Results 11 to 20 of about 976 (165)

Development of a Propidium Monoazide-Based Viability Quantitative PCR Assay for Red Sea Bream Iridovirus Detection. [PDF]

Int J Mol Sci, 2023
Red sea bream iridovirus (RSIV) is an important aquatic virus that causes high mortality in marine fish. RSIV infection mainly spreads through horizontal transmission via seawater, and its early detection could help prevent disease outbreaks. Although quantitative PCR (qPCR) is a sensitive and rapid method for detecting RSIV, it cannot differentiate ...
Kim KH, Kang G, Woo WS, Sohn MY, Son HJ, Park CI.   +5 more
europepmc   +5 more sources

Transcription program of red sea bream iridovirus as revealed by DNA microarrays. [PDF]

J Virol, 2005
ABSTRACT Red sea bream iridovirus (RSIV) has been identified as the causative agent of a serious disease in red sea bream and at least 30 other marine fish species. We developed a viral DNA microarray containing 92 putative open reading frames of RSIV to monitor the viral gene transcription program over the time course of an in ...
Lua DT, Yasuike M, Hirono I, Aoki T.
europepmc   +6 more sources

Megalocytiviruses [PDF]

Viruses, 2012
The genus Megalocytivirus, represented by red sea bream iridovirus (RSIV), the first identified and one of the best characterized megalocytiviruses, Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus, and numerous other ...
Kazuhiro Nakajima, Jun Kurita
doaj   +4 more sources

A New Cell Line Derived from the Caudal Fin of the Dwarf Gourami (Trichogaster lalius) and Its Susceptibility to Fish Viruses [PDF]

Biology, 2023
The detection of megalocytiviruses, especially the infectious spleen and kidney necrosis virus (ISKNV), in ornamental fish has increased with the rapid growth of the ornamental fish industry.
Ye-Jin Jeong, Kwang-Il Kim
doaj   +2 more sources

Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction [PDF]

Diseases of Aquatic Organisms, 1998
A simple and sensitive polymerase chain reaction (PCR) based assay is described for detection of the red sea bream iridovirus (RSIV) in infected fish. The assay involves amplification of a portion of the ribonucleotide reductase small subunit (RNRS) gene of the virus from DNA isolated from the spleen. The system was tested on red sea bream following an
S, Oshima, J, Hata, N, Hirasawa, T, Ohtaka, I, Hirono, T, Aoki, S, Yamashita   +6 more
openaire   +4 more sources

Development and Validation of a Quantitative Polymerase Chain Reaction Assay for the Detection of Red Sea Bream Iridovirus

Fishes, 2022
The analytical and diagnostic performances of methods for detecting red sea bream iridovirus (RSIV), which infects marine fish, have not been evaluated. As disease management and transmission control depend on early and reliable pathogen detection, rapid
Kyung-Ho Kim, Kwang-Min Choi, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Dongbin Yun, Do-Hyung Kim, Chan-Il Park   +8 more
doaj   +3 more sources

MiR-181a Negatively Regulates Claudin-3 to Facilitate Lateolabrax maculatus Iridovirus Replication in Lateolabrax maculatus Astroglia Cells [PDF]

Viruses
Lateolabrax maculatus iridovirus (LMIV) is a variant strain of red sea bream iridovirus (RSIV), causing serious economic losses in aquaculture. Claudins (CLDNs) are major components of tight junctions (TJs) forming an important line of defense against ...
Yanping Ma, Jingjing Xu, Le Hao, Gang Wang, Wen Huang, Zhenxing Liu   +5 more
doaj   +2 more sources

Establishing environmental DNA and RNA protocols for the simultaneous detection of fish viruses from seawater

Environmental DNA
Aquatic viruses are major threats to global aquacultural productivity. While conventional diagnostic methods for disease investigation are laborious, time‐consuming, and often involve the sacrifice of animals, environmental DNA and RNA (eDNA/eRNA) tools ...
Yin Cheong Aden Ip, Jing Chen, Li Ying Tan, Clara Lau, Ying Hui Chan, Ravendrakumar Shanmugavelu Balasubramaniam, Wan Yen Jovinc Wong, Kaitlyn Ng, Zi Yan Brian Tan, Charlene Judith Fernandez, Siow Foong Chang, Him Hoo Yap   +11 more
doaj   +2 more sources

Epidemiological investigation on diseases of Larimichthys crocea in Ningbo culture area [PDF]

Frontiers in Cellular and Infection Microbiology
IntroductionDue to the high-density farming of Larimichthys crocea over the years, diseases caused by pathogens such as bacteria, viruses, and parasites frequently occur in Ningbo, posing a huge threat and challenge to the sustainable and healthy ...
Shengwei Xu, Shengwei Xu, Shengwei Xu, Shengwei Xu, Mingfeng Ge, Mingfeng Ge, Mingfeng Ge, Mingfeng Ge, Juan Feng, Xinxian Wei, Honglian Tan, Zheng Liang, Guixiang Tong   +12 more
doaj   +2 more sources

Risk assessment of wild fish as environmental sources of red sea bream iridovirus (RSIV) outbreaks in aquaculture [PDF]

Diseases of Aquatic Organisms
Red sea bream iridovirus (RSIV) causes substantial economic damage to aquaculture. In the present study, RSIV in wild fish near aquaculture installations was surveyed to evaluate the risk of wild fish being an infection source for RSIV outbreaks in cultured fish. In total, 1102 wild fish, consisting of 44 species, were captured from 2 aquaculture areas
Yasuhiko, Kawato, Kaori, Mizuno, Shogo, Harakawa, Yuzo, Takada, Yusaku, Yoshihara, Hidemasa, Kawakami, Takafumi, Ito   +6 more
core   +5 more sources