Results 11 to 20 of about 54,407 (151)

Targeting homologous recombination repair to potentiate fluoroquinolone efficacy in Mycobacterium abscessus [PDF]

mLife
mLife, EarlyView.
Shuai Wang, Md Shah Alam, Buhari Yusuf, Jingran Zhang, Ziwen Lu, Xiaofan Zhang, H. M. Adnan Hameed, Mst. Sumaia Khatun, Chunyu Li, Lijie Li, Aleksey A. Vatlin, Aweke M. Belachew, Xirong Tian, Yamin Gao, Cuiting Fang, Rogers P. Zhang, Jun Li, Xinyue Wang, Liqiang Feng, Li Wan, Tianyu Zhang   +20 more
wiley   +2 more sources

A Lambda Red Recombinase‐Assisted CRISPR/Cas9 System for Efficient and Precise Genome Editing in Agrobacterium tumefaciens [PDF]

Plant Biotechnology Journal, EarlyView.
ABSTRACT Agrobacterium tumefaciens is a powerful delivery tool for plant genetic transformation and a pivotal platform for agricultural biotechnology. However, laboratory strains of Agrobacterium have remained genetically unoptimized for decades, with genome engineering hindered by inefficient, endogenous homologous recombination systems.
Minmin Zheng, Rui Liu, Wei‐Chang Huang, Zuren Li, Kai Hua   +4 more
wiley   +2 more sources

Bacillus subtilis RarA Acts as a Positive RecA Accessory Protein [PDF]

Frontiers in Microbiology, 2020
Ubiquitous RarA AAA+ ATPases play crucial roles in the cellular response to blocked replication forks in pro- and eukaryotes. Here, we provide evidence that absence of RarA reduced the viability of ΔrecA, ΔrecO, and recF15 cells during unperturbed growth.
Hector Romero, Hector Romero, Hector Romero, Ester Serrano, Rogelio Hernández-Tamayo, Rogelio Hernández-Tamayo, Begoña Carrasco, Paula P. Cárdenas, Silvia Ayora, Peter L. Graumann, Peter L. Graumann, Juan C. Alonso   +11 more
doaj   +2 more sources

Biochemical characterization of Borrelia burgdorferi's RecA protein. [PDF]

PLoS ONE, 2017
RecA plays key roles in DNA recombination, replication and repair. Mutation of recA in the Lyme disease spirochete, Borrelia burgdorferi, fails to produce some of the phenotypes expected from study of recA mutation in other organisms.
Shu Hui Huang, Madison A Hart, Matthew Wade, McKayla R Cozart, Siobhan L McGrath, Kerri Kobryn   +5 more
doaj   +2 more sources

Radiation‐Triggered Chemokine‐Secreting Probiotics for Boron Neutron Capture Therapy Based Radioimmunotherapy [PDF]

Advanced Science, EarlyView.
Engineered Escherichia coli Nissle 1917 with a tannic acid‐mediated BPA coating enables tumor‐targeted boron delivery for BNCT. The low‐level incidental radiation generated during BNCT activate a RecA‐driven circuit to induce CXCL10, while radiation‐enhanced bacterial colonization triggers in situ vaccination and systemic antitumor immunity.
Yang Liu, Yuzhong Qian, Shaobo Huang, Bingbing Li, Yue Yu, Xiang Cheng, Chang Chen, Jiheng Wang, Maosong Yang, Lizheng Liang, Xiancai Meng, Zhigang Liu   +11 more
wiley   +2 more sources

Mechanistic insights into the structure and function of the RecA C-terminal tail [PDF]

Communications Biology
Bacterial RecA protein oligomerizes on single-stranded DNA (ssDNA) in the presence of ATP to form an active filament, RecA*, which is essential for homologous recombination and the activation of bacterial DNA damage response (SOS response).
Lu Su, Xiaofan Li, Feifan Wang, Mengzhen Wang, Yimin Lu, Yuyuan Zheng, Anmin Gao, Lu Chen, Yajun Guo, Mengchuan Xu, Yu Feng, Ning Dong, Chun Zhou   +12 more
doaj   +2 more sources

Characterizing RecA-Independent Induction of Shiga toxin2-encoding Phages by EDTA Treatment [PDF]

, 2012
The bacteriophage life cycle has an important role in Shiga toxin (Stx) expression. The induction of Shiga toxin-encoding phages (Stx phages) increases toxin production as a result of replication of the phage genome, and phage lysis of the host cell also
Muniesa Maite, Lejla Imamovic (180270), Lejla Imamovic, Maite Muniesa (66350), Maite Muniesa, Imamovic Lejla, Muniesa Pérez, Ma. Teresa, Muniesa Pérez, Ma Teresa, Imamovic, Lejla   +8 more
core   +3 more sources

Growth phase variation in cell and nucleoid morphology in a Bacillus subtilis recA mutant [PDF]

, 2001
The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects.
Piggot, P J, Sciochetti, S A, Blakely, G W, Stephen A. Sciochetti, Patrick J. Piggot, Garry W. Blakely   +5 more
core   +1 more source

A RecA protein surface required for activation of DNA polymerase V. [PDF]

PLoS Genetics, 2015
DNA polymerase V (pol V) of Escherichia coli is a translesion DNA polymerase responsible for most of the mutagenesis observed during the SOS response.
Angela J Gruber, Aysen L Erdem, Grzegorz Sabat, Kiyonobu Karata, Malgorzata M Jaszczur, Dan D Vo, Tayla M Olsen, Roger Woodgate, Myron F Goodman, Michael M Cox   +9 more
doaj   +1 more source

Two time constants for the binding of proteins to DNA from micromechanical data [PDF]

, 2000
Recent experimental advances allow the direct measurement of the force/extension behavior for DNA in the presence of strongly binding proteins. Such experiments reveal information about the cooperative mechanism of protein binding.
Turner, Matthew S., Matthew S. Turner
core   +1 more source