Catabolic Reductive Dehalogenase Substrate Complex Structures Underpin Rational Repurposing of Substrate Scope [PDF]
Microorganisms, 2020 Reductive dehalogenases are responsible for the reductive cleavage of carbon-halogen bonds during organohalide respiration. A variety of mechanisms have been proposed for these cobalamin and [4Fe-4S] containing enzymes, including organocobalt, radical ...
Tom Halliwell +4 more
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Stoichiometry of the Gene Products From the Tetrachloroethene Reductive Dehalogenase Operon pceABCT [PDF]
Frontiers in Microbiology, 2022 Organohalide respiration (OHR) is a bacterial anaerobic process that uses halogenated compounds, e.g., tetrachloroethene (PCE), as terminal electron acceptors.
Lorenzo Cimmino +3 more
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Presence, Diversity, and Enrichment of Respiratory Reductive Dehalogenase and Non-respiratory Hydrolytic and Oxidative Dehalogenase Genes in Terrestrial Environments [PDF]
Frontiers in Microbiology, 2019 Organohalide-respiring bacteria have been linked to the cycling and possible respiration of chlorinated natural organic matter (Cl-NOM) in uncontaminated soils and sediments. The importance of non-respiratory hydrolytic/oxidative dechlorination processes
Hanna R. Temme +2 more
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Sister Dehalobacter Genomes Reveal Specialization in Organohalide Respiration and Recent Strain Differentiation Likely Driven by Chlorinated Substrates [PDF]
Frontiers in Microbiology, 2016 The genomes of two closely related Dehalobacter strains (strain CF and strain DCA) were assembled from the metagenome of an anaerobic enrichment culture that reductively dechlorinates chloroform (CF), 1,1,1-trichloroethane (1,1,1-TCA) and 1,1 ...
Shuiquan eTang +6 more
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A bacterial chloroform reductive dehalogenase: purification and biochemical characterization [PDF]
Microbial Biotechnology, 2017 Summary We report herein the purification of a chloroform (CF)‐reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23‐fold increase in relative purity compared
Bat‐Erdene Jugder +6 more
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Dechlorination of mixed chlorinated organophosphate esters (V6 and TCEP) and associated reductive dehalogenase gene expression by Dehalococcoides mccartyi [PDF]
Applied and Environmental MicrobiologyDehalococcoides mccartyi (D. mccartyi) plays a critical role in the dechlorination of halogenated organic pollutants, yet its performance on mixed chlorinated organophosphate esters (Cl-OPEs) remains poorly understood.
Sen Yang +9 more
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Detoxification of 1,1,2-trichloroethane to ethene by desulfitobacterium and identification of its functional reductase gene. [PDF]
PLoS ONE, 2015 1,1,2-trichloroethane (1,1,2-TCA) has become a common groundwater pollutant due to historically extensive utilization, improper disposal, as well as from incomplete dechlorination of 1,1,2,2-tetrachloroethane.
Siyan Zhao, Chang Ding, Jianzhong He
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Dual Element (C/Cl) Isotope Analysis Indicates Distinct Mechanisms of Reductive Dehalogenation of Chlorinated Ethenes and Dichloroethane in Dehalococcoides mccartyi Strain BTF08 With Defined Reductive Dehalogenase Inventories [PDF]
Frontiers in Microbiology, 2020 Dehalococcoides mccartyi strain BTF08 has the unique property to couple complete dechlorination of tetrachloroethene and 1,2-dichloroethane to ethene with growth by using the halogenated compounds as terminal electron acceptor. The genome of strain BTF08
Steffi Franke +4 more
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Deciphering reductive dehalogenase specificity through targeted mutagenesis of chloroalkane reductases [PDF]
Applied and Environmental MicrobiologyReductive dehalogenases (RDases) are essential in the anaerobic degradation of various organohalide contaminants. This family of enzymes has broad sequence diversity, but high structural conservation. There have been few studies assessing how RDase amino
Katherine J. Picott +2 more
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Distribution of Dehalococcoidia in the Anaerobic Deep Water of a Remote Meromictic Crater Lake and Detection of Dehalococcoidia-Derived Reductive Dehalogenase Homologous Genes. [PDF]
PLoS ONE, 2016 Here we describe the natural occurrence of bacteria of the class Dehalococcoidia (DEH) and their diversity at different depths in anoxic waters of a remote meromictic lake (Lake Pavin) using 16S rRNA gene amplicon sequencing and quantitative PCR ...
Corinne Biderre-Petit +10 more
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