Results 51 to 60 of about 6,420,190 (386)

An overview of heterologous expression host systems for the production of recombinant proteins

, 2016
| With the advent of recombinant DNA technology, cloning and expression of numerous mammalian genes in different systems have been explored to produce many therapeutics and vaccines for human and animals in the form of recombinant proteins. But selection
AmithA, ReenA, Gomes, sonnAhAllipuRA, munivenkAtAppA, ByReGowdA, B. Veeregowda, V. Balamurugan   +7 more
semanticscholar   +1 more source

Flexible approaches are required for successful production of recombinant proteins in plants

Medicine Science, 2022
Production of recombinant proteins has increased significantly due to the ever-increasing demand in research laboratories, medical fields, and agriculture.
Nilufer Gun, Tarlan Mamedov
doaj   +1 more source

Recombinant protein expression in biofilms

AIMS Microbiology, 2019
Biofilm research is usually focused on the prevention or control of biofilm formation. Recently, the significance of the biofilm mode of growth in biotechnological applications received increased attention. Since biofilm reactors show many advantages over suspended cell reactors, especially in their higher biomass density and operational stability ...
Luciana C. Gomes, Ana Azevedo, Alexandra Soares, Filipe Mergulhão   +3 more
openaire   +3 more sources

Thermostable exoshells fold and stabilize recombinant proteins

Nature Communications, 2017
The expression and stabilization of recombinant proteins is fundamental to basic and applied biology. Here we have engineered a thermostable protein nanoparticle (tES) to improve both expression and stabilization of recombinant proteins using this ...
S. Deshpande, Nihar Masurkar, Vallerinteavide Mavelli Girish, M. Desai, Goutam Chakraborty, J. M. Chan, C. Drum   +6 more
semanticscholar   +1 more source

Design and expression of TolC as a recombinant protein vaccine against Shigella flexneri and evaluation of immunogenic response in mice [PDF]

arXiv, 2023
Background: Shigella is one of the major cause of dysenteric diarrhea, which is known shigelosis. Shigelosis causes 160,000 deaths annually of diarrheal disease in a global scale especially children less than 5 years old. No licensed vaccine is available against shigelosis, therefore, efforts for develop an effective and safe vaccine against shigella ...
arxiv  

Designing Smart Materials with Recombinant Proteins.

Macromolecular Bioscience, 2017
Recombinant protein design allows modular protein domains with different functionalities and responsive behaviors to be easily combined. Inclusion of these protein domains can enable recombinant proteins to have complex responses to their environment (e ...
Sydney E Hollingshead, Charng-Yu Lin, Julie C. Liu   +2 more
semanticscholar   +1 more source

Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins

Microbial Cell Factories, 2015
BackgroundLipopolysaccharide (LPS), also referred to as endotoxin, is the major constituent of the outer leaflet of the outer membrane of virtually all Gram-negative bacteria. The lipid A moiety, which anchors the LPS molecule to the outer membrane, acts
U. Mamat, Kathleen Wilke, D. Bramhill, A. Schromm, B. Lindner, T. Kohl, J. Corchero, A. Villaverde, L. Schaffer, S. Head, C. Souvignier, Timothy C. Meredith, R. Woodard   +12 more
semanticscholar   +1 more source

Elucidation of interface interactions between a dehydratase domain and an acyl carrier protein in cremimycin polyketide synthase

FEBS Letters, EarlyView.
In modular polyketide synthases, the dehydratase (DH) domain catalyzes the dehydration reaction of the β‐hydroxyacyl unit attached to the cognate acyl carrier protein (ACP) domain. However, it is unclear how DH interacts with ACP during the reaction. In this study, we identified DH–ACP interface residues, providing the first detailed insights into DH ...
Kaede Kotagiri, Haruka Tachibana, Daisuke Kawasaki, Taichi Chisuga, Toma Kashima, Shinya Fushinobu, Fumitaka Kudo, Tadashi Eguchi, Akimasa Miyanaga   +8 more
wiley   +1 more source

Ablation of LRP6 in alpha‐smooth muscle actin‐expressing cells abrogates lung inflammation and fibrosis upon bleomycin‐induced lung injury

FEBS Letters, EarlyView.
Low‐density lipoprotein receptor‐related protein 6 (LRP6) is a key receptor for the Wnt antagonist Dickkopf1 (DKK1). DKK1 protein expression is induced in a bleomycin (BLM)‐induced lung injury model. We show that DKK1 induces proinflammatory and profibrotic genes in lung fibroblasts.
Eun‐Ah Sung, Mikhail G. Dozmorov, SuJeong Song, Theingi Aung, Min Hee Park, Patricia J. Sime, Wook‐Jin Chae   +6 more
wiley   +1 more source

Targeted drug delivery using nanobodies to deliver effective molecules to breast cancer cells: the most attractive application of nanobodies

Cancer Cell International
Targeted drug delivery is one of the attractive ways in which cancer treatment can significantly reduce side effects. In the last two decades, the use of antibodies as a tool for accurate detection of cancer has been noted. On the other hand, the binding
Mohadeseh Haji Abdolvahab, Pegah Karimi, Nasrin Mohajeri, Mohammad Abedini, Hamed Zare   +4 more
doaj   +1 more source