Current affinity approaches for purification of recombinant proteins
Cogent Biology, 2019 Recombinant proteins have wide applications in the development of pharmaceutical compounds, industrial applications of enzymes, and basic proteomics research.
S. Mahmoodi +4 more
semanticscholar +1 more source
The recent advancements in the early detection of cancer biomarkers by DNAzyme-assisted aptasensors
Journal of Nanobiotechnology, 2022 Clinical diagnostics rely heavily on the detection and quantification of cancer biomarkers. The rapid detection of cancer-specific biomarkers is of great importance in the early diagnosis of cancers and plays a crucial role in the subsequent treatments ...
Hossein Kamali +5 more
doaj +1 more source
Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories
AMB Express, 2022 As a consequence of Covid-19 pandemic, the basic lab consumables are in shortage, especially in the low-income countries. Thus, the main objective of the present study is to develop and evaluate homemade solution to isolate plasmid.
Mohamed A. Elnagar +6 more
doaj +1 more source
High‐yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii
Plant Biotechnology Journal, 2017 Summary Microalga‐based biomanufacturing of recombinant proteins is attracting growing attention due to its advantages in safety, metabolic diversity, scalability and sustainability.
E. Ramos-Martinez +2 more
semanticscholar +1 more source
Molecular Therapy: Nucleic Acids, 2017 Triple negative breast cancer (TNBC) is the most aggressive and lethal subtype of breast cancer. It is associated with a very poor prognosis and intrinsically resistant to several conventional and targeted chemotherapy agents and has a 5-year survival ...
Behrad Darvishi +2 more
doaj +1 more source
Kinetics of protein-DNA interaction: facilitated target location in sequence-dependent potential [PDF]
Biophys. J. 87:4021-4035 (2004), 2004 Recognition and binding of specific sites on DNA by proteins is central for many cellular functions such as transcription, replication, and recombination. In the process of recognition, a protein rapidly searches for its specific site on a long DNA molecule and then strongly binds this site.
arxiv +1 more source
Identification of Breast Cancer Associated Putative Functional Single Nucleotide Polymorphisms in the Iranian Population through In Silico Analyses [PDF]
Journal of Cell and Molecular Research, 2020 Previous studies have found several distinct alleles at both levels of transcriptional activity and protein-DNA binding manners in breast cancer patients vs. healthy individuals through multi-step experimental approaches.
Vida Nadafi Sichani +4 more
doaj +1 more source
The clinical spectrum of SMA‐PME and in vitro normalization of its cellular ceramide profile
Annals of Clinical and Translational Neurology, Volume 9, Issue 12, Page 1941-1952, December 2022., 2022 Abstract Objective The objectives of this study were to define the clinical and biochemical spectrum of spinal muscular atrophy with progressive myoclonic epilepsy (SMA‐PME) and to determine if aberrant cellular ceramide accumulation could be normalized by enzyme replacement.
Michelle M. Lee +16 more
wiley +1 more source
Effect of IL-2 co-expressed or co-inoculated with immuno-dominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice [PDF]
Iranian Journal of Basic Medical Sciences, 2019 Objective(s): The results of studies on vaccine development for foot-and-mouth disease (FMD) virus show that the use of inactivated vaccines for FMD virus is not completely effective.
Mohammad Doosti +4 more
doaj +1 more source
ExpressInHost: A codon tuning tool for the expression of recombinant proteins in host microorganisms [PDF]
arXiv, 2021 ExpressInHost (https://gitlab.com/a.raguin/expressinhost) is a GTK/C++ based user friendly graphical interface that allows tuning the codon sequence of an mRNA for recombinant protein expression in a host microorganism. Heterologous gene expression is widely implemented in biotechnology companies and academic research laboratories.
arxiv