Results 221 to 230 of about 17,679 (284)

Efficient high-precision transgene knock-in by Recombinases (Redα/β)-enhanced DNA integration-CRISPR-Cas9 (RED-CRISPR). [PDF]

Nat Commun
Li W, Liu S, Fang X, Zou J, Jiang Q, Min X, Zhu X, Cao Y, Gao X, Han W, Azhar M, Xing X, Li F, Zhang Y, Liu H, Cheng L, Wang C, Bao J.   +17 more
europepmc   +1 more source

Making Molecular Diagnostics Faster

International Journal of Laboratory Hematology, Volume 48, Issue 2, Page 272-280, April 2026.
ABSTRACT Background Over the past 40 years, molecular diagnostic methods have evolved from multi‐step, time‐consuming protocols towards either rapid targeted tests or expansive, massively parallel testing. Aims Here we consider the speed limits of targeted molecular diagnostics, considering the three sequential required steps: nucleic acid preparation,
Carl T. Wittwer, Luming Zhou, Felix Ye, Adam Millington, Adrian de Cola, Noriko Kusukawa   +5 more
wiley   +1 more source

Prophage φEr670 and Genomic Island GI_Er147 as Carriers of Resistance Genes in <i>Erysipelothrix rhusiopathiae</i> Strains. [PDF]

Int J Mol Sci
Dec M, Zomer AL, Broekhuizen-Stins MJ, Urban-Chmiel R.   +3 more
europepmc   +1 more source

CRISPR‐Enhanced RAA‐SHERLOCK Assay for Point‐of‐Care Detection of Cyprinid Herpesvirus‐3: Development, Validation and Clinical Application

Journal of Fish Diseases, Volume 49, Issue 4, April 2026.
ABSTRACT Koi herpesvirus disease (KHVD), caused by Cyprinid herpesvirus‐3 (CyHV‐3), poses a significant threat to global aquaculture due to its high mortality rates and economic impact. Current diagnostic methods, such as PCR, are limited by equipment dependency and procedural complexity, hindering point‐of‐care (POC) applications.
Hatem Soliman, Naveed Akram, Mona Saleh
wiley   +1 more source

Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli. [PDF]

J Biol Eng
Xie C, Gan J, Zhang Y, Yu W, Li R, Yu D, Liu Y, Zheng SG, Qi G.   +8 more
europepmc   +1 more source

Porphyromonas gingivalis–Derived Virulence Lipids Accelerate Osteoclastogenesis Independently of High Mobility Group Box Protein‐1 Canonical Signaling

Molecular Oral Microbiology, Volume 41, Issue 2, Page 85-93, April 2026.
ABSTRACT Periodontal bacterial pathogen‐associated molecular patterns (PAMPs) and damage‐associated molecular patterns (DAMPs) accelerate inflammatory osteoclastogenesis, resulting in alveolar bone loss. The core PAMP and DAMP prototype molecules are periodontal bacterium Porphyromonas gingivalis–derived virulence lipids, for example, phosphoglycerol ...
Chiaki Yamada, Gang Peng, James A. Johnson Jr., Amilia Nusbaum, Natasha Sanz, Hawra AlQallaf, Frank Nichols, Alexandru Movila   +7 more
wiley   +1 more source

Synthetic rewriting technologies in mammalian cells. [PDF]

Nat Commun
Wang Y, Cui Y, Zhao GR, Wu Y, Yuan YJ.
europepmc   +1 more source

ZNF384‐Driven Fibulin‐1 Exacerbates Vascular Stiffness via TGF‐β/Smad3‐Mediated Senescence and Fibrosis

The FASEB Journal, Volume 40, Issue 6, 31 March 2026.
This article elucidates the regulatory molecular mechanisms of Fibulin‐1 (Fbln1) in vascular stiffness and explores potential therapeutic interventions. Zinc Finger Protein 384 (ZNF384) binds to the Fbln1 promoter, driving its expression. Fbln1 activates the TGF‐β/Smad3 axis, promoting vascular smooth muscle cell (VSMC) senescence and collagen ...
Dan Yan, Tianyi ji, Xiaolu Liang, Mandi Luo, Yi Huang, Pengcheng Luo, Zhen Yang, Le Zhang, Tao Li, Yong Ping Bai, Cuntai Zhang, Lei Ruan   +11 more
wiley   +1 more source

There and turn back again: the application of phage serine integrases in eukaryotic systems. [PDF]

Front Bioeng Biotechnol
Sales TT, de Oliveira MA, Florentino LH, Lima RN, Rech E.   +4 more
europepmc   +1 more source

Ketone Body Supplementation Exerts Renoprotective Effects Against Adenine‐Induced Kidney Injury via OXCT1‐Mediated Ketolysis in Mice

The FASEB Journal, Volume 40, Issue 6, 31 March 2026.
1,3‐Butanediol is a ketone body precursor that is directly converted into the ketone bodies, β‐hydroxybutyrate (BHB), in the liver. When renal ketolysis, regulated by 3‐oxoacid CoA‐transferase 1 (OXCT1), is preserved, BHB supplementation exerts renoprotective effects.
Shoji Omachi, Sho Sugahara, Junya Horiguchi, Mako Yasuda‐Yamahara, Shogo Kuwagata, Kosuke Yamahara, Yuki Tanaka‐Sasaki, Shogo Ida, Tetsuro Kusaba, Benjamin D. Humphreys, Kenji Kufukihara, Jin Nakahara, Masami Chin‐Kanasaki, Shinji Kume   +13 more
wiley   +1 more source