Cdc14 phosphatase counteracts Cdk-dependent Dna2 phosphorylation to inhibit resection during recombinational DNA repair [PDF]
Nature Communications, 2023 Cyclin-dependent kinase (Cdk) stimulates resection of DNA double-strand breaks ends to generate single-stranded DNA (ssDNA) needed for recombinational DNA repair.
Adrián Campos +7 more
doaj +2 more sources
Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading [PDF]
Nature CommunicationsDNA damage occurs in all cells and must be repaired to maintain genome integrity. Many DNA lesions are targeted for removal by repair systems that excise the damage, thereby generating a temporary single-strand discontinuity in the chromosome.
Charles Winterhalter +3 more
doaj +3 more sources
Spatiotemporal characterization of single-stranded DNA Intermediates after UV Irradiation: I: Post-replication gaps formed during slow growth. [PDF]
PLoS GeneticsWhen E. coli cells are UV irradiated, replisome encounters with some DNA lesions lead to lesion skipping and formation of ssDNA gaps. These gaps are protected by SSB and repaired through the RecFOR recombinational DNA repair pathway.
Nischal Sharma +7 more
doaj +2 more sources
Tn-seq of Thermus thermophilus Genome Reveals Unexpected Tolerance to Insertions in Bacterial Common Essential Genes. [PDF]
MicrobiologyopenTn‐seq analysis of Thermus thermophilus genome reveals insertions in almost all genes, this is, there is no clear discrimination of essential genes. A similar result has not been reported in other Tn‐seq analysis of bacterial genomes so far. The polyploidy of the Thermus genome could explain the apparent non‐essentiality of key genes.
Gómez-Campo CL +6 more
europepmc +2 more sources
PP4 phosphatase cooperates in recombinational DNA repair by enhancing double-strand break end resection [PDF]
Nucleic Acids Research, 2019 Facundo Ramos +2 more
exaly +2 more sources
TOPBP1 takes RADical command in recombinational DNA repair [PDF]
Journal of Cell Biology, 2016 Marcus B Smolka, Smolka Marcus B
exaly +2 more sources
Dynamic Processing of Displacement Loops during Recombinational DNA Repair [PDF]
Molecular Cell, 2019 Aurèle Piazza +2 more
exaly +2 more sources
DNA-RNA hybrids at DSBs interfere with repair by homologous recombination
eLife, 2021 DNA double-strand breaks (DSBs) are the most harmful DNA lesions and their repair is crucial for cell viability and genome integrity. The readout of DSB repair may depend on whether DSBs occur at transcribed versus non-transcribed regions.
Pedro Ortega +4 more
doaj +1 more source
A novel motif of Rad51 serves as an interaction hub for recombination auxiliary factors
eLife, 2021 Homologous recombination (HR) is essential for maintaining genome stability. Although Rad51 is the key protein that drives HR, multiple auxiliary factors interact with Rad51 to potentiate its activity.
Negar Afshar +5 more
doaj +1 more source
Cdk1 targets Srs2 to complete synthesis-dependent strand annealing and to promote recombinational repair. [PDF]
PLoS Genetics, 2010 Cdk1 kinase phosphorylates budding yeast Srs2, a member of UvrD protein family, displays both DNA translocation and DNA unwinding activities in vitro. Srs2 prevents homologous recombination by dismantling Rad51 filaments and is also required for double ...
Marco Saponaro +6 more
doaj +1 more source