Results 71 to 80 of about 9,223,247 (288)

The use of reconstructed human epidermis for skin absorption testing

, 2022
S.161-187A formal validation study was performed, in order to investigate whether the commercially available reconstructed human epidermis (RHE) models, EPISKIN (R), EpiDerm (TM) and SkinEthic (R), are suitable for in vitro skin absorption testing.
Kamp, H., Schäfer, U., Kaca, M., Bock, U., Haltner-Ukomadu, E., Vuia, A., Düsing, H.-J., Netzlaff, F., Niedorf, F., Mehling, A., Schreiber, S., Hoffmann, C., Kersen, S., Schmidt, E., Kietzmann, M., Müller-Goymann, C., Lehr, C.-M., Diembeck, W., Gamer, A., Spielmann, H., Krächter, H.-U., Liebsch, M., Korting, H.C., Weimer, M., Rübbelke, M.K., Schäfer-Korting, M.   +25 more
core   +1 more source

Esterase Activity and Intracellular Localization in Reconstructed Human Epidermal Cultured Skin Models [PDF]

, 2014
Background : Reconstructed human epidermal culture skin models have been developed for cosmetic and pharmaceutical research. Objective : This study evaluated the total and carboxyl esterase activities (i.e., Km and Vmax, respectively) and localization in
橋本, フミ惠, 徳留, 嘉寛, 片柳, 美姿   +2 more
core   +1 more source

Spatially Controlled Embedded Bioprinting of Prevascularized Spheroids‐Containing Biopatch Enables Functional Skin Regeneration

Advanced Healthcare Materials, EarlyView.
A spatially controlled embedded bioprinting strategy enables precise engineering of prevascularized multicellular spheroids within skin‐derived dECM bioinks. Optimized spheroid size, composition, and spacing promote vascular maturation, paracrine signaling, and tissue integration, resulting in enhanced angiogenesis, blood perfusion, and functional skin
Minjun Ahn, Gyu Tae Park, Dongjun Lee, Sik Yoon, Sae‐Ock Oh, Jae Ho Kim, Byoung Soo Kim   +6 more
wiley   +1 more source

A new reconstructed human epidermis for in vitro skin irritation testing

Toxicology in Vitro, 2017
Different models of reconstructed human epidermis (RHE) are currently validated to assess skin irritation in vitro and ultimately to the animal replacement of the Draize test. The development of a new RHE model is a challenge for many laboratories, representing a potential gain of autonomy and improvement of technological knowledge.
Tatiana do Nascimento, Pedrosa, Carolina Motter, Catarino, Paula Comune, Pennacchi, Sílvia Romano de, Assis, Fabrícia, Gimenes, Márcia Edilaine Lopes, Consolaro, Silvia Berlanga de Moraes, Barros, Silvya Stuchi, Maria-Engler   +7 more
openaire   +2 more sources

Model Of Human Epidermis Reconstructed In Vitro With Keratinocytes And Melanocytes On Dead De-epidermized Human Dermis. [PDF]

, 2015
Recent progress in the field of epithelial culture techniques has allowed the development of culture systems in which the reconstructed epidermis presents characteristics of morphological differentiation similar to those seen in vivo.
Issa, Cláudia Maria Bernardino Magro, Souto, Luís Ricardo Martinhão, Rehder, Jussara, Puzzi, Maria Beatriz   +3 more
core  

Advancing Human Skin Equivalents: The Crucial Role of Neurovascular Integration

Advanced Healthcare Materials, EarlyView.
This review discusses the importance of integrating vascular and peripheral nerve systems into human skin equivalents (HSEs) to better recapitulate native skin physiology. Recent advances in vascularized, innervated, and neurovascularized HSEs are highlighted, together with emerging bioengineering strategies, current challenges, and future ...
Hao Wu, Lorenzo Moroni, Nicolas Lebonvallet, Vincent Flacher, Paul Wieringa   +4 more
wiley   +1 more source

Self‐Healing Hydrogel‐Enabled Modular Assembly of Bilayered Skin Construct for Hair Follicle Regeneration

Advanced Healthcare Materials, EarlyView.
Self‐healing assembly of two cell‐laden hydrogel layers creates a biomimetic skin construct that restores both dermal and epidermal functions, resulting in accelerated wound healing and enhanced hair follicle regeneration in a full‐thickness skin wound model. ABSTRACT Alopecia remains a pervasive clinical challenge, largely owing to the limited ability
JaeWook Park, Su Yong Kim, MinSu Jeong, Beom Joon Kim, Shin Hyuk Kang, Won‐Gun Koh   +5 more
wiley   +1 more source

NeuroVisio SyncPatch: A Skin‐Conformal Multimodal EMG–Motion Sensing Platform for Quantitative Neuromuscular Rehabilitation and Performance Monitoring

Advanced Healthcare Materials, EarlyView.
NeuroVisio SyncPatch integrates skin‐conformal electromyography (EMG) electrodes with camera‐tracked markers to jointly assess muscle activity and three‐dimensional knee kinematics during rehabilitation. Parallel feedback pathways provide real‐time kinematic guidance during movement and post‐trial neuromuscular feedback.
Bohyung Choi, Yoo‐Jin Jun, Heewon Choee, Kun‐Do Lee, Heesoo Kim, Yu Seok Hwang, Choong Yeon Kim, Seongok Chae, Juhyun Lee, Minkyu Je, Jae‐Woong Jeong, Hyung‐Soon Park   +11 more
wiley   +1 more source

Freeze-Fracture Electron Microscopy of In Vitro Reconstructed Human Epidermis

Journal of Investigative Dermatology, 1990
Epidermis has been reconstructed in vitro by seeding human keratinocytes on a human dermal substrate in an air-exposed culture. The end product has been examined by freeze-fracture electron microscopy, transmission electron microscopy (TEM) of thin sections, light microscopy, and lipid analysis using thin-layer chromatography.
Boddé, Harry E, Holman, Bert, Spies, Ferry, Weerheim, Arij, Kempenaar, Johanna, Mommaas, Mieke, Ponec, Maria   +6 more
openaire   +2 more sources

Protection of normal human reconstructed epidermis from UV by catalase overexpression [PDF]

Cancer Gene Therapy, 2006
Reactive oxygen species (ROS) generated by ultraviolet (UV) irradiation are counterbalanced by endogenous antioxidant systems. To test the hypothesis of a novel photoprotective approach, we irradiated epidermis reconstructed with normal human keratinocytes overexpressing sustainably lentivirus-mediated catalase (CAT), copper/zinc superoxide dismutase ...
H R, Rezvani, M, Cario-André, C, Pain, C, Ged, H, deVerneuil, A, Taïeb   +5 more
openaire   +2 more sources