Results 41 to 50 of about 2,510,027 (306)

Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories

AMB Express, 2022
As a consequence of Covid-19 pandemic, the basic lab consumables are in shortage, especially in the low-income countries. Thus, the main objective of the present study is to develop and evaluate homemade solution to isolate plasmid.
Mohamed A. Elnagar, Mohamed F. Ibrahim, Magdy Albert, Maya M.Talal, Mahmoud M. Abdelfattah, Ehab El-Dabaa, Reham Helwa   +6 more
doaj   +1 more source

Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells [PDF]

, 1992
Specific antibodies against rap1A and rap1B small GTP-binding proteins were generated by immunization of rabbits with peptides derived from the C-terminus of the processed proteins.
Schwaner, I., Frank, R., Gausepohl, H., Seifert, Roland, Schultz, Günter, Klinz, F. J.   +5 more
core   +1 more source

Porcine antibody profiles of 33 Mycoplasma hyopneumoniae fusion proteins from M. hyopneumoniae natural infection but not vaccination

Veterinary Medicine and Science, 2023
Background Mycoplasma hyopneumoniae, the primary pathogen responsible for porcine enzootic pneumonia, reduces average daily weight gain and causes substantial economic losses to the pig industry worldwide.
Yaru Ning, Yujiao Yang, Yaqin Tian, Yun Zhang, Wenyi Luo, Yukang Wen, Yaoqin Zhou, Honglei Ding   +7 more
doaj   +1 more source

Fusing the 3’UTR of seed storage protein genes leads to massive recombinant protein accumulation in seeds

Scientific Reports, 2023
The demand for recombinant proteins is rising dramatically, and effective production systems are currently being developed. The production of recombinant proteins in plants is a promising approach due to its low cost and low risk of contamination of the ...
Masatake Kanai, Masaya Sugiyama, Maki Kondo, Kenji Yamada, Mikio Nishimura, Shoji Mano   +5 more
doaj   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Optimisation of the production of cathepsin L1 from a recombinant saccharomyces cerevisiae [PDF]

, 2002
Cathepsin L1 is a cysteine protease that has been previously isolated and functionally expressed in Saccharomyces cerevisiae. It has the potential to be employed as a vaccine for liver-fluke disease in cattle and other ruminants.
O'Donovan, Eimear C
core   +1 more source

Targeted drug delivery using nanobodies to deliver effective molecules to breast cancer cells: the most attractive application of nanobodies

Cancer Cell International
Targeted drug delivery is one of the attractive ways in which cancer treatment can significantly reduce side effects. In the last two decades, the use of antibodies as a tool for accurate detection of cancer has been noted. On the other hand, the binding
Mohadeseh Haji Abdolvahab, Pegah Karimi, Nasrin Mohajeri, Mohammad Abedini, Hamed Zare   +4 more
doaj   +1 more source

Flexible approaches are required for successful production of recombinant proteins in plants

Medicine Science, 2022
Production of recombinant proteins has increased significantly due to the ever-increasing demand in research laboratories, medical fields, and agriculture.
Nilufer Gun, Tarlan Mamedov
doaj   +1 more source

Identification of Breast Cancer Associated Putative Functional Single Nucleotide Polymorphisms in the Iranian Population through In Silico Analyses [PDF]

Journal of Cell and Molecular Research, 2020
Previous studies have found several distinct alleles at both levels of transcriptional activity and protein-DNA binding manners in breast cancer patients vs. healthy individuals through multi-step experimental approaches.
Vida Nadafi Sichani, Seyed Alireza Emami, Morteza Bitaraf Sani, Mohammadreza Nassiri, Vinod Gopalan   +4 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source