Results 1 to 10 of about 6,420,091 (287)

Recombinant proteins in plants [PDF]

BMC Proceedings, 2014
Background Over the last few decades, several studies have shown that plants can be a viable option for producing functional recombinant proteins with a wide range of structural characteristics [1]. In addition, the potential benefit for developing countries is a prominent feature that we have recently addressed [2]. Plant-produced recombinant proteins
Marcelo M. Brigido, Simpson Andrew, O'Keefe Barry, André M. Murad, Giovanni R. Vianna, Ana Claudia Guerra Araujo, Waters Michael, Elíbio L. Rech, Nicolau B. da Cunha, Cristiano Lacorte, Aparecida Maria Fontes, Caballero Otavia   +11 more
openaire   +3 more sources

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Frontiers in Bioengineering and Biotechnology, 2019
Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind.
Nagesh K. Tripathi, Ambuj Shrivastava
doaj   +2 more sources

Overview of the Purification of Recombinant Proteins [PDF]

Current Protocols in Protein Science, 2015
AbstractWhen the first version of this unit was written in 1995, protein purification of recombinant proteins was based on a variety of standard chromatographic methods and approaches, many of which were described and mentioned throughoutCurrent Protocols in Protein Science. In the interim, there has been a shift toward an almost universal usage of the
P. Wingfield
openaire   +4 more sources

Producing recombinant proteins in Vibrio natriegens [PDF]

Microbial Cell Factories
The diversity of chemical and structural attributes of proteins makes it inherently difficult to produce a wide range of proteins in a single recombinant protein production system.
Matthew Smith, José Sánchez Hernández, Simon Messing, Nitya Ramakrishnan, Brianna Higgins, Jennifer Mehalko, Shelley Perkins, Vanessa E. Wall, Carissa Grose, Peter H. Frank, Julia Cregger, Phuong Vi Le, Adam Johnson, Mukul Sherekar, Morgan Pagonis, Matt Drew, Min Hong, Stephanie R. T. Widmeyer, John-Paul Denson, Kelly Snead, Ivy Poon, Timothy Waybright, Allison Champagne, Dominic Esposito, Jane Jones, Troy Taylor, William Gillette   +26 more
doaj   +2 more sources

Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins [PDF]

Plants, 2022
Recombinant proteins are the most important product of current industrial biotechnology. They are indispensable in medicine (for diagnostics and treatment), food and chemical industries, and research.
Sergey M. Rozov, Alla A. Zagorskaya, Yuri M. Konstantinov, Elena V. Deineko   +3 more
doaj   +2 more sources

Stabilizing additives added during cell lysis aid in the solubilization of recombinant proteins. [PDF]

PLoS ONE, 2012
Insoluble recombinant proteins are a major issue for both structural genomics and enzymology research. Greater than 30% of recombinant proteins expressed in Escherichia coli (E. coli) appear to be insoluble.
David J Leibly, Trang Nhu Nguyen, Louis T Kao, Stephen N Hewitt, Lynn K Barrett, Wesley C Van Voorhis   +5 more
doaj   +2 more sources

Synthesized Anti-HER2 Trastuzumab-MCC-DM1 Conjugate: An Evaluation of Efficacy and Cytotoxicity [PDF]

Iranian Journal of Immunology, 2023
Background: Trastuzumab is a humanized monoclonal antibody that targets site-specifically human epidermal growth factor-2 receptor (HER2) cell surface antigen overexpressed in approximately 20% of human breast carcinomas. Despite its positive therapeutic
Soodabeh Shafiee, Roya Mirzaei, Malihe Salehi, Neda Jalili, Amir Taheri, Leila Farahmand   +5 more
doaj   +1 more source

Engineering and production of recombinant bovine pancreatic ribonuclease enzyme (RNase A) as a potential therapeutic [PDF]

مجله بیوتکنولوژی کشاورزی, 2021
Objective  Immunotoxins are one of the most promising ways in therapeutic fields, specially cancer therapy which have a unique toxin-antibody structure, and kill the cancer cell by passing through the cell membrane and entering the target cell.
Masoume Vakili-Azghandi, Mohammadreza Nassiri, Shahrokh Ghovvati, Ali Javadmanesh   +3 more
doaj   +1 more source

HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors

Frontiers in Bioengineering and Biotechnology, 2021
Animal cell-based expression platforms enable the production of complex biomolecules such as recombinant proteins and viral vectors. Although most biotherapeutics are produced in animal cell lines, production in human cell lines is expanding.
Evan Tan, Cara Sze-Hui Chin, Zhi Lim, S. Ng   +3 more
semanticscholar   +1 more source

Recombinant Protein Stability in Cyanobacteria [PDF]

ACS Synthetic Biology, 2021
The living cell possesses extraordinary molecular and biochemical mechanisms by which to recognize and efficiently remove foreign, damaged, or denatured proteins. This essential function has been a barrier to the overexpression of recombinant proteins in most expression systems. A notable exception is the overexpression in E.
Xianan Zhang, Nico Betterle, Diego Hidalgo Martinez, Anastasios Melis   +3 more
openaire   +5 more sources