Results 271 to 280 of about 814,412 (288)

Identification of nasal blood by real-time RT-PCR

Legal Medicine, 2012
A new approach for the identification of body fluid stains by comparing specific mRNA expression levels has been extensively studied in recent years. Here, we examine whether nasal blood, which is regarded as one of the most difficult types of blood to identify, can be identified by comparing mRNA expression levels of target genes specific to saliva ...
Koichi, Sakurada, Tomoko, Akutsu, Ken, Watanabe, Mineo, Yoshino   +3 more
openaire   +2 more sources

Real-time RT-PCR for norovirus screening in shellfish

Journal of Virological Methods, 2005
Real-time RT-PCR, combining amplification and detection of virus-specific amplicons, is a promising tool for norovirus detection in environmental or food samples such as shellfish. We developed a real-time RT-PCR assay based on one-step detection using single primer sets and probes for norovirus genogroups I and II.
Loisy, F, Atmar, RL, Guillon, P, Le Cann, P, Pommepuy, M, Le Guyader, FS   +5 more
openaire   +3 more sources

Monitoring Gene Expression: Quantitative Real-Time RT-PCR

2013
Two-step quantitative real-time RT-PCR (RT-qPCR), also known as real-time RT-PCR, kinetic RT-PCR, or quantitative fluorescent RT-PCR, has become the method of choice for gene expression analysis during the last few years. It is a fast and convenient PCR method that combines traditional RT-PCR with the phenomenon of fluorescence resonance energy ...
openaire   +2 more sources

Quantification of GPCR mRNA Using Real-Time RT-PCR

2011
Characterisation of G-protein-coupled receptor (GPCR) mRNA expression under normal, different pharmacological and pathological conditions in experimental animal models and human tissue biopsies by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) is a valuable approach to understand the regulation of GPCR expression.
Trond, Brattelid, Finn Olav, Levy
openaire   +2 more sources

[Real-time polymerase chain reaction (RT-PCR)].

Annales Academiae Medicae Stetinensis, 2008
The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology,
Katarzyna, Wiedro, Ewa, Stachowska, Dariusz, Chlubek   +2 more
openaire   +1 more source

One-step real time RT-PCR for detection of microRNAs

Talanta, 2013
Rapid and simple methods for microRNA (miRNA) detection are essential for biological research of miRNAs and clinical diagnosis. Here we describe a sensitive and specific real time RT-PCR (also RT-qPCR) method for miRNA quantification. The whole detection process including reverse transcription and PCR is performed in one PCR tube by a one-step ...
Jingli, Yan, Nan, Zhang, Cui, Qi, Xiangjun, Liu, Dihua, Shangguan   +4 more
openaire   +2 more sources

Real-time quantitative RT–PCR after laser-assisted cell picking

Nature Medicine, 1998
The present study describes a technique for quantitation of mRNA in a few isotypic cells obtained from an intact organ structure by combining laser-assisted cell picking and real-time PCR. The microscopically controlled lasering of selected cells in stained tissue sections was applied to lung alveolar macrophages, which are unique in that they can ...
L, Fink, W, Seeger, L, Ermert, J, Hänze, U, Stahl, F, Grimminger, W, Kummer, R M, Bohle   +7 more
openaire   +2 more sources

Quantifying Aotus monkey cytokines by real-time quantitative RT-PCR

Cytokine, 2004
Aotus spp. monkeys are considered the ideal model for studying the progress of malarial infection and the immune response it elicits. We describe the use of a recently developed technique, real-time quantitative RT-PCR, to quantify several Aotus monkey cytokine mRNAs involved in Th1/Th2 responses (IL-4, IL-10, TNF-beta and IFN-gamma).
Yago, Pico de Coaña, Carlos, Barrero, Isabela, Cajiao, Catalina, Mosquera, Manuel Elkin, Patarroyo, Manuel Alfonso, Patarroyo   +5 more
openaire   +2 more sources

Quantitation of ovine cytokine mRNA by real-time RT–PCR

Journal of Immunological Methods, 2006
In this study we describe for the first time the dynamics of the expression of the cytokines, IL-1beta, IL-12p40, TNFalpha in ovine dendritic cells and macrophages after LPS stimulation. Real time RT-PCR was used for the quantitation of these cytokines and IL-4 and IFNgamma as well as two potential housekeeping genes (HKG), ATPase and GAPDH, in mRNAs ...
Sangeeta, Budhia, Lisa F, Haring, Ian, McConnell, Barbara A, Blacklaws   +3 more
openaire   +2 more sources

Real-Time RT-PCR for CTG Repeat-Containing Genes

2004
Myotonic dystrophy (DM1) is a neuromuscular disorder caused by a CTGn expansion in the 3'-untranslated region (UTR) of myotonic dystrophy protein kinase (DMPK). SIX5 is a homeodomain gene located just downstream of the repeat, and myotonic dystrophy WD protein (DMWD) is located close upstream of DMPK.
openaire   +2 more sources