Expression of recombinant 35 kDa fragment of VP2 protein of canine parvovirus using Escherichia coli expression system [PDF]
Background and Aim: Canine parvovirus (CPV) is one of the most common viral infections in dogs, causing acute hemorrhagic gastroenteritis and high mortality. Vaccination effectively prevents CPV infection.
Natnaree Inthong+8 more
doaj +1 more source
Extraction from the herbs was performed using the Soxhlet method. Various formula was synthesized for niosomes containing the extracts through thin film synthesis technique, and the most efficient formulation was selected.
Akhlaghi Milad+8 more
doaj +1 more source
HIV-1 Gag Specific IgG Response in Mice Immunized with Vp22-Gag Vaccine Candidate [PDF]
Background : Stimulation of Gag-specific CD8+ T-cell response, associated with reduction in viremia, viral replication control, and slow disease progression. Effective CD8+ T cell response is also influenced by CD4+ T cells.
Bekti, H. S. (Heri)
core +1 more source
Recombinant plasma proteins [PDF]
For almost 50 years, the fractionation of human plasma has been the sole possible source of a wide range of therapeutic proteins–such as coagulation factors, anticoagulants, immunoglobulins, and albumin – essential to the treatment of serious congenital or acquired bleeding or immunological diseases. In the last 20 years, the application of recombinant
openaire +3 more sources
The recent advancements in the early detection of cancer biomarkers by DNAzyme-assisted aptasensors
Clinical diagnostics rely heavily on the detection and quantification of cancer biomarkers. The rapid detection of cancer-specific biomarkers is of great importance in the early diagnosis of cancers and plays a crucial role in the subsequent treatments ...
Hossein Kamali+5 more
doaj +1 more source
Ebola virus VP35 induces high-level production of recombinant TPL-2–ABIN-2–NF-κB1 p105 complex in co-transfected HEK-293 cells [PDF]
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells.
Apostolaki+47 more
core +1 more source
Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories
As a consequence of Covid-19 pandemic, the basic lab consumables are in shortage, especially in the low-income countries. Thus, the main objective of the present study is to develop and evaluate homemade solution to isolate plasmid.
Mohamed A. Elnagar+6 more
doaj +1 more source
Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells [PDF]
Specific antibodies against rap1A and rap1B small GTP-binding proteins were generated by immunization of rabbits with peptides derived from the C-terminus of the processed proteins.
Frank, R.+5 more
core +1 more source
Triple negative breast cancer (TNBC) is the most aggressive and lethal subtype of breast cancer. It is associated with a very poor prognosis and intrinsically resistant to several conventional and targeted chemotherapy agents and has a 5-year survival ...
Behrad Darvishi+2 more
doaj +1 more source
Identification of Breast Cancer Associated Putative Functional Single Nucleotide Polymorphisms in the Iranian Population through In Silico Analyses [PDF]
Previous studies have found several distinct alleles at both levels of transcriptional activity and protein-DNA binding manners in breast cancer patients vs. healthy individuals through multi-step experimental approaches.
Vida Nadafi Sichani+4 more
doaj +1 more source