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Comparative transcriptomic of Stevia rebaudiana provides insight into rebaudioside D and rebaudioside M biosynthesis

Plant Physiology and Biochemistry, 2021
Rebaudioside D (Reb D) and rebaudioside M (Reb M) are commercially important low/no-calorie natural sweeteners. However, they are present in a minor proportion of all steviol glycosides (SGs) in Stevia rebaudiana Bertoni (S. rebaudiana). Strain-dependent deviation in Reb D and Reb M biosynthesis is one key breach for breeding of S.
Heng Yin, Xiaochen Jia, Heng Yin
exaly   +3 more sources

Enhancement of Rebaudioside M Production by Structure-Guided Engineering of Glycosyltransferase UGT76G1

Journal of Agricultural and Food Chemistry, 2022
Owing to zero-calorie and advanced organoleptic properties similar to sucrose, the plant-derived rebaudioside M (Reb M) has been considered as a next generation sweetener. However, a low content of Reb M in Stevia rebaudiana Bertoni and low enzymatic activity of UGT76G1, which is an uridine diphosphate glucose (UDPG)-dependent glycosyltransferase with ...
Baodang Guo   +6 more
openaire   +2 more sources

In vitro metabolism of rebaudioside B, D, and M under anaerobic conditions: Comparison with rebaudioside A

Regulatory Toxicology and Pharmacology, 2014
The hydrolysis of the steviol glycosides rebaudioside A, B, D, and M, as well as of steviolbioside (a metabolic intermediate) to steviol was evaluated in vitro using human fecal homogenates from healthy donors under anaerobic conditions. Incubation of each of the rebaudiosides resulted in rapid hydrolysis to steviol. Metabolism was complete within 24h,
Sidd, Purkayastha   +5 more
openaire   +2 more sources

Convergent synthesis of steviol glycosides rebaudioside a and M

Carbohydrate Research
Steviol glycosides, a natural health-focused sweetener, is receiving increasing attention. Herein, an efficient and concise approach for the convergent synthesis of Rebaudioside A and M has been devised. This method features a AuCl3-tBuCN cooperative catalysis to achieve highly 1, 2-trans stereoselective glycosidation of a C2-branched trisaccharide ...
Yuanyuan Li   +5 more
openaire   +2 more sources

Enhancing Rebaudioside M Synthesis via Introducing Sulfur-Mediated Interactions between Glycosyltransferase UGT76G1 and Rebaudioside D

Journal of Agricultural and Food Chemistry
Rebaudioside M (Reb M), a zero-calorie sweetener with high sweetness, faces production challenges due to its low yield and purity. UGT76G1, a uridine diphosphate glucose (UDPG)-dependent glycosyltransferase, forms a β-1,3-glycosidic bond with rebaudioside D (Reb D) to produce Reb M but with an efficiency lower than that for stevioside (ST).
Fangwei Song   +7 more
openaire   +2 more sources

Multi‐Enzyme Cascade Reaction of Crude Enzyme Strategy for the Economical and Efficient Bioconversion of Rebaudioside A to Rebaudioside M

Biotechnology Journal
ABSTRACT Rebaudioside M (Reb M) is a natural sweetener with high sweetness, low caloric content, and an excellent sensory profile. However, it's extremely low natural abundance in Stevia rebaudiana leaves renders conventional extraction processes costly, inefficient, and ...
Yuanyuan Huang   +3 more
openaire   +1 more source

A review on rebaudioside M: The next generation steviol glycoside and noncaloric sweetener

Journal of Food Science
Abstract So far, the use of artificial low‐calorie sweeteners, like sucralose, saccharin, and so on, to replace the conventional‐based sugars has not succeeded due to the long‐term adverse health effects, for example, hypertension, and not well‐known safety stand.
Clinton E. Okonkwo   +8 more
openaire   +2 more sources

Fusion glycosyltransferase design for enhanced conversion of rebaudioside A into rebaudioside M in cascade

Molecular Catalysis, 2023
Xiaoqiang Wang   +4 more
openaire   +1 more source

A sustainable strategy for biosynthesis of Rebaudioside M using bifunctional enzyme

Biochemical Engineering Journal
Natural low-calorie rebaudioside M (Reb M), one of the most ideal sweeteners, was traditionally extracted from plants, which is time- and energy-consuming. The bioconversion of low-value rebaudioside A (Reb A) is green and economical. A series of hybrid enzymes of UGT76G1 and EUGT11 were designed and constructed to highly efficiently convert Reb A to ...
Baoqi Wang, Yuanyuan Ma, Hao Song
openaire   +1 more source

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