Differential quantification of CYP2D6 gene copy number by four different quantitative real-time PCR assays [PDF]
, 2010 Copy number variations (CNVs) in the CYP2D6 gene contribute to interindividual variation in drug metabolism. As the most common duplicated allele in Asian populations is the nonfunctional CYP2D6*36 allele, the goal of this study was to identify CNV ...
Flockhart, David A. +5 more
core +1 more source
HBV DNA Quantitation Using Real-time PCR
Annals of Laboratory Medicine, 2006 Accurate measurement of the concentration of hepatitis B virus (HBV) DNA in clinical samples is important for the appropriate treatment of patients and evaluation of their therapeutic responses. In addition, the concentration of HBV DNA in the serum of patients with chronic HBV infection has a very broad range.
Jeong, Heo +7 more
openaire +2 more sources
A multiplex quantitative real time PCR to detect Xanthomonas axonopodis pv.allii from onion seeds : [P2-26] [PDF]
, 2010 Bacterial blight of onion is a seed-borne emerging disease threatening world onion production, and causing damage to other Allium crops. Its causal agent, #Xanthomonas axonopodis pv.
Escalon, Aline +3 more
core
Quantitative real-time PCR for the clinical detection of Helicobacter pylori
Genetics and Molecular Biology, 2007 Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human ...
Marcelo Lima Ribeiro +4 more
doaj +1 more source
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia () Growth Hormone Receptor Type I [PDF]
, 2009 Quantization of gene expression requires that an accurate measurement of a specific transcript is made. In this paper, a quantitative reverse transcription-polymerase chain reaction (RT-PCR) by competition for tilapia growth hormone receptor type I is ...
Rodrí +5 more
core +1 more source
Investigating the role of DNA methyltransferases in the mantled somaclonal variation of oil palm [PDF]
, 2007 DNA methyltransferase (DMTases) genes have emerged as targets of interest in the exploration of epigenetic mechanisms underlying the mantled variant phenotype in oil palm.
Beulé, Thierry +3 more
core
First detection of Waddlia chondrophila in Africa using SYBR Green real-time PCR on veterinary samples. [PDF]
, 2013 Waddlia chondrophila is a strict intracellular microorganism belonging to the order Chlamydiales that has been isolated twice from aborted bovine fetuses, once in USA and once in Germany. This bacterium is now considered as an abortigenic agent in cattle.
Barkallah, M. +5 more
core +1 more source
Making standards for quantitative real-time pneumococcal PCR [PDF]
Biomolecular Detection and Quantification, 2014 Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1-1.2).
Morpeth, Susan C. +3 more
openaire +2 more sources
The International Journal of Biological Markers, 2006 The assessment of ERα, PgR and HER2 status is routinely performed today to determine the endocrine responsiveness of breast cancer samples. Such determination is usually accomplished by means of immunohistochemistry and in case of HER2 amplification by ...
M. Labuhn +8 more
doaj +1 more source
Quantitative Determination of Lentiviral Vector Particle Numbers by Real-Time PCR
BioTechniques, 2001 Here, we describe a quantitative, DNAbased, real-time PCR approach to determine the number of lentivirus particles that are present in vector preparations.
M. Scherr +4 more
doaj +1 more source