Results 31 to 40 of about 171,869 (285)

Reduced folate carrier protein expression in osteosarcoma [PDF]

Cancer, 2003
AbstractBACKGROUNDHigh‐dose methotrexate (MTX) is an important component of current protocols for the treatment of osteosarcoma. Although MTX uptake proceeds primarily through the reduced folate carrier (RFC) protein and efflux occurs via multidrug resistance protein 1 (MRP1), RFC protein expression in osteosarcoma remains unexamined.METHODSRFC and ...
Ilan, Ifergan, Issac, Meller, Josefin, Issakov, Yehuda G, Assaraf   +3 more
openaire   +2 more sources

Stereoselectivity of the reduced folate carrier in Caco‐2 cells

Chirality, 2005
AbstractStereoselectivity of the human reduced folate carrier (RFC1) was examined in Caco‐2 cells using methotrexate (l‐amethopterin or l‐MTX) and its antipode (d‐amethopterin or d‐MTX) as model substrates. The initial uptake rate of folic acid (FA) was concentration dependent, with a Km value of approximately 0.6 μM. The Eadie–Hofstee plot of the RFC1‐
Tomoya, Narawa, Rikako, Shimizu, Shuhei, Takano, Yasuyuki, Tsuda, Kiyoko, Ono, Hideo, Yamada, Tomoo, Itoh   +6 more
openaire   +5 more sources

Folate transporter dynamics and therapy with classic and tumor-targeted antifolates

Scientific Reports, 2021
There are three major folate uptake systems in human tissues and tumors, including the reduced folate carrier (RFC), folate receptors (FRs) and proton-coupled folate transporter (PCFT). We studied the functional interrelationships among these systems for
Carrie O’Connor, Adrianne Wallace-Povirk, Changwen Ning, Josephine Frühauf, Nian Tong, Aleem Gangjee, Larry H. Matherly, Zhanjun Hou   +7 more
doaj   +1 more source

Paradoxical impact of two folate receptors, FRα and RFC, in ovarian cancer: effect on cell proliferation, invasion and clinical outcome.

PLoS ONE, 2012
Despite being an essential vitamin, folate has been implicated to enhance tumor growth, as evidenced by reports on overexpression of folate receptor alpha (FRα) in carcinomas.
Michelle K Y Siu, Daniel S H Kong, Hoi Yan Chan, Esther S Y Wong, Philip P C Ip, LiLi Jiang, Hextan Y S Ngan, Xiao-Feng Le, Annie N Y Cheung   +8 more
doaj   +1 more source

Molecular cloning and tissue distribution of reduced folate carrier and effect of dietary folate supplementation on the expression of reduced folate carrier in laying hens

Poultry Science, 2009
The reduced folate carrier (RFC) has been postulated to be a major entity for folate transport activity in humans and other mammals. However, there are limited reports of the importance of RFC in an avian system. In the current study, therefore, the molecular cloning and tissue distribution of RFC, as well as the effect of dietary folate ...
M, Jing, G B, Tactacan, J C, Rodriguez-Lecompte, A, Kroeker, J D, House   +4 more
openaire   +2 more sources

Mutations in the reduced-folate carrier affect protein localization and stability [PDF]

Biochemical Journal, 2000
The reduced-folate-carrier (rfc) gene has been shown to be functionally important for reduced-folate transport in mammalian cells. In the present paper we describe the identification of alterations in both alleles of the rfc gene in a mutant Chinese-hamster ovary cell line deficient in methotrexate transport. One allele of the rfc gene contains a point
H, Sadlish, R C, Murray, F M, Williams, W F, Flintoff   +3 more
openaire   +2 more sources

Folic Acid Absorption Characteristics and Effect on Cecal Microbiota of Laying Hens

Frontiers in Veterinary Science, 2021
This experiment was conducted to investigate the characteristics of folic acid (FA) absorption in laying hens and the effect of FA supplementation on cecal microbiota.
Yan Bai, Rui Wang, Rui Wang, Yu Yang, Ruirui Li, Xiaotian Wu   +5 more
doaj   +1 more source

Luteolin Enhances Choroid Plexus 5-MTHF Brain Transport to Promote Hippocampal Neurogenesis in LOD Rats

Frontiers in Pharmacology, 2022
Folates, provided by food, are commonly used antidepressant synergists in late-onset depression (LOD). However, increased intake of folic acid in the elderly population might lead to the accumulation of unmetabolized folic acid in the systemic ...
Hui-Zhen Li, Kai-Ge Liu, Ning-Xi Zeng, Xiao-Feng Wu, Wen-Jun Lu, Han-Fang Xu, Can Yan, Li-Li Wu   +7 more
doaj   +1 more source

Folate receptor and human reduced folate carrier expression in HepG2 cell line exposed to fumonisin B1 and folate deficiency [PDF]

Carcinogenesis, 2007
Fumonisin B1 (FB1) induces apoptosis and decreases the cellular uptake of 5-methyltetrahydrofolate. Two folate transporters (folate receptor, FR, and Reduced Folate Carrier, hRFC1) are involved in the cell uptake of folate. We aimed to study whether FB1 modifies the expression of the FR and the hRFC1 and whether its apoptotic effect is influenced by ...
Afif M, Abdel Nour, Diana, Ringot, Jean-Louis, Guéant, Abalo, Chango   +3 more
openaire   +2 more sources

Quantitative correlation between promoter methylation and messenger RNA levels of the reduced folate carrier

BMC Cancer, 2008
Background Methotrexate (MTX) uptake is mediated by the reduced folate carrier (RFC). Defective drug uptake in association with decreased RFC expression is a common mechanism of MTX resistance in many tumor types.
Kheradpour Albert, Banerjee Debabrata, Kim Hansoo, Hoang Bang H, Li Wei-Wei, Yang Rui, Healey John H, Meyers Paul A, Bertino Joseph R, Gorlick Richard   +9 more
doaj   +1 more source