Results 21 to 30 of about 50,500 (200)

RNA-DNA hybridization promoted by E.coli RecA portein [PDF]

, 1992
RecA protein of E. coli plays a central regulatory role that is induced by damage to DNA and results in the inactivation of LexA repressor. In vitro, RecA protein binds preferentially to single-stranded DNA to form a nucleoprotein filament that can ...
Kirkpatrick, Dwight P., Radding, Charles M., Rao, B. Jagdeeshwar   +2 more
core   +1 more source

Directed Evolution of RecA Variants with Enhanced Capacity for Conjugational Recombination. [PDF]

PLoS Genetics, 2015
The recombination activity of Escherichia coli (E. coli) RecA protein reflects an evolutionary balance between the positive and potentially deleterious effects of recombination. We have perturbed that balance, generating RecA variants exhibiting improved
Taejin Kim, Sindhu Chitteni-Pattu, Benjamin L Cox, Elizabeth A Wood, Steven J Sandler, Michael M Cox   +5 more
doaj   +1 more source

Trichoderma strains isolated from Ilex paraguariensis ST. HIL: promising biocontrol agents with chitinolytic activity and plant growth promoter on Lycopersicum esculentum

Arab Journal of Basic and Applied Sciences, 2020
Ilex paraguariensis Saint Hilaire (St. Hil) is an important crop in the north of Argentina, mainly cultivated in the Misiones province. Trichoderma genera are well known for their biological control and plant growth-promoting properties.
Ana Clara López, Adriana Elizabet Alvarenga, Manuela Lizz Vereschuk, Ramona Celeste Barua, Pedro Darío Zapata, María Flavia Luna, Laura Lidia Villaba   +6 more
doaj   +1 more source

ATPase Activity of Bacillus subtilis RecA Affects the Dynamic Formation of RecA Filaments at DNA Double Strand Breaks

mSphere, 2022
RecA plays a central role in DNA repair and is a main actor involved in homologous recombination (HR). In vivo, RecA forms filamentous structures termed “threads,” which are essential for HR, but whose nature is still ill defined.
Rogelio Hernández-Tamayo, Niklas Steube, Thomas Heimerl, Georg K. A. Hochberg, Peter L. Graumann   +4 more
doaj   +1 more source

DNA Metabolism in Balance: Rapid Loss of a RecA-Based Hyperrec Phenotype. [PDF]

PLoS ONE, 2016
The RecA recombinase of Escherichia coli has not evolved to optimally promote DNA pairing and strand exchange, the key processes of recombinational DNA repair.
Irina V Bakhlanova, Alexandra V Dudkina, Elizabeth A Wood, Vladislav A Lanzov, Michael M Cox, Dmitry M Baitin   +5 more
doaj   +1 more source

Dynamics and Regulation of RecA Polymerization and De-Polymerization on Double-Stranded DNA. [PDF]

PLoS ONE, 2013
The RecA filament formed on double-stranded (ds) DNA is proposed to be a functional state analogous to that generated during the process of DNA strand exchange.
Hongxia Fu, Shimin Le, Kalappa Muniyappa, Jie Yan   +3 more
doaj   +1 more source

Dissecting the RecA-(In)dependent Response to Mitomycin C in Mycobacterium tuberculosis Using Transcriptional Profiling and Proteomics Analyses

Cells, 2021
Mycobacteria exploit at least two independent global systems in response to DNA damage: the LexA/RecA-dependent SOS response and the PafBC-regulated pathway.
Anna Brzostek, Przemysław Płociński, Alina Minias, Aneta Ciszewska, Filip Gąsior, Jakub Pawełczyk, Bożena Dziadek, Marcin Słomka, Jarosław Dziadek   +8 more
doaj   +1 more source

Single Molecule Study of the Polymerization of RecA on dsDNA: The Dynamics of Individual Domains

Frontiers in Molecular Biosciences, 2021
In the Escherichia coli, RecA plays a central role in the recombination and repair of the DNA. For homologous recombination, RecA binds to ssDNA forming a nucleoprotein filament.
Nitzan Maman, Nitzan Maman, Pramod Kumar, Amarjeet Yadav, Amarjeet Yadav, Mario Feingold, Mario Feingold   +6 more
doaj   +1 more source

Growth phase variation in cell and nucleoid morphology in a Bacillus subtilis recA mutant

, 2001
The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects.
Piggot, P J, Sciochetti, S A, Blakely, G W, Stephen A. Sciochetti, Patrick J. Piggot, Garry W. Blakely   +5 more
core   +1 more source

Spatial and temporal organization of RecA in the Escherichia coli DNA-damage response

eLife, 2019
The RecA protein orchestrates the cellular response to DNA damage via its multiple roles in the bacterial SOS response. Lack of tools that provide unambiguous access to the various RecA states within the cell have prevented understanding of the spatial ...
Harshad Ghodke, Bishnu P Paudel, Jacob S Lewis, Slobodan Jergic, Kamya Gopal, Zachary J Romero, Elizabeth A Wood, Roger Woodgate, Michael M Cox, Antoine M van Oijen   +9 more
doaj   +1 more source