Plant-Based Cell-Free Transcription and Translation of Recombinant Proteins [PDF]
113124Plant cell-free lysates contain all the cellular components of the protein biosynthesis machinery, providing an alternative to intact plant cells, tissues, and whole plants for the production of recombinant proteins. Cell-free lysates achieve rapid
Schillberg, Stefan +3 more
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Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression [PDF]
The agroinfiltration of plant tissue is a robust method that allows the rapid and transient expression of recombinant proteins. Using wild-type plants as biomass, agroinfiltration exploits the ability of plants to synthesize even complex multimeric ...
Schillberg, Stefan +3 more
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Improving Recombinant Protein Recovery from Plant Tissue Using Heat Precipitation [PDF]
147157Plants are increasingly viewed as suitable expression hosts for the production of recombinant proteins, especially when oxidative folding and/or posttranslational modification is essential for protein stability and functionality.
Spiegel, Holger
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Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor [PDF]
S.161-172Tobacco BY-2 cells are an attractive platform for the manufacture of a variety of biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up cultivation of human IgG-secreting BY-2 cells in a 200 L orbitally shaken ...
Rasche, S., Raven, N., Schillberg, S.
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Improvement of downstream processing of recombinant proteins by means of genetic engineering [PDF]
Flaschel E, Friehs K. Improvement of downstream processing of recombinant proteins by means of genetic engineering. Biotechnology Advances. 1993;11(1):31-77.The rapid advancement of genetic engineering has allowed to produce an impressive number of ...
Flaschel, Erwin, Friehs, Karl
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The renin-angiotensin-aldosterone system (RAAS) signaling pathways and cancer: foes versus allies
The renin-angiotensin-aldosterone system (RAAS), is an old system with new fundamental roles in cancer biology which influences cell growth, migration, death, and metastasis.
Bahareh Hassani +2 more
doaj +1 more source
Recombination of Fragmented Proteins [PDF]
Directed evolution is a powerful method for generating novel molecules with desirable properties. In developing a new sensor to screen for protein-protein interactions, Tafelmeyer et al. report a clever strategy to evolve heterodimeric "split proteins" from a monomer in this issue of Chemistry & Biology.
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Recombinant plasma proteins [PDF]
For almost 50 years, the fractionation of human plasma has been the sole possible source of a wide range of therapeutic proteins–such as coagulation factors, anticoagulants, immunoglobulins, and albumin – essential to the treatment of serious congenital or acquired bleeding or immunological diseases. In the last 20 years, the application of recombinant
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Roles of the AtErcc1 protein in recombination [PDF]
SummaryAtercc1, the recently characterized Arabidopsis homologue of the Ercc1 (Rad10) protein, is a key component of nucleotide excision repair as part of a structure‐specific endonuclease which cleaves 5′ to UV photoproducts in DNA. This endonuclease also acts in removing overhanging non‐homologous DNA ‘tails’ in synapsed recombination intermediates ...
Dubest, Sandra +2 more
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Prospective Use of Brown Spider Venom Toxins as Therapeutic and Biotechnological Inputs
Brown spider (genus Loxosceles) venoms are mainly composed of protein toxins used for predation and defense. Bites of these spiders most commonly produce a local dermonecrotic lesion with gravitational spread, edema and hemorrhage, which together are ...
Luiza Helena Gremski +10 more
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