Results 81 to 90 of about 131,465 (263)

Enhanced heterogeneity of rpoB in Mycobacterium tuberculosis found at low pH.

, 2009
OBJECTIVES: The aim of this study was to gain an insight into the molecular mechanisms of the evolution of rifampicin resistance in response to controlled changes in the environment.
Allnutt, Jon, Arnold, Catherine, Bacon, Joanna, Bose, Alpana, Gillespie, Stephen H, Hatch, Kim A, Jenkins, Claire, McHugh, Timothy D, O'Sullivan, Denise M   +8 more
core   +1 more source

TFIIIC Localizes Budding Yeast ETC Sites to the Nuclear Periphery [PDF]

, 2012
Peer reviewedPublisher ...
Botsios, Sotirios, Donaldson, Anne Dunlop, Donze, David, Hiraga, Shinichiro   +3 more
core   +2 more sources

Low-cost recombinase polymerase amplification (RPA) v1

, 2021
This protocol describes the expression of enzymes and creation of a master mix for recombinase polymerase amplification (RPA) assays.
openaire   +1 more source

Recombinase Polymerase Amplification (RPA) versus PCR for ancient DNA library amplification [PDF]

, 2019
Background : Recombinase Polymerase Amplification (RPA) is a relatively new isothermal methodology for amplifying DNA. RPA is similar to traditional PCR in that it produces an amplicon that is defined by the annealing of two opposing oligonucleotide primers.
Richards, Stephen M, Mitchell, Kieren J, Tobler, Raymond, Cooper, Alan   +3 more
openaire   +1 more source

Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis [PDF]

Journal of Clinical Microbiology, 2012
ABSTRACT Several real-time PCR approaches to develop field detection for Francisella tularensis , the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant ...
Euler, Milena, Wang, Yongjie, Otto, Peter, Tomaso, Herbert, Escudero, Raquel, Anda, Pedro, Hufert, Frank T, Weidmann, Manfred   +7 more
openaire   +4 more sources

Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism

Advanced Science, EarlyView.
The study demonstrated that connexin43 (Cx43) knockout caused arrhythmic phenotype and decreased proline content in vitro and in vivo. Mechanistically, Cx43 interacts with the amino acid transporter SNAT2 (sodium‐dependent neutral amino acid transporter), and its deficiency disrupts proline transport and metabolism.
Hangying Ying, Hangping Fan, Yunhe Wang, Ruhong Jiang, Dongsheng Cai, Hui Cheng, Hao Wang, Chenyang Jiang, Ping Liang   +8 more
wiley   +1 more source

Development of recombinase polymerase amplification detection method for Listeria monocytogenes in food

Zhongguo shipin weisheng zazhi, 2021
ObjectiveTo establish a recombinase polymerase amplification (RPA) method for the detection of Listeria monocytogenes (L. monocytogenes) in food.MethodsBased on the hlyA gene of L.
HE Jie, ZHU Chao, HUANG Shanshan, WU Qiaowei, SUN Ping, LAO Huajun   +5 more
doaj   +1 more source

Development of a Recombinase Polymerase Amplification-Coupled CRISPR/Cas12a Platform for Rapid Detection of Antimicrobial-Resistant Genes in Carbapenem-Resistant Enterobacterales

Biosensors
The worldwide spread of carbapenemase-producing Enterobacterales (CPE) represents a significant threat owing to the high mortality and morbidity rates.
Ji Woo Yang, Heesu Kim, Lee-Sang Hyeon, J. Yoo, Sangrim Kang   +4 more
semanticscholar   +1 more source

Chaperone‐Mediated Autophagic Degradation of USP9X in Macrophages Exacerbates Postmyocardial Infarction Inflammation and Cardiac Dysfunction

Advanced Science, EarlyView.
This study demonstrates that inflammatory stimuli induce the acetylation‐triggered, chaperone‐mediated autophagic degradation of ubiquitin‐specific peptidase 9 X‐linked (USP9X) in macrophages. USP9X acts as a macrophage “inflammation switch” after myocardial infarction (MI). USP9X loss destabilizes tumor necrosis factor receptor‐associated factor (TRAF)
Biqing Wang, Xiangheng Cai, Mengqi Li, Xue Liu, Junhui Xue, Ye Liu, Ding Ai, Xinyang Hu   +7 more
wiley   +1 more source

Fully integrated sample-in-answer-out platform for viral detection using digital reverse transcription recombinase polymerase amplification (dRT-RPA).

Biosensors & bioelectronics, 2023
Recombinase polymerase amplification (RPA) is one of the most promising diagnostic methods for pathogen detection, owing to the simplified isothermal amplification technique.
Islam Seder, Rodrigo H. Coronel-Tellez, S. H. Helalat, Yi Sun   +3 more
semanticscholar   +1 more source