Results 51 to 60 of about 7,690,139 (265)

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter

, 2013
This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions.
De Vuyst, Evelyne, Charlier, Céline, Giltaire, Séverine, De Glas, Valérie, De Rouvroit, Catherine Lambert, Poumay, Yves   +5 more
openaire   +3 more sources

A new reconstructed human epidermis for in vitro skin irritation testing

Toxicology in Vitro, 2017
Different models of reconstructed human epidermis (RHE) are currently validated to assess skin irritation in vitro and ultimately to the animal replacement of the Draize test. The development of a new RHE model is a challenge for many laboratories, representing a potential gain of autonomy and improvement of technological knowledge.
Tatiana do Nascimento, Pedrosa, Carolina Motter, Catarino, Paula Comune, Pennacchi, Sílvia Romano de, Assis, Fabrícia, Gimenes, Márcia Edilaine Lopes, Consolaro, Silvia Berlanga de Moraes, Barros, Silvya Stuchi, Maria-Engler   +7 more
openaire   +2 more sources

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

Implementation, availability and regulatory status of an OECD accepted Reconstructed Human Epidermis model in Brazil

Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia, 2018
Introduction: In 2014, Brazil has joined the growing list of countries to ban cosmetic products from being tested on animal models. The new legislation comes into force in 2019.
Rodrigo De Vecchi, Vanja Dakic, Guilherme Mattos, Anne-Sophie Rigaudeau, Veronica Oliveira, Cristina Garcia, Nathalie Alépée, José Cotovio, Charbel Bouez   +8 more
doaj   +7 more sources

The use of reconstructed human epidermis for skin absorption testing

, 2022
S.161-187A formal validation study was performed, in order to investigate whether the commercially available reconstructed human epidermis (RHE) models, EPISKIN (R), EpiDerm (TM) and SkinEthic (R), are suitable for in vitro skin absorption testing.
Kamp, H., Schäfer, U., Kaca, M., Bock, U., Haltner-Ukomadu, E., Vuia, A., Düsing, H.-J., Netzlaff, F., Niedorf, F., Mehling, A., Schreiber, S., Hoffmann, C., Kersen, S., Schmidt, E., Kietzmann, M., Müller-Goymann, C., Lehr, C.-M., Diembeck, W., Gamer, A., Spielmann, H., Krächter, H.-U., Liebsch, M., Korting, H.C., Weimer, M., Rübbelke, M.K., Schäfer-Korting, M.   +25 more
core   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models

Nanomaterials, 2018
Silver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin.
Sylvia G. Lehmann, Benjamin Gilbert, Thierry GG Maffeis, Alexei Grichine, Isabelle Pignot-Paintrand, Simon Clavaguera, Walid Rachidi, Michel Seve, Laurent Charlet   +8 more
doaj   +1 more source

Rare‐Earth–Functionalized Carbon Dot Hydrogels as Mechanically Robust Antibacterial Wound Dressings

Advanced Healthcare Materials, EarlyView.
The schematic depicts a dynamically cross‐linked PVA hydrogel network in which reversible borate ester bonds and hydrogen‐bonding interactions integrate lanthanum‐doped carbon dots (La‐CDs) and dipotassium glycyrrhizinate (DPG) to form a mechanically robust, multifunctional matrix.
Shih‐Han Tsai, Ramadhass Keerthika Devi, Muthusankar Ganesan, Guo‐Ping Chang Chien, Chun Che Lin   +4 more
wiley   +1 more source

Spatially Controlled Embedded Bioprinting of Prevascularized Spheroids‐Containing Biopatch Enables Functional Skin Regeneration

Advanced Healthcare Materials, EarlyView.
A spatially controlled embedded bioprinting strategy enables precise engineering of prevascularized multicellular spheroids within skin‐derived dECM bioinks. Optimized spheroid size, composition, and spacing promote vascular maturation, paracrine signaling, and tissue integration, resulting in enhanced angiogenesis, blood perfusion, and functional skin
Minjun Ahn, Gyu Tae Park, Dongjun Lee, Sik Yoon, Sae‐Ock Oh, Jae Ho Kim, Byoung Soo Kim   +6 more
wiley   +1 more source