Results 41 to 50 of about 356,092 (265)

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

Recombinant Bombyx mori Nucleopolyhedrovirus Harboring Green Fluorescent Protein

BioTechniques, 1998
The gene encoding the green fluorescent protein (gfp) under the control of the highly expressed Autographa californica nucleopolyhedrovirus (AcMNPV)-polyhedrin promoter has been introduced into the polyhedron (polh) locus of Bombyx mori ...
Deepak Sehgal, Karumathil P. Gopinathan
doaj   +1 more source

Characterization of a recombinant pseudorabies virus expressing porcine parvovirus VP2 protein and porcine IL-6

Virology Journal, 2020
Background Porcine parvovirus (PPV) and pseudorabies virus (PRV) are the important etiological agents of swine infectious diseases, resulting in huge economic losses to the Chinese swine industry. Interleukin-6 (IL-6) has the roles to support host immune
Hui-Hua Zheng, Lin-Qing Wang, Peng-Fei Fu, Lan-Lan Zheng, Hong-Ying Chen, Fang Liu   +5 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Efficient cross-protection against serotype 4/8a fowl adenoviruses (FAdVs): recombinant FAdV-4 with FAdV-8a Fiber

Microbiology Spectrum, 2023
Recently, the infection of fowl adenoviruses (FAdVs) in chicken flocks has caused huge economic losses to the poultry industry in China. Although immunization with Fiber protein can provide autogenous protection of FAdVs, no commercial multivalent ...
Yixuan Lu, Yaqin Yuan, Huiru Jiang, Zhenqi Xu, Yiwen Guo, Xudong Cao, Tuofan Li, Zhimin Wan, Hongxia Shao, Aijian Qin, Quan Xie, Jianqiang Ye   +11 more
doaj   +1 more source

RNA virus replication, transcription and recombination [PDF]

RNA Biology, 2011
In October of 2009 a group of researchers with a common interest in RNA virus reproduction met to present and discuss their recent findings on molecular mechanisms related to viral genome replicati...
White, K. Andrew, Enjuanes, Luis, Berkhout, Ben   +2 more
openaire   +3 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Cytocidal Effect of Irradiation on Gastric Cancer Cells Infected with a Recombinant Mammalian Orthoreovirus Expressing a Membrane-Targeted KillerRed

Pharmaceuticals
The outcomes of unresectable gastric cancer (GC) are unfavorable even with chemotherapy; therefore, a new treatment modality is required. The combination of an oncolytic virus and photodynamic therapy can be one of the promising modalities to overcome ...
Yoshinori Shirasaka, Kentaro Yamada, Tsuyoshi Etoh, Kazuko Noguchi, Takumi Hasegawa, Katsuhiro Ogawa, Takeshi Kobayashi, Akira Nishizono, Masafumi Inomata   +8 more
doaj   +1 more source

Recombination and Mutation in a New HP-PRRSV Strain (SD2020) from China

Viruses, 2023
A new HP-PRRSV strain (SD2020) was isolated from pigs with suspected highly pathogenic porcine reproductive and respiratory syndrome disease in a pig farm in Shandong Province, China, and its genome was sequenced. This pig farm has been using the VR-2332
Yang Xia, Tianying Zhang, Dengmei Gong, Juan Qi, Shenghai Jiang, Hao Yang, Xianchang Zhu, Yu Gan, Yi Zhang, Yanyan Han, Yan Li, Jida Li   +11 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source