Results 271 to 280 of about 797,107 (316)

Capillary electrophoresis of recombinant proteins

Journal of Chromatography B: Biomedical Sciences and Applications, 1997
Many naturally occurring proteins which are used therapeutically have been cloned and expressed in large quantities in bacterial, yeast or mammalian systems. Purification of these proteins by column chromatography generates high purity products with low levels of host protein contaminants.
K A, Denton, S A, Tate
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SCHEMA-Guided Protein Recombination

2004
Publisher Summary This chapter examines the different aspects of SCHEMA-guided protein recombination. SCHEMA is a scoring function that predicts which elements in homologous proteins can be swapped without disturbing the integrity of the structure. Using the structural coordinates of the parent proteins, SCHEMA identifies pairs of residues that are ...
Silberg, Jonathan J., Endelman, Jeffrey B., Arnold, Frances H.   +2 more
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Recombinant Proteins in Vaccine Development

2003
The outer membrane of Neisseria meningitidis contains a variety of proteins with the potential for inclusion in new meningococcal vaccines (1). Studies on the vaccine potential of these proteins would be facilitated by the production of pure recombinant protein, free from other components of the Neisseria outer membrane.
M, Christodoulides, K A, Jolley, J E, Heckels   +2 more
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Preparation of recombinant neuritin protein

Protein Expression and Purification
Neuritin plays an important role in promoting nerve injury repair and maintaining synaptic plasticity, making it a potential therapeutic target for the treatment of nerve injury and neurodegenerative diseases. The present study aimed to obtain an active, unlabeled neuritin protein.
Pingping, Meng, Liyan, Zhu, Jiatong, Guo, Yuanyuan, Li, Yu, Wei, Jiawei, Sun, Jingling, Zhu   +6 more
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Recombinant protein production and streptomycetes

Journal of Biotechnology, 2012
The biopharmaceutical market has come a long way since 1982, when the first biopharmaceutical product, recombinant human insulin, was launched. Just over 200 biopharma products have already gained approval. The global market for biopharmaceuticals which is currently valued at over US$99 billion has been growing at an impressive compound annual growth ...
Jozef, Anné, Bárbara, Maldonado, Jan, Van Impe, Lieve, Van Mellaert, Kristel, Bernaerts   +4 more
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Purification of BmR1 Recombinant Protein

The Protein Journal, 2010
This paper describes a refinement in the purification step that facilitated the downstream recovery of high purity BmR1 recombinant protein, which is a protein used as a test reagent in the commercialized rapid tests for detection of lymphac filariasis i.e. Brugia Rapid™ and panLF rapid™.
Norsyahida, Arifin, Madihah, Basuni, Chew Ai, Lan, Ahmad Ramli Mohd, Yahya, Rahmah, Noordin   +4 more
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The expression of recombinant proteins in yeasts

Current Opinion in Biotechnology, 1996
The methylotrophic yeasts Hansenula polymorpha and Pichia pastoris are rapidly becoming the systems of choice for the expression of recombinant proteins in yeast. However, the powerful genetic techniques available in Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe are still exploited to establish models to study medically ...
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Selenomethionine Labeling of Recombinant Proteins

2007
Selenomethionine incorporation is a standard method for determining the phases in protein crystallography by single- or multiwavelength anomalous dispersion. Recombinant expression of selenomethionine-containing protein in non-auxotrophic Pichia pastoris strains yield an incorporation of about 50%.
Anna M, Larsson, T Alwyn, Jones
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Recombinant Protein Expression in Nicotiana

2010
Recombinant protein pharmaceuticals are now widely used in treatment of chronic diseases, and several recombinant protein subunit vaccines are approved for human and veterinary use. With growing demand for complex protein pharmaceuticals, such as monoclonal antibodies, manufacturing capacity is becoming limited.
Nobuyuki, Matoba, Keith R, Davis, Kenneth E, Palmer   +2 more
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Challenges in production of recombinant proteins

Bioprocess and Biosystems Engineering, 2007
The sequencing of the human genome and genomes from lower species has led molecular biology research into the era of proteomics. Starting from the cDNA’s, a large number of proteins have to be expressed in quantities large enough to allow determining of their structure and function, which forms the basis for the development of new pharmaceutical ...
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