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[Real-time polymerase chain reaction (RT-PCR)].
Annales Academiae Medicae Stetinensis, 2008The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology,
Katarzyna, Wiedro +2 more
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One-step real time RT-PCR for detection of microRNAs
Talanta, 2013Rapid and simple methods for microRNA (miRNA) detection are essential for biological research of miRNAs and clinical diagnosis. Here we describe a sensitive and specific real time RT-PCR (also RT-qPCR) method for miRNA quantification. The whole detection process including reverse transcription and PCR is performed in one PCR tube by a one-step ...
Jingli, Yan +4 more
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Real-time quantitative RT–PCR after laser-assisted cell picking
Nature Medicine, 1998The present study describes a technique for quantitation of mRNA in a few isotypic cells obtained from an intact organ structure by combining laser-assisted cell picking and real-time PCR. The microscopically controlled lasering of selected cells in stained tissue sections was applied to lung alveolar macrophages, which are unique in that they can ...
L, Fink +7 more
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Quantifying Aotus monkey cytokines by real-time quantitative RT-PCR
Cytokine, 2004Aotus spp. monkeys are considered the ideal model for studying the progress of malarial infection and the immune response it elicits. We describe the use of a recently developed technique, real-time quantitative RT-PCR, to quantify several Aotus monkey cytokine mRNAs involved in Th1/Th2 responses (IL-4, IL-10, TNF-beta and IFN-gamma).
Yago, Pico de Coaña +5 more
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Quantitation of ovine cytokine mRNA by real-time RT–PCR
Journal of Immunological Methods, 2006In this study we describe for the first time the dynamics of the expression of the cytokines, IL-1beta, IL-12p40, TNFalpha in ovine dendritic cells and macrophages after LPS stimulation. Real time RT-PCR was used for the quantitation of these cytokines and IL-4 and IFNgamma as well as two potential housekeeping genes (HKG), ATPase and GAPDH, in mRNAs ...
Sangeeta, Budhia +3 more
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Real-Time RT-PCR for CTG Repeat-Containing Genes
2004Myotonic dystrophy (DM1) is a neuromuscular disorder caused by a CTGn expansion in the 3'-untranslated region (UTR) of myotonic dystrophy protein kinase (DMPK). SIX5 is a homeodomain gene located just downstream of the repeat, and myotonic dystrophy WD protein (DMWD) is located close upstream of DMPK.
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REAL-TIME (RT-PCR) DETECTION OF MONKEYPOX VİRUS
2022Polimeraz Zincir Reaksiyonu (PCR), küçük DNA fragmanlarında belirli dizilerin hızlandırılmış amplifikasyonuna izin veren in vitro replikasyonudur. Hassas bir teknik olan PCR’nin, analiz edilecek kadar kopya üretmesi için yalnızca DNA izlerine ihtiyaç vardır.
TAHİROĞLU, Veysel +2 more
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โรคพิษสุนัขบ้าพบในสัตว์เลี้ยงลูกด้วยนม เป็นโรคที่ทำให้ผู้ป่วยเสียชีวิตด้วยอาการสมองอักเสบอย่างฉับพลัน สุนัขเป็นตัวการสำคัญในการแพร่เชื้อประจำท้องถิ่น การตรวจหาแอนติเจนในสมองสุนัขด้วยวิธี Direct fluorescent antibody (DFA) technique เป็นวิธีตรวจวินิจฉัยขั้นต้น อย่างไรก็ตาม การตรวจวินิจฉัยโดยวิธี TaqMan real-time one step RT-PCR เป็นวิธีทางอณูชีววิทยาท ...
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