Results 141 to 150 of about 12,163 (157)

[Study on recombinant BCG].

Kekkaku : [Tuberculosis], 1991
For the purpose to establish the system to express foreign antigen from Mycobacterium bovis BCG. We have cloned, sequenced and expressed genes for secreting proteins, alpha antigen, MPB64, MPB57 and MPB70 from M. bovis BCG. The upstreams and structural genes were characterized. The gene for alpha antigen of Mycobacterium kansasii was also characterized.
K, Matsuo, R, Yamaguchi, A, Yamazaki, K, Terasaka, S, Nagai, H, Tasaka, C, Abe, M, Totsuka, H, Yukitake, K, Kobayashi   +9 more
openaire   +1 more source

Secretion of human interleukin 2 by recombinant Mycobacterium bovis BCG

Infection and Immunity, 1995
The human interleukin 2 (huIL-2) gene was introduced into Mycobacterium bovis BCG by using the integrative vector pMV306. To express and secrete huIL-2 from BCG, two different plasmids, CI and CII, were made. In CI, the huIL-2-encoding region was under the control of the alpha-antigen promoter of BCG; in CII, the expression of huIL-2 was regulated by ...
D, Kong, D Y, Kunimoto
openaire   +2 more sources

Gene replacement by homologous recombination in Mycobacterium bovis BCG

Molecular Microbiology, 1995
SummaryGene replacement by homologous recombination is a powerful tool for fundamental studies of gene function, as well as allowing specific attenuation of pathogens, but has proved difficult to achieve for Mycobacterium tuberculosis. We have used a plasmid‐based test system to demonstrate the occurrence of homologous recombination in the tuberculosis
E, Norman, O A, Dellagostin, J, McFadden, J W, Dale   +3 more
openaire   +2 more sources

Immunotherapy of Experimental Bladder Cancer with Recombinant BCG Expressing Interferon-γ

Journal of Immunotherapy, 2004
One of the most potent immunotherapies presently used is the application of Bacillus Calmette Guérin (BCG) to prevent recurrences of superficial bladder cancer. Despite its successful use, nonresponders and certain side effects remain a major obstacle.
Judith, Arnold, Elizabeth C, de Boer, Michael A, O'Donnell, Andreas, Böhle, Sven, Brandau   +4 more
openaire   +2 more sources

Recombinant Mycobacterium bovis BCG for immunotherapy in nonmuscle invasive bladder cancer

Applied Microbiology and Biotechnology, 2015
In the past three decades, intravesical instillation of Mycobacterium bovis bacille Calmette-Guérin (BCG) has been used for treating bladder cancer and it still remains at the forefront of immunotherapy for cancer patients. Although BCG-based therapy is the most effective intravesical therapy for this kind of tumor and represents the only agent known ...
K R, Begnini, J H, Buss, T, Collares, F K, Seixas   +3 more
openaire   +2 more sources

Immunogenicity of recombinant BCG producing the GRA1 antigen from Toxoplasma gondii

Vaccine, 1999
Toxoplasmosis is a major parasitic disease, responsible for foetopathy in humans and domestic animals, especially sheep. Toxoplasma gondii infection generally protects immunocompetent hosts against subsequent reinfection, suggesting that efficacious vaccines can be developed against this disease. Excreted/secreted T.
P, Supply, P, Sutton, S N, Coughlan, K, Bilo, E, Saman, A J, Trees, M F, Cesbron Delauw, C, Locht   +7 more
openaire   +2 more sources

Efficacy and safety of live recombinant BCG vaccines.

Developments in biological standardization, 1995
BCG has a long history of safe use in humans and is one of the best adjuvants known. The use of newer production methods may further reduce the risk of adverse side-effects. Early results with experimental animals have shown BCG to be an effective live recombinant delivery vehicle for several foreign vaccine antigens.
M S, Hanson, G P, Bansal, S, Langermann, C K, Stover, I, Orme   +4 more
openaire   +1 more source

Recombinant BCG vaccines

Vaccine, 2001
N, Ohara, T, Yamada
openaire   +2 more sources

Recombinant BCG as an antigen delivery system.

Cellular and molecular biology (Noisy-le-Grand, France), 1994
BCG, an attenuated vaccine strain of Mycobacterium bovis, has been engineered for use as an antigen delivery vehicle. Various genetic and molecular biological techniques were recently developed for use in the manipulation of mycobacteria. Vectors for the expression of foreign antigens in BCG have been constructed and used to express genes from bacteria,
openaire   +1 more source

Development and Analysis of Recombinant BCG Vector Systems

AIDS Research and Human Retroviruses, 1992
T R, Fuerst, V F, de la Cruz, G P, Bansal, C K, Stover   +3 more
openaire   +2 more sources