Results 1 to 10 of about 399,275 (342)

A standardized set of pNX vectors for enhanced soluble expression of recombinant proteins in E. coli using small fusion tags [PDF]

Microbial Cell Factories
Background The production of recombinant proteins in Escherichia coli (E. coli) is often hampered by the formation of inclusion bodies. While fusion tags can enhance solubility, existing systems are hampered by a lack of standardization, with tags ...
Li-Zhen Luo, Wen-Bin Zhang, Zhe Hu, Ling-Hua Zhang, Jin-Cheng Ma, Xue-Bin Jiang   +5 more
doaj   +2 more sources

Production of recombinant SARS-COV-2 proteins and diphtheria toxoid CRM197-based fusion [PDF]

The Ukrainian Biochemical Journal, 2021
The quickly emerged global COVID-19 pandemic raised a desperate need in the development of protecting vaccines targeting this disease. Therefore, a generation of effective producers of recombinant SARS-CoV-2 proteins became an urgent task.
O. I. Krynina, S. I. Romaniuk, O. B. Gorbatiuk, O. H. Korchynskyi, А. V. Rebriiev, Ya. S. Kulyk, Ye. O. Kozadaieva, A. A. Siromolot, M. M. Guzyk, D. V. Kolybo, S. V. Komisarenko   +10 more
doaj   +1 more source

Fusion with heat-resistant obscure (Hero) proteins have the potential to improve the molecular property of recombinant proteins

PLoS ONE, 2022
Although recombinant proteins are widely used in biotechnology and pharmaceutical industries, improving their solubility and stability is often a challenging issue.
Eri Morimoto, Kotaro Tsuboyama, Yukihide Tomari   +2 more
doaj   +2 more sources

The B1 Domain of Streptococcal Protein G Serves as a Multi-Functional Tag for Recombinant Protein Production in Plants

Frontiers in Plant Science, 2022
Plants have long been considered a cost-effective platform for recombinant production. A recently recognized additional advantage includes the low risk of contamination of human pathogens, such as viruses and bacterial endotoxins. Indeed, a great advance
Shi-Jian Song, Hai-Ping Diao, Byeongho Moon, Areum Yun, Inhwan Hwang   +4 more
doaj   +1 more source

Pharmacokinetics of recombinant bifunctional fusion proteins [PDF]

Expert Opinion on Drug Metabolism & Toxicology, 2012
The development of biotechnology has enabled the creation of various recombinant fusion proteins as a new class of biotherapeutics. The uniqueness of fusion proteins lies in their ability to fuse two or more protein domains, providing vast opportunities to generate novel combinations of functions.
Xiaoying, Chen, Jennica L, Zaro, Wei-Chiang, Shen   +2 more
openaire   +2 more sources

Characterization of a novel type of HIV-1 particle assembly inhibitor using a quantitative Luciferase-Vpr packaging-based assay [PDF]

, 2011
The HIV-1 auxiliary protein Vpr and Vpr-fusion proteins can be copackaged with Gag precursor (Pr55Gag) into virions or membrane-enveloped virus-like particles (VLP).
Elisabeth Errazuriz, Eugene Oliver Major, Florence Souquet, Gaëlle Gonzalez, Pascale Coric, Pierre Boulanger, Sandrina DaFonseca, Saw See Hong, Serge Bouaziz, Serge Turcaud   +9 more
core   +18 more sources

Study of native SMAC protein production in the pUbiq expression system: Molecular cloning, biosynthesis and molecular modelling

Electronic Journal of Biotechnology, 2022
Background: In the process of recombinant protein biosynthesis affinity tags are efficient tools to achieve the expected purity and yield during the purification steps.
Pál Salamon, Csongor K. Orbán, Katalin Molnár-Nagy, Zita Kovács, Klára Váncsa, Emese Bálint, Ildikó Miklóssy, Beáta Albert, Gyöngyi Tar, Szabolcs Lányi   +9 more
doaj   +1 more source

Refinement of the Fusion Tag PagP for Effective Formation of Inclusion Bodies in Escherichia coli

Microbiology Spectrum, 2023
Methods for efficient insoluble protein production require further exploration. PagP, an Escherichia coli outer membrane protein with high β-sheet content, could function as an efficient fusion partner for inclusion body-targeted expression of ...
Xuefeng Li, Baorong Zhang, Quan Hu, Changchao Chen, Jiahua Huang, Lu Liu, Shengbin Wang   +6 more
doaj   +1 more source

Osteoinductive recombinant silk fusion proteins for bone regeneration [PDF]

Acta Biomaterialia, 2017
Protein polymers provide a unique opportunity for tunable designs of material systems due to the genetic basis of sequence control. To address the challenge of biomineralization interfaces with protein based materials, we genetically engineered spider silks to design organic-inorganic hybrid systems.
Dinjaski, N, Plowright, R, Zhou, S, Belton, DJ, Perry, CC, Kaplan, DL   +5 more
openaire   +3 more sources

DEVELOPMENT OF THE VACCINE BASED ON THE RECOMBINANT ANTIGENS OF PSEUDOMONAS AERUGINOSA

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Aim. The aim is obtaining, investigation and selection of recombinant antigens for inclusion theirs into the against Pseudomonas vaccine. Materials and methods. The genes encoding of the outer  membrane proteins F, L and I and Exotoxin A were synthesized
N. A. Mihailova, E. M. Zimina, A. V. Soldatenkova, A. A. Kaloshin   +3 more
doaj   +1 more source