Results 261 to 270 of about 22,071,005 (304)

High‐Throughput Quantitative Real‐Time PCR

Current Protocols in Human Genetics, 2008
AbstractRecent technical advances in quantitative real‐time PCR (qRT‐PCR) have allowed for extensive miniaturization, thereby rendering the technique amenable to high‐throughput assays. Large numbers of different nucleic acids can now rapidly be measured quantitatively. Many investigations can benefit from this approach, including determination of gene
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Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry, 2008
To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort.The RT-PCR assay for PCA3 is based on target-specific lanthanide probes ...
Riina-Minna, Väänänen, Maria, Rissanen, Otto, Kauko, Siina, Junnila, Ville, Väisänen, Jussi, Nurmi, Kalle, Alanen, Martti, Nurmi, Kim, Pettersson   +8 more
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Quantitative Real-Time PCR in aDNA Research

2011
Quantitative real-time PCR (qPCR) is a technique that is widely used in the field of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing.
Bunce, Michael, Oskam, C., Allentoft, M.
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Quantitative Real-Time RT-PCR

2011
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA, NARDON, ERMANNO, PRACELLA, DANAE, BONIN, Serena   +3 more
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Validation of a quantitative method for real time PCR kinetics

Biochemical and Biophysical Research Communications, 2002
Real time RT-PCR is the most sensitive method for quantitation of gene expression levels. The accuracy can be dependent on the mathematical model on which the quantitative methods are based. The generally accepted mathematical model assumes that amplification efficiencies are equal at the exponential phase of the reactions for the same amplicon ...
Liu, W., Saint, D.
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Detection of pathogenic leptospires by real-time quantitative PCR

Journal of Medical Microbiology, 2005
Definitive diagnosis of leptospirosis has traditionally depended upon the isolation of leptospires from clinical specimens or the demonstration of seroconversion in paired acute and convalescent serum samples. Both of these approaches require expertise not routinely available in clinical laboratories and usually result in delayed diagnosis ...
Paul N, Levett, Roger E, Morey, Renee L, Galloway, Danielle E, Turner, Arnold G, Steigerwalt, Leonard W, Mayer   +5 more
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New FRET primers for quantitative real-time PCR

Analytical and Bioanalytical Chemistry, 2007
FRET primer real-time PCR chemistry depends on internally labeled primers with FRET dyes linked to their 3' end. The best distance between the FRET dyes for obtaining the largest signal and the lowest background is six nucleotides. In this study the forward primer was labeled with FAM and the reverse primer with Texas red; the labeled primers meet in ...
Ashraf I, Ahmad, Jahan B, Ghasemi
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Real-Time Quantitative PCR for the Detection of Fetal Aneuploidies

2008
In prenatal analysis, one of the major concerns is the detection of fetal aneuploidies. Several molecular methods have been described recently for the rapid analysis of amniotic fluid and chorionic villi. Fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) of short tandem repeats are already implemented in prenatal ...
Bernhard G, Zimmermann, Lech, Dudarewicz
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Diagnosis of aerobic vaginitis by quantitative real-time PCR

Archives of Gynecology and Obstetrics, 2016
To evaluate a real-time PCR-based technique to quantify bacteria associated with aerobic vaginitis (AV) as a potential test.Vaginal samples from 100 women were tested by wet-mount microscopy, gram stain and quantitative real-time PCR targeting Enterobacteriacea, Staphylococcus spp., Streptococcus spp., Enterococcus spp., Escherichia coli, Streptococcus
Rumyantseva, T. A., Bellen, G., Savochkina, Y. A., Guschin, A. E., Donders, G.G.G.   +4 more
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Real-Time Quantitative PCR, Pathogen Detection and MIQE

2012
Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination
Gemma, Johnson, Tania, Nolan, Stephen A, Bustin   +2 more
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