Results 151 to 160 of about 528,821 (173)

Development and Application of a Rapid Field Detection Technology for DENV-2 Based on the HUDSON Nucleic Acid Extraction-Free/RT-RAA/CRISPR-Cas12a System. [PDF]

Viruses
Tan C, Xing S, Xie X, Chen X, Liu X, Wang W, Liu L, Zhou X, Wu J, Li C.   +9 more
europepmc   +1 more source

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Development of two recombinase-aided amplification assays combined with lateral flow dipstick (RAA-LFD) and real-time fluorescence (RF-RAA) for the detection of Frog virus 3-like ranaviruses

Fish and Shellfish Immunology
Frog virus 3-like ranaviruses (FV3-like viruses), particularly FV3 (Frog virus 3), represent typical species within the genus Ranavirus, primarily infecting amphibians and reptiles, thereby posing serious threats to aquaculture and biodiversity conservation. We designed a pair of universal primers and a probe targeting the conserved region of the major
Lang Gui
exaly   +4 more sources

Real-Time Reverse Transcription Recombinase-Aided Amplification Assay for Rapid Amplification of the N Gene of SARS-CoV-2

International Journal of Molecular Sciences, 2022
COVID-19 was officially declared a global pandemic disease on 11 March 2020, with severe implications for healthcare systems, economic activity, and human life worldwide.
Zhendong Guo, Cheng Zhang, Juxiang Liu
exaly   +2 more sources

Development of Recombinase-Aided Amplification (RAA)-Exo-Probe and RAA-CRISPR/Cas12a Assays for Rapid Detection of Campylobacter jejuni in Food Samples

Journal of Agricultural and Food Chemistry, 2022
Campylobacter jejuni is the major cause of campylobacteriosis, one of the most common foodborne illnesses worldwide. Here, we report the development of RAA-exo-probe and RAA-CRIPSR/Cas12a assays for the detection of C. jejuni in food samples. The two assays were found to be highly specific to C.
Shuai Zhi, Jinling Shen, Xingang Li, Yuan Jiang, Junxin Xue, Taisong Fang, Jin Xu, Xuan Wang, Yuhao Cao, Danting Yang, Zhiyuan Yao, Daniel Yu   +11 more
openaire   +2 more sources

Development of a real-time recombinase-aided amplification (RT-RAA) molecular diagnosis assay for sensitive and rapid detection of Toxoplasma gondii

Veterinary Parasitology, 2021
Toxoplasma gondii, a protozoan intracellular parasite, is present in a wide range of hosts, including virtually all species of warm-blooded vertebrates. Toxoplasmosis spreads to humans through a variety of pathways, including contaminated food or water, and close contact with various types of domestic animals.
Zhao-Hua, Wang, Wei, Zhang, Xiu-Zhong, Zhang, Xin-Ran, Yao, Wei, Huang, Hong, Jia, Xiao-Lei, Liu, Shao-Hua, Hou, Xiao-Jia, Wang   +8 more
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Development of Recombinase Aided Amplification (RAA)-Exo-Probe Assay for the Rapid Detection of Shiga Toxin-Producing Escherichia coli

Journal of AOAC International, 2023
Abstract Background Shiga toxin-producing Escherichia coli (STEC) is a significant cause of foodborne illness causing various gastrointestinal diseases including hemolytic uremic syndrome (HUS), the most severe form, which can lead to kidney failure or even death.
Yuhao Cao, Taisong Fang, Jinling Shen, Guodong Zhang, Dehua Guo, Lina Zhao, Yuan Jiang, Shuai Zhi, Lin Zheng, Xiaofei Lv, Zhiyuan Yao, Daniel Yu   +11 more
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Application of portable real-time recombinase-aided amplification (rt-RAA) assay in the clinical diagnosis of ASFV and prospective DIVA diagnosis

Applied Microbiology and Biotechnology, 2021
African swine fever, a serious infectious disease, has been found in many countries around the world over the last nearly 100 years, and causes untold damage to the economy wherever it occurs. Diagnosis is currently performed by real-time PCR, which is highly sensitive but can only be carried out in a diagnostic laboratory environment with ...
Zhao-Hua, Wang, Pei, Li, Xiao, Lin, Hong, Jia, Yi-Tong, Jiang, Xiao-Jia, Wang, Shao-Hua, Hou   +6 more
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Internally controlled recombinase-aided amplification (IC-RAA) assays for the detection of human papillomavirus genotypes 16 and 18 using extracted DNA and samples treated with nucleic acid releasing agent

Archives of Virology, 2020
Cervical cancer is primarily caused by persistent infection with high-risk human papillomavirus (HPV), and 70% of cases are associated with HPV16 and 18 infections. The objective of this study was to establish rapid, simple, and sensitive internally controlled recombinase-aided amplification (IC-RAA) assays for the detection of HPV16 and 18. The assays
Jinrong Wang, Jianli Liu, Guowei Song, Zhi Cao, Jing Pan, Xinna Li, Yuan Gao, Juju Qi, Ziwei Chen, Guohao Fan, Xueding Bai, Ruiqing Zhang, Ruihuan Wang, Qingxia Duan, Lixin Li, Xinxin Shen, Xuejun Ma   +16 more
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Rapid molecular diagnostic method for Gardnerella vaginalis based on CRISPR-Cas12a and recombinase-aided amplification (RAA)

Clinica Chimica Acta
Imbalance of the vaginal microbiota, particularly the overgrowth of Gardnerella vaginalis, is the primary cause of bacterial vaginosis (BV), which poses a significant threat to women's reproductive health. Therefore, early and rapid diagnosis of BV is crucial.
Tong, Jiang, Chuang, Zhang, Daqing, Wang, Zijing, Guo, Yong, Guo, Hua, Liu, Zhuo, Wang   +6 more
openaire   +2 more sources

Dual-Gene Based Recombinase-Aided Amplification (RAA) - Lateral Flow Strip for the Sensitive On-Site Detection of Yersinia enterocolitica in Food Samples

Journal of AOAC International
Abstract Background Yersinia enterocolitica is one of the most common foodborne pathogens worldwide. Currently, most detection methods are primarily focused on pathogenic strains of Y. enterocolitica; while it has been frequently observed that traditionally believed “non-pathogenic” strains of Y ...
Tiantian Zhou, Jinlin Shen, Yu Feng, Norman F Neumann, Min Xu, Hangtian Ma, Yuhao Cao, Yuan Jiang, Yuqiu Zhang, Junxin Xue, Jian Li, Shuai Zhi   +11 more
openaire   +2 more sources