolutionized the field of molecular diagnostics and the technique is being used in a rapidly expanding number of applications.
Manit, Arya, Iqbal S, Shergill, Magali, Williamson, Lyndon, Gommersall, Neehar, Arya, Hitendra R H, Patel   +5 more
openaire   +4 more sources

Accurate Real-time Reverse Transcription Quantitative PCR

Methods in Molecular Biology, 2009
Within the last few years real-time quantitative PCR has become the method of choice for the accurate quantification of mRNA levels. Compared to previous methods the sensitivity of real-time quantitative PCR improved to the detection limit of up to one single molecule per reaction tube.
Marco, Klatte, Petra, Bauer
openaire   +4 more sources

Real-time quantitative PCR in parasitology

Trends in Parasitology, 2002
Standard techniques for counting parasites are often time-consuming, difficult and inaccurate, and occasionally unpleasant. Real-time quantitative polymerase chain reaction has recently been applied to parasitology, specifically Plasmodium, Toxoplasma, Leishmania and Neospora.
Andrew, Bell, Lisa, Ranford-Cartwright
openaire   +3 more sources

Quantitative detection of periodontopathogens by real-time PCR

Journal of Microbiological Methods, 2004
Specific bacteria are believed to play an important role in chronic periodontitis, yet the significance of their relative numbers in initiation and progress of the disease is still unclear. We report here the development of a sensitive, quantitative PCR technique for enumerating Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg ...
Claudia, Nonnenmacher, Alexander, Dalpke, Reinier, Mutters, Klaus, Heeg   +3 more
openaire   +2 more sources

Accuracy of quantitative real-time PCR analysis

Doklady Biochemistry and Biophysics, 2013
When solving many problems in biology and med icine, it is necessary to detect the presence of specific nucleic acid sequences in samples. For this purpose, polymerase chain reaction (PCR)—a chain enzy matic reaction of multiplication of the analyzed DNA fragment, realized in the form of successive rounds of duplication of fragments present in the ...
D G, Sochivko, A A, Fedorov, D A, Varlamov, V E, Kurochkin, R V, Petrov   +4 more
openaire   +2 more sources

Real-Time Quantitative PCR of Telomere Length

Molecular Biotechnology, 2004
Telomeres cap the ends of chromosomes and are essential for the protection of chromosomes, as well as restricting the replicative potential of a cell. These functions are achieved by the regulation of telomeric repeat length, making the measurement of telomere length a useful aid in the elucidation of the replicative history and potential of cells ...
Marcel E, Gil, Thérèsa L, Coetzer
openaire   +2 more sources

Evaluation of uncertainty in quantitative real-time PCR

Journal of Microbiological Methods, 2006
Quantitative real-time PCR is one of the newer methods for measurement of the amount of nucleic material in biological systems. However, reliable measurement requires an appropriate estimation of uncertainty and this paper has developed the uncertainty budget associated with this procedure using as an example, data from a quantitative real-time PCR ...
John L, Love, Paula, Scholes, Brent, Gilpin, Marion, Savill, Susan, Lin, Laly, Samuel   +5 more
openaire   +2 more sources

Quantitative Real-Time PCR: Recent Advances

2016
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a "closed-tube" system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or ...
Charanjeet, Singh, Sinchita, Roy-Chowdhuri   +1 more
openaire   +2 more sources