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Hosts and Heterologous Expression Strategies of Recombinant Toxins for Therapeutic Purposes [PDF]

Toxins, 2023
The production of therapeutic recombinant toxins requires careful host cell selection. Bacteria, yeast, and mammalian cells are common choices, but no universal solution exists.
Giuseppina Pitari, Rodolfo Ippoliti, Luana di Leandro   +2 more
exaly   +6 more sources

Recombinant Alpha, Beta, and Epsilon Toxins of Clostridium perfringens: Production Strategies and Applications as Veterinary Vaccines

Toxins, 2016
Clostridium perfringens is a spore-forming, commensal, ubiquitous bacterium that is present in the gastrointestinal tract of healthy humans and animals. This bacterium produces up to 18 toxins.
Ângela Moreira, Marcelo Mendonça, Carlos E P Cunha   +2 more
exaly   +4 more sources

Advanced Situation with Recombinant Toxins: Diversity, Production and Application Purposes [PDF]

International Journal of Molecular Sciences, 2023
Today, the production and use of various samples of recombinant protein/polypeptide toxins is known and is actively developing. This review presents state-of-the-art in research and development of such toxins and their mechanisms of action and useful ...
Ilya Lyagin, Elena Efremenko, Aysel Aslanli   +2 more
exaly   +3 more sources

Molecular engineering of Komagataella phaffii for venom toxin production [PDF]

Applied Microbiology and Biotechnology
Animal venoms constitute a rich source of bioactive peptides and proteins with high target specificity, representing valuable scaffolds for therapeutic development.
Francielle Almeida Cordeiro, Karla de Castro Figueiredo Bordon, Henrique Ranieri Covali-Pontes, Isabela Gobbo Ferreira, Gisele Adriano Wiezel, Isadora Lino Mendes, Malson Neilson de Lucena, Eliane Candiani Arantes   +7 more
doaj   +2 more sources

Immunogenicity of two representative American consensus scorpion neurotoxins from the genera Tityus and Centruroides. [PDF]

PLoS Neglected Tropical Diseases
Two consensus scorpion toxins derived from venoms of the genera Centruroides (NATx, North America), and Tityus (SATx, South America) were rationally designed and recombinantly expressed in Escherichia coli Origami. Both recombinant proteins were produced
Samuel Cardoso-Arenas, Miguel Angel Mejia-Sanchez, Ricardo Miranda-Blancas, Herlinda Clement, Lilu Corrales-García, Ivan Arenas, Gerardo Pavel Espino-Solís, Hildaura Acosta, Marcos H Salazar, Gerardo Corzo   +9 more
doaj   +2 more sources

Proteomic characterization of Tityus championi venom and recombinant expression of its major neurotoxin [PDF]

Journal of Venomous Animals and Toxins including Tropical Diseases
Background: Tityus championi is a species endemic to the southern Talamanca Mountain Range, along the border region between Costa Rica and Panama, and has been associated with severe clinical cases.
Milena del C. Muñoz-Agames, Marcos H. Salazar, Herlinda Clement, Lorena Hernández-Orihuela, Magdalena Hernández-Ortiz, Sergio Encarnación-Guevara, John Cleghorn, Hildaura Acosta, Gerardo Corzo   +8 more
doaj   +3 more sources

Purified zymogens reveal mechanisms of snake venom metalloproteinase auto-activation [PDF]

eLife
Snake venoms contain diverse mixtures of toxins that evolved to incapacitate prey, but in humans, they cause extensive pathology following snakebite envenomation. In viper venom, some of the most potent toxins are the haemorrhagic and coagulopathic snake
Sophie Hall, Iara Aime Cardoso, Mark C Wilkinson, Maria Molina Carretero, Srikanth Lingappa, Bronwyn Rand, Dakang Shen, Johara Boldrini-França, Richard Stenner, Stefanie Kate Menzies, Georgia Balchin, Konrad Kamil Hus, Renaud Vincentelli, Andrew Mumford, Alastair Poole, Nicholas R Casewell, Imre Berger, Christiane Schaffitzel   +17 more
doaj   +2 more sources

Evaluation and Comparison of Clostridium Epsilon-Alpha Fusion Gene Expression Using Different Commercial Expression Vector [PDF]

Archives of Razi Institute, 2021
Clostridium perfringens and Clostridium septicum are gram-positive, anaerobic, spore-forming rods and pathogens for humans and livestock, which are widespread in nature as well as human and animal digestive systems. C.
H. R Sepehrifar, R Pilehchian Langroudi, S Ataei, A Haddadi   +3 more
doaj   +1 more source

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

Frontiers in Bioengineering and Biotechnology, 2022
Animal venoms are complex mixtures containing peptides and proteins known as toxins, which are responsible for the deleterious effect of envenomations. Across the animal Kingdom, toxin diversity is enormous, and the ability to understand the biochemical ...
Esperanza Rivera-de-Torre, Charlotte Rimbault, Timothy P. Jenkins, Christoffer V. Sørensen, Anna Damsbo, Natalie J. Saez, Yoan Duhoo, Celeste Menuet Hackney, Lars Ellgaard, Andreas H. Laustsen   +9 more
doaj   +1 more source

Expression, Purification and Refolding of a Human Na_V1.7 Voltage Sensing Domain with Native-like Toxin Binding Properties

Toxins, 2021
The voltage-gated sodium channel NaV1.7 is an important target for drug development due to its role in pain perception. Recombinant expression of full-length channels and their use for biophysical characterization of interactions with potential drug ...
Ryan V. Schroder, Leah S. Cohen, Ping Wang, Joekeem D. Arizala, Sébastien F. Poget   +4 more
doaj   +1 more source