Identification of the Integration/Excision Module and Regulatory Elements Involved in the Mobility of IME8, an Integrative and Mobilizable Element From Mosquitocidal Lysinibacillus sphaericus. [PDF]
Microb BiotechnolHu Y +5 more
europepmc +1 more source
A chemically inducible, leakless Cre recombinase by split-protein-based efficient and enhanced degradation (SPEED). [PDF]
Commun BiolCai D +4 more
europepmc +1 more source
Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2018 Bacterial genomes encode numerous cryptic biosynthetic gene clusters (BGCs) that represent a largely untapped source of drugs or pesticides. Mining of the cryptic products is limited by the unavailability of streamlined genetic tools in native producers.
Rolf Müller, Youming Zhang, Yuezhong Li
exaly +3 more sources
Diversity in the serine recombinases [PDF]
Molecular Microbiology, 2002 SummaryMost site‐specific recombinases fall into one of two families, based on evolutionary and mechanistic relatedness. These are the tyrosine recombinases orλintegrase family and the serine recombinases or resolvase/invertase family. The tyrosine recombinases are structurally diverse and functionally versatile and include integrases, resolvases ...
Margaret C. M. Smith
exaly +3 more sources
Multiple new site-specific recombinases for use in manipulating animal genomes
Proceedings of the National Academy of Sciences of the United States of America, 2011 Site-specific recombinases have been used for two decades to manipulate the structure of animal genomes in highly predictable ways and have become major research tools.
Gerald M. Rubin +2 more
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Cre Recombinase and Other Tyrosine Recombinases
Chemical Reviews, 2016Tyrosine-type site-specific recombinases (T-SSRs) have opened new avenues for the predictable modification of genomes as they enable precise genome editing in heterologous hosts. These enzymes are ubiquitous in eubacteria, prevalent in archaea and temperate phages, present in certain yeast strains, but barely found in higher eukaryotes.
Gretchen, Meinke +4 more
openaire +2 more sources
Conservation of Structure and Mechanism between Eukaryotic Topoisomerase I and Site-Specific Recombinases [PDF]
Cell, 1998 Vaccinia DNA topoisomerase breaks and rejoins DNA strands through a DNA-(3′-phosphotyrosyl)-enzyme intermediate. A C-terminal catalytic domain, Topo(81–314), suffices for transesterification chemistry.
Stewart Shuman
exaly +2 more sources
Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
Methods in Molecular Biology, 2017Recombinase-mediated cassette exchange, or RMCE, is a genome engineering tool that can be used to swap DNA fragments of interest between two DNA molecules. In a variation of RMCE, called dual RMCE, the exchange of DNA fragments is mediated by two recombinases in contrast to one recombinase in the classic RMCE reaction.
Rachelle P Anderson
exaly +3 more sources
Catalysis by site-specific recombinases
Trends in Genetics, 1992Site-specific recombination reactions bring about controlled rearrangements of DNA molecules by cutting the DNA at precise points and rejoining the ends to new partners. The recombinases that catalyse these reactions can be grouped into two families by amino acid sequence homology.
W M, Stark, M R, Boocock, D J, Sherratt
openaire +2 more sources