Results 121 to 130 of about 131,465 (263)

A multiplex recombinase polymerase amplification assay combined with CRISPR/Cas12a for the detection of respiratory syncytial virus and respiratory adenovirus

Journal of International Medical Research
Objective Respiratory syncytial virus (RSV) and respiratory adenovirus (ADV) are two common pathogens that cause acute respiratory tract infections in children. We aimed to develop a rapid method for detecting both pathogens simultaneously.
Hongdan Gao, Du Yao, C. Qiang, Huan Meng, Xiaorong Liu, Zhihao Xing, Dongli Ma   +6 more
semanticscholar   +1 more source

Guidelines for Light‐Start One‐Pot RPA‐CRISPR‐Cas12a Assays for Foodborne Pathogen Detection

Food Safety and Health, EarlyView.
Light‐start one‐pot RPA‐CRISPR‐Cas12a detection schematic diagram. (A) Linear crRNA activation light‐start one‐pot workflow. (B) Caged crRNA activation light‐start one‐pot workflow. ABSTRACT Rapid and accurate detection of nucleic acids of foodborne pathogens is of vital importance for controlling the spread of foodborne diseases and safeguarding ...
Fan Li, Ruiting Yao, Xinxin Jiang, Haorui Zhu, Guoliang Li   +4 more
wiley   +1 more source

Self-partitioning SlipChip for slip-induced droplet formation and human papillomavirus viral load quantification with digital LAMP [PDF]

, 2020
Human papillomavirus (HPV) is one of the most common sexually transmitted infections worldwide, and persistent HPV infection can cause warts and even cancer.
Han, Xu, Ismagilov, Rustem F., Lai, Dongmei, Lyu, Weiyuan, Qu, Haijun, Shen, Feng, Wang, Qian, Yu, Mengchao, Yu, Ziqing   +8 more
core  

Repetitive, Marker-Free, Site-Specific Integration as a Novel Tool for Multiple Chromosomal Integration of DNA [PDF]

, 2013
We present a tool for repetitive, marker-free, site-specific integration in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB). The novelty
Albert, Bolotin, Breüner, Breüner, Brønsted, Campo, Casadaban, Christian Solem, Christiansen, Dale, Defoor, Gasson, Geu-Flores, Glick, Groth, Hanniffy, Holo, Hugenholtz, Jan Martinussen, Jendresen, Jensen, Kelly, Kia Vest Petersen, Kleerebezem, Maguin, Martín, Mierau, Nørholm, Palmeros, Peter Ruhdal Jensen, Peterbauer, Sambrook, Sauer, Solem, Solem, Solem, Terzaghi   +36 more
core   +1 more source

CRISPR Enabled Precision Oncology: From Gene Editing to Tumor Microenvironment Remodeling

Med Research, EarlyView.
CRISPR technology has progressed from a prokaryotic immune system to a diverse suite of editing platforms, including Cas nucleases, base and prime editors, and RNA‐targeting enzymes. These advances enable precise genomic and epigenomic interventions, high‐throughput functional screening, and immune engineering.
Kailai Li, Peixin Huang, Yue Qian, Anqi Lin, Jingjun He, Junyi Shen, Li Chen, Kai Miao, Jian Zhang   +8 more
wiley   +1 more source

Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA [PDF]

, 2016
Bovine tuberculosis is a zoonotic infectious disease caused by Mycobacterium bovis 19 that affects cattle and can cause tuberculosis in a range of wildlife animals.
Albert H, Albert H, David ST, Katoch VM, Srivastava K   +4 more
core   +2 more sources

Development and application of a novel recombinase polymerase amplification-Pyrococcus furiosus argonaute system for rapid detection of goose parvovirus

Poultry Science
Rapid and accurate detection of goose parvovirus (GPV) is crucial for controlling outbreaks and mitigating their economic impact on the poultry industry.
Yaqun Liu, Lianghui Chen, Zhenxia Zhang, Rong Zhang, Jinyue Xu, Peikui Yang, Yanjie Sun, Yicun Chen, Chengsong Xie, Min Lin, Yuzhong Zheng   +10 more
semanticscholar   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Small, EarlyView.
The review highlights nature‐inspired nanocarriers for CRISPR delivery, emphasizing viral vectors, extracellular vesicles, liposomes, and lipid nanoparticles. It discusses their roles in improving specificity, minimizing immunogenicity, and overcoming barriers in genome editing. Recent advancements, challenges, and therapeutic applications are explored,
Elizabeth Maria Clarissa, Mamata Karmacharya, Hyunmin Choi, Sumit Kumar, Yoon‐Kyoung Cho   +4 more
wiley   +1 more source

Stress-resistant plants and their production [PDF]

, 2012
The present invention relates to plant genes involved in negative regulation of resistance to biotic and/or abiotic stress and uses thereof. More particularly, the present invention relates to plants comprising an inactivated MADS-box gene function, and ...
Gantet, Pascal, Guiderdoni, Emmanuel, Khong, Ngan Giang, Morel, Jean-Benoit   +3 more
core