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RNA Isolation and Real-Time Quantitative RT-PCR
2008Adipose tissue has emerged as a major endocrine organ producing a wide spectrum of hormones and factors that play crucial roles in regulating cell turnover and function, not only locally within the adipose tissue but also in the brain and other key metabolic organ systems.
Haiyan, Guan, Kaiping, Yang
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Quantitative (Real-Time) RT-PCR in Cardiovascular Research
2007Quantitative (real-time) PCR (qPCR) represents a highly sensitive, sequence-specific, and reproducible technique for the gel-free detection and quantitation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples.
Ashton, KJ, Headrick, JP
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Semi-Quantitative Real-Time PCR for Pain Research
2004In this chapter we cover what we have found to be a "best practice" for real-time polymerase chain reaction (PCR) for relative mRNA quantification. We describe our techniques for tissue-sample collection and freezing, sample handling for quick and reproducible extraction of total RNA, first strand cDNA synthesis, real-time PCR amplification, and ...
Hong Qing, Guo, Sandra R, Chaplan
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Real-Time Quantitative PCR Analysis of Viral Transcription
2004Whole-genome profiling using DNA arrays has led to tremendous advances in our understanding of cell biology. It has had similar success when applied to large viral genomes, such as the herpesviruses. Unfortunately, most DNA arrays still require specialized and expensive resources and, generally, large amounts of input RNA. An alternative approach is to
James, Papin +3 more
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Quantitative Real Time PCR in Plant Developmental Biology
2010Gene expression patterns are important determinants of a cell's state, and changes in the expression profile indicate adaptation processes as a response to developmental transitions or environmental changes. Assaying gene expression can, therefore, help to elucidate mechanisms of determination and differentiation, as well as signaling networks. Several
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Real-Time Quantitative PCR: A Tool for Absolute and Relative Quantification
Biochemistry and Molecular Biology Education, 2021Rex Arunraj
exaly