Results 111 to 120 of about 814,412 (288)

Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae [PDF]

, 2016
Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods.
Abdul Latif, Muhammad, Abdullah, Siti Nor Akmar, Ashkani, Sadegh, Azizi, Parisa, Mahmood, Maziah, Musa, Mohamed Hanafi, Sahebi, Mahbod, Yusop, Mohd Rafii   +7 more
core   +2 more sources

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Establishment of real-time rt-pcr assay for detection of mrna mycobacterium tuberculosis

Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2011
Current laboratory methods for monitoring the response to therapy for tuberculosis (TB) rely on mycobacterial culture. Their clinical usefulness is therefore limited by the slow growth rate of Mycobacterium tuberculosis.
Hồ Thị Thanh Thủy, Phạm Thảo Nguyên, Nguyễn Phan Thành, Huỳnh Xuân Linh, Lê Huyền Ái Thúy   +4 more
doaj  

Effects of volatile organic compound ether on cell responses and gene expressions in Arabidopsis [PDF]

Additional file 1: Table S1.
Kuo-Chih Lin, Yun-Ting Tseng
core   +2 more sources

Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs [PDF]

, 2008
In this work four species-specific primers and probes were designed and evaluated for the detection and quantification of bovine, ovine, swine and chicken mitochondrial DNA in feeds.
Ajmone_Marsan, P, Chegdani, F, Chiappini, B, Faggionato, E, Fontana, C, Frezza, D, Giambra, V, Lanni, L, Losio, N, Maccabiani, G, Saccares, S, Vaccari, G, von Holst, C   +12 more
core   +1 more source

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

Congenital Chagas disease in a Bolivian newborn in Bergamo (Italy) [PDF]

, 2018
Chagas disease (CD) is an uncommon disease in Europe. Its epidemiology has changed because of mass migration from Latin America to Europe. Herein we describe a congenital case of CD in a Bolivian newborn in Bergamo, the main city of residence for the ...
Bargiggia, Graziano, D'Antiga, Lorenzo, Farina, Claudio, Gabrielli, Simona, Ortalli, Gaia, Rodari, Paola, Ruggeri, Maurizio   +6 more
core  

Congenital cytomegalovirus, parvovirus and enterovirus infection in Mozambican newborns at birth: A cross-sectional survey [PDF]

, 2018
BACKGROUND: Congenital cytomegalovirus (cCMV) infection is the most prevalent congenital infection acquired worldwide, with higher incidence in developing countries and among HIV-exposed children.
Bassat Orellana, Quique, Calderon, Enrique J., Carrilho, Carla, Esteva, Cristina, Friaza, Vicente, Ismail, Mamudo Rafik, Lozano Dominguez, María del Carmen, Maculuve, Sónia Amós, Madrid, Lola, Menéndez, Clara, Muñoz-Almagro, Carmen, Nhampossa, Tacilta, Varo, Rosauro, Vieites, Begoña   +13 more
core   +3 more sources

Real-Time RT-PCR Detection of Equine Coronavirus

, 2016
Equine coronavirus (ECoV) is a recently identified equine virus, involved mainly in enteric infections. Since the ECoV discovery in 1999, only two real-time RT-PCRs have been developed for viral identification. In this chapter we describe a one-step real-time RT-PCR that has been routinely used in our laboratory for ECoV detection from fecal and ...
Miszczak, Fabien, Kin, Nathalie, Tesson, Vincent, Vabret, Astrid   +3 more
openaire   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source