Results 101 to 110 of about 17,679 (284)

DLC2 modulates angiogenic responses in vascular endothelial cells by regulating cell attachment and migration. [PDF]

, 2010
Deleted in liver cancer 1 (DLC1) is a RhoGTPase activation protein-containing tumor suppressor that associates with various types of cancer. Although DLC2 shares a similar domain structure with that of DLC1, the function of DLC2 is not well characterized.
Chen, N-T, Liao, Y-C, Lin, Y, Lo, SH, Shih, Y-P, Xue, L   +5 more
core   +3 more sources

Boolean Recombinase-Based Devices [PDF]

, 2019
This paper relates to a central problem in synthetic biology, which is that of designing Recombinase-based biological devices by matching a functional specification expressed as a Boolean function. This task is challenging as exploring the space of possibilities is typically unfeasible, and therefore many non-trivial design alternatives remain ...
Pérution-Kihli, Guillaume, Guiziou, Sarah, Ulliana, Federico, Leclère, Michel, Bonnet, Jérôme   +4 more
openaire   +2 more sources

Generation of chickens expressing Cre recombinase [PDF]

Transgenic Research, 2016
Cre recombinase has been extensively used for genome engineering in transgenic mice yet its use in other species has been more limited. Here we describe the generation of transgenic chickens expressing Cre recombinase. Green fluorescent protein (GFP)-positive chicken primordial germ cells were stably transfected with β-actin-Cre-recombinase using ...
Leighton, Philip A., Pedersen, Darlene, Ching, Kathryn, Collarini, Ellen J., Izquierdo, Shelley, Jacob, Roy, van de Lavoir, Marie-Cecile   +6 more
openaire   +2 more sources

Palmitoylation‐Mediated Ubiquitination of SRPK1 Regulates Ferroptosis in High‐Fat‐Induced Erectile Dysfunction

Advanced Science, EarlyView.
Elevated exogenous palmitic acid promotes the S‐palmitoylation of SRPK1 in endothelial cells, a dynamic process governed by ZDHHC24 and APT1. This post‐translational modification strengthens the interaction between SRPK1 and the E3 ubiquitin ligase MIB1, thereby facilitating the proteasomal degradation of SRPK1.
Xiao‐Hui Tan, Ke‐Fan Li, Yi‐Ming Yuan, Man‐Cheng Xia, Fang‐Zhou Zhao, Hong‐Gang Ying, Zhuo Zhou, Peng‐Chao Gao, Guo‐Qing Xie, Xue‐Song Li, Hui Jiang, Rui‐Li Guan   +11 more
wiley   +1 more source

Myeloid p38 activation maintains macrophage–liver crosstalk and BAT thermogenesis through IL‐12–FGF21 axis

Hepatology, EarlyView., 2022
Physiological activation of myeloid p38 controls macrophage IL‐12 production and crosstalk to the liver by modulating hepatic FGF21, and subsequently, brown adipose tissue thermogenesis during obesity Abstract Obesity features excessive fat accumulation in several body tissues and induces a state of chronic low‐grade inflammation that contributes to ...
María Crespo, Ivana Nikolic, Alfonso Mora, Elena Rodríguez, Luis Leiva‐Vega, Aránzazu Pintor‐Chocano, Daniel Horrillo, Lourdes Hernández‐Cosido, Jorge L. Torres, Eva Novoa, Rubén Nogueiras, Gema Medina‐Gómez, Miguel Marcos, Magdalena Leiva, Guadalupe Sabio   +14 more
wiley   +1 more source

Coordinated Binding of Single-Stranded and Double-Stranded DNA by UvsX Recombinase. [PDF]

PLoS ONE, 2013
Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/Rad51 family perform the central catalytic role in this process.
Robyn L Maher, Scott W Morrical
doaj   +1 more source

The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation [PDF]

, 2014
We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or
A Maas, A Maas, Alexander W. Tucker, Andrew N. Rycroft, B Zhan, Brendan W. Wren, C O'Neill, Denise M. Soares-Bazzolli, Duncan J. Maskell, EA Izano, FF Buettner, G Li, Glenn Francis Browning, H Maughan, I Jacobsen, I Jacobsen, Janine T. Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, JT Bossé, L Lin, LM Mullen, M Copass, M Ramjeet, N Baltes, N Baltes, N Baltes, N Baltes, N Baltes, Paul R. Langford, PJ Blackall, R Jansen, RJ Redfield, RM Jones, RV Joshi, SJ Foote, T Ali, TJ Inzana, W Oswald, W Tonpitak, W Tonpitak, WW Fung, Yanwen Li, Z Xu   +46 more
core   +5 more sources

Programmable Klebsiella pneumoniae Phage Tropism Enabled by Scalable Receptor‐Binding Protein Mining and Modular Assembly

Advanced Science, EarlyView.
This work introduces a scalable, data‐driven strategy that turns the vast sequence diversity of phage receptor‐binding proteins into modular tools for engineering customizable phages. By clustering and experimentally validating diverse RBPs, the authors build an accurate map linking sequence to host specificity and create plug‐and‐play modules that ...
Shisong Jing, Yiyao Song, Xianbiao Bi, Yuqin Song, Dawei Wei, Jiangqing Huang, Yuan Zeng, Gang Zhang, Rong Zhang, Chao Wang, Jie Feng   +10 more
wiley   +1 more source

ALK1 controls hepatic vessel formation, angiodiversity, and angiocrine functions in hereditary hemorrhagic telangiectasia of the liver

Hepatology, EarlyView., 2022
Hepatic endothelial Alk1 signaling protects from development of vascular malformations while maintaining organ‐specific endothelial differentiation and angiocrine portmanteau of the names Wingless and Int‐1 signaling. Abstract Background and Aims In hereditary hemorrhagic telangiectasia (HHT), severe liver vascular malformations are associated with ...
Christian David Schmid, Victor Olsavszky, Manuel Reinhart, Vanessa Weyer, Felix A. Trogisch, Carsten Sticht, Manuel Winkler, Sina W. Kürschner, Johannes Hoffmann, Roxana Ola, Theresa Staniczek, Joerg Heineke, Beate K. Straub, Jens Mittler, Kai Schledzewski, Peter ten Dijke, Karsten Richter, Steven Dooley, Cyrill Géraud, Sergij Goerdt, Philipp‐Sebastian Koch   +20 more
wiley   +1 more source

Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods [PDF]

, 2018
Exogenous pathway optimization and chassis engineering are two crucial methods for heterologous pathway expression. The two methods are normally carried out step-wise and in a trial-and-error manner.
Auer, Manfred, Cai, Yizhi, Dai, Junbiao, French, Christopher, Liu, Longying, Liu, Wei, Luo, Zhouqing, Marles-Wright, Jon, Perez Pi, Irene, Pham, Nhan T., Shen, Yue, Tuck, Laura, Wang, Yun   +12 more
core   +1 more source