Clinical and Experimental Gastroenterology, 2020 Nabi Jomehzadeh,1– 3 Hazhir Javaherizadeh,4 Mansour Amin,2,3 Mohammad Rashno,1 Ali Teimoori5 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Infectious and Tropical Diseases Research Center ...
Jomehzadeh N +4 more
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PLoS ONE, 2017 Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. It is a unique approach to measure the absolute copy number of nucleic acid targets without the need of ...
Yogita Maheshwari +3 more
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RTPrimerDB : the portal for real-time PCR primers and probes [PDF]
, 2009 RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked to genome information from reference databases and ...
S. Lefever +7 more
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Validation of reference genes for gene expression studies in tartary buckwheat (Fagopyrum tataricum Gaertn.) using quantitative real-time PCR [PDF]
PeerJ, 2019 Quantitative real-time reverse transcriptase polymerase chain reaction is a sensitive technique for quantifying gene expression levels. By implementing three distinct algorithms (geNorm, normFinder and BestKeeper), we have validated the stability of the ...
Chenglei Li +10 more
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PCR-based assays for detecting Xanthomonas axonopodis pv. allii in onion seed [PDF]
, 2012 Bacterial blight of onion is an emerging disease threatening world onion production, and causing damage to other Allium crops. The causal agent, Xanthomonas axonopodis pv.
Chabirand, Aude +4 more
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Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms [PDF]
, 2006 Background Real-time PCR is the technique of choice for nucleic acid quantification. In the field of detection of genetically modified organisms (GMOs) quantification of biotech products may be required to fulfil legislative requirements.
Žel Jana +4 more
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A rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water [PDF]
, 2015 Background: Water and High Purity Water (HPW) distribution systems can be contaminated with human pathogenic microorganisms. This biocontamination may pose a risk to human health as HPW is commonly used in the industrial, pharmaceutical and clinical ...
Barry, Thomas +4 more
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Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process [PDF]
, 2008 12 pages, 2 figures, 4 tables.-- PMID: 19102748 [PubMed].-- PMCID: PMC2629474.[Background] The elucidation of gene expression patterns leads to a better understanding of biological processes.
Marino Expósito-Rodríguez +3 more
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Barrier protection via Toll-like receptor 2 signaling in porcine intestinal epithelial cells damaged by deoxynivalnol [PDF]
, 2016 Additional file 2. IPEC-J2 cells pretreated with TLR2 ligand maintained the expression of MCP-1, GM-CSF and TLR2 against DON exposure. IPEC-J2 cells pretreated with or without TLR2 ligand for 24 h were exposed to DON.
Byung-Chul Park +11 more
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Polymerase chain displacement reaction
BioTechniques, 2013 Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material.
Claire L. Harris +4 more
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