Results 41 to 50 of about 810,282 (282)

Characterization of an intracellular humanized single-chain antibody to matrix protein (M1) of H5N1 virus.

PLoS ONE, 2022
We developed a human intracellular antibody based on the M1 protein from avian influenza virus H5N1 (A/meerkat/Shanghai/SH-1/2012) and then characterized the properties of this antibody.
He Sun, Guangmou Wu, Jiyuan Zhang, Yu Wang, Yue Qiu, Hongyang Man, Guoli Zhang, Zehong Li, Yuhuan Yue, Yuan Tian   +9 more
doaj   +1 more source

Shelf-Life Extension of Fc-Fused Single Chain Fragment Variable Antibodies by Lyophilization

Frontiers in Cellular and Infection Microbiology, 2021
Generation of sequence defined antibodies from universal libraries by phage display has been established over the past three decades as a robust method to cope with the increasing market demand in therapy, diagnostics and research.
Kai-Thomas Schneider, Toni Kirmann, Esther Veronika Wenzel, Esther Veronika Wenzel, Jan-Hendrik Grosch, Jan-Hendrik Grosch, Saskia Polten, Doris Meier, Marlies Becker, Paul Matejtschuk, Michael Hust, Giulio Russo, Giulio Russo, Stefan Dübel   +13 more
doaj   +1 more source

Characterization and epitope mapping of human monoclonal antibodies to PDC-E2, the immunodominant autoantigen of primary biliary cirrhosis [PDF]

, 1992
Further to define the epitopes of PDC-E2, the major autoantigen in primary biliary cirrhosis (PBC), we have developed and characterized five human monoclonal antibodies. These antibodies were derived by fusing a regional hepatic lymph node from a patient
Bassendine, Baum, Casali, Chambers, Coppel, Coppel, Cram, Flannery, Fregeau, Fregeau, Fujikura, Fusey, Fussey, Gershwin, Gershwin, Griffin, Griffin, James, Kaplan, Kunicki, Larrick, Leung, Leung, Mackay, Mackay, Mathews, McNeilage, Okamoto, Pollack, Ramsden, Reeves, Reimer, Robbins, Rokeach, Rowley, Ruther, Salemuddin, Sturgess, Sudin, Surh, Surh, Surh, Surh, Tan, Uibo, Van de Water, Van de Water, Van de Water, Van de Water, Williams   +49 more
core   +1 more source

Investigating Alternative Container Formats for Lyophilization of Biological Materials Using Diphtheria Antitoxin Monoclonal Antibody as a Model Molecule

Pharmaceutics, 2021
When preparing biological reference materials, the stability of the lyophilized product is critical for long-term storage, particularly in order to meet WHO International Standards, which are not assigned expiry dates but are expected to be in use for ...
Kiran P. Malik, Chinwe Duru, Paul Stickings, Esther Veronika Wenzel, Michael Hust, Paul Matejtschuk   +5 more
doaj   +1 more source

Advances in the design and development of oncolytic measles viruses. [PDF]

, 2015
A successful oncolytic virus is one that selectively propagates and destroys cancerous tissue without causing excessive damage to the normal surrounding tissue. Oncolytic measles virus (MV) is one such virus that exhibits this characteristic and thus has
Hutzen, Brian, Raffel, Corey, Studebaker, Adam W   +2 more
core   +1 more source

Unraveling the impact of SARS-CoV-2 mutations on immunity: insights from innate immune recognition to antibody and T cell responses

Frontiers in Immunology
Throughout the COVID-19 pandemic, the emergence of new viral variants has challenged public health efforts, often evading antibody responses generated by infections and vaccinations. This immune escape has led to waves of breakthrough infections, raising
Rafael Bayarri-Olmos, Rafael Bayarri-Olmos, Adrian Sutta, Adrian Sutta, Anne Rosbjerg, Anne Rosbjerg, Mie Mandal Mortensen, Charlotte Helgstrand, Per Franklin Nielsen, Laura Pérez-Alós, Beatriz González-García, Laust Bruun Johnsen, Finn Matthiesen, Thomas Egebjerg, Cecilie Bo Hansen, Alessandro Sette, Alessandro Sette, Alba Grifoni, Ricardo da Silva Antunes, Peter Garred, Peter Garred   +20 more
doaj   +1 more source

Characterisation of an Anti-Vaccinia Virus F13 Single Chain Fragment Variable from a Human Anti-Vaccinia Virus-Specific Recombinant Immunoglobulin Library

Viruses, 2022
Vaccinia virus (VACV) belongs to the genus Orthopoxvirus of the family Poxviridae. There are four different forms of infectious virus particles: intracellular mature virus (IMV), intracellular en-veloped virus (IEV), cell-associated enveloped virus (CEV)
Henrike P. Ahsendorf, Ulrike S. Diesterbeck, Sven-Kevin Hotop, Michael Winkler, Mark Brönstrup, Claus-Peter Czerny   +5 more
doaj   +1 more source

Gamma interferon-dependent clearance of cytomegalovirus infection in salivary glands [PDF]

, 1992
Cytomegalovirus (CMV), similar to other members of the Herpesviridae family, can establish both persistent and latent infections. Each of the CMVs that are found in many animal species replicates in the salivary gland, and oral secretion represents a ...
Jonjic, Stipan, Koszinowski, Ulrich H., Lucin, Pero, Pavic, Ivica, Polic, Bojan   +4 more
core   +2 more sources

NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies

mAbs, 2020
The mechanism of action of recombinant IgG2/4 antibodies involves blocking of their target without the induction of effector functions. Examples are eculizumab (Soliris®), which is used clinically to block complement factor C5, as well as anti-human CD14
Corinna Lau, Martin Berner McAdam, Grethe Bergseth, Algirdas Grevys, Jack Ansgar Bruun, Judith Krey Ludviksen, Hilde Fure, Terje Espevik, Anders Moen, Jan Terje Andersen, Tom Eirik Mollnes   +10 more
doaj   +1 more source

The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis [PDF]

, 2017
The frequency of sandfly-host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (SGH); however, it is necessary ...
Alten, Anderson, Bongiorno, Cortes, Cortes, Courtenay, Di Muccio, Drahota, Gradoni, Kostalova, Maia, Maresca, Maroli, Martín-Martín, Oliva, Prates, Ramalho-Ortigão, Rohousova, Rossi, Vlkova, Vlkova, Volf, Volf, Volf   +23 more
core   +3 more sources